Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados International Journal of Morphology (2) BABT (1) BJMBR (1) Electron. J. Biotechnol. (1) Autor Jahanshahi,M (2) Azami,N. S (1) Babakordi,F (1) Babar,E. (1) Cardillo,Alejandra B. (1) Deljo,Ali (1) Ghiasvand,Taiebeh (1) Giulietti,Ana M. (1) Habibi,Peyman (1) Marconi,Patricia L. (1) Melik,E. (1) Moghadam,Yaser Ahmadi (1) Moharreri,A (1) Nickmahzar,E (1) Nickmahzar,E. G (1) Piri,Khosro (1) Seif-hoseini,S (1) Özgünen,T. (1) Mais... Palavra-chave Scopolamine (5) Hippocampus (2) Rat (2) Acetylcholine (1) Amnesia (1) Astrocyte (1) Atropa belladonna (1) Avoidance memory (1) Bioreactor (1) H6H (1) Hairy root (1) Hairy roots (1) Hyoscyamine (1) Inhibitory avoidance (1) M1 receptor (1) Median raphe nucleus (1) Sequence analysis (1) Serotonin (1) Transformation (1) Working memory (1) Mais... Tipo do documento Journal article (3) Info:eu-repo/semantics/article (2) Ano 2015 (1) 2013 (1) 2012 (1) 2006 (1) 2002 (1) País Chile (3) Brazil (2) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Analysis and sequencing of h6hmRNA, last enzyme in the tropane alkaloids pathway from anthers and hairy root cultures of Brugmansia candida (Solanaceae) Electron. J. Biotechnol. Cardillo,Alejandra B.; Giulietti,Ana M.; Marconi,Patricia L.. Brugmansia candida (Solanaceae) is a native tree distributed across South-American and produces the pharmacologically- important group of tropane alkaloids including scopolamine. This biocompound is synthesised from hyoscyamine by action of Hyoscyamine 6-&beta; hydroxylase (H6H, EC 1.14.11.11) at the end of the tropane alkaloid pathway. Here are reported the tissue and organ-specific expression of h6hmRNA by RT-PCR analyses and the isolation, cloning and sequencing of the cDNA obtained from B. candida anthers and hairy root transformed cultures. Bioinformatic analysis of the nucleotide sequence revealed an uninterrupted ORF of 1038 bp and the predicted aminoacid sequence could be 344 aminoacid long. A database search showed that this sequence has high... Tipo: Journal article Palavras-chave: H6H; Hairy roots; Hyoscyamine; Scopolamine; Sequence analysis; Transformation. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300004 Scopolamine Reduces the Density of M1 Muscarinic Neurons in Rats' Hippocampus International Journal of Morphology Jahanshahi,M; Nickmahzar,E. G; Seif-hoseini,S; Babakordi,F; Moharreri,A. Cholinergic system in CNS is involved in learning and memory. Scopolamine as muscarinic acetylcholine receptor antagonist is used for creation of memory impairment. The purpose of this study is evaluation of scopolamine-based amnesia on memory retention and the effect of this phenomenon on the number of neurons contains M1-receptors in the male Wistar rats hippocampal regions. Thirty-five male Wistar rats (200±20 g) were distributed randomly into five groups. Control group (intact samples) and 3 experimental groups with sham group (saline) were tested by the method of passive avoidance (shuttle box) in doses of 0.2, 0.5 and 1 mg/kg (intraperitoneally) as a single dose. After one week, memory test was taken from the rats. Finally, brains dissected from... Tipo: Journal article Palavras-chave: M1 receptor; Scopolamine; Avoidance memory; Hippocampus; Rat. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400014 Effect of Scopolamine-based Amnesia on the Number of Astrocytes in the Rat's Hippocampus International Journal of Morphology Jahanshahi,M; Azami,N. S; Nickmahzar,E. As neuron­astrocyte interactions play a crucial role in the adult brain, it is thought that astrocytes support learning and memory through specific mechanisms. In this study, the effect of scopolamine based amnesia on the number of astrocytes in rats' hippocampus was studied. Adult male albino Wistar rats were bilaterally cannulated into the CA1 region and animals received saline or different doses of scopolamine (0.5, 1 and 2 mg/ rat, intra - CA1), immediately after training. Then all the rats were sacrificed and coronal sections were taken from the dorsal hippocampal formation of the right cerebral hemispheres and stained with PTAH. The area densities of the astrocytes in dentate gyrus were measured and compared in the all groups (p < 0.05). Data... Tipo: Journal article Palavras-chave: Scopolamine; Amnesia; Astrocyte; Hippocampus; Rat. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200004 Increasing Scopolamine content in Hairy Roots of Atropa belladonna using Bioreactor BABT Habibi,Peyman; Piri,Khosro; Deljo,Ali; Moghadam,Yaser Ahmadi; Ghiasvand,Taiebeh. The aim of this study was to use the, hairy root system for increasing the scopolamine content in Atropa belladonna. Agrobacterium rhizogenes ATCC15834 was utilized to produce hairy roots. The culture was carried out in a 1.5-l bioreactor using the inoculum size of 0.5 g fr. wt of 10-day-old hairy roots and various parameters, including agitation, aeration, conductivity and the consumption of sucrose were evaluated. Results revealed that the highest amount of scopolamine production (1.59 mg/g-1 dry wt) occurred in the bioreactor with aeration and agitation 1.25 vvm (volume per volume per minute) and 70 rpm, respectively. Study of conductivity and the consumption of sucrose showed that the highest amount of sucrose consumption and the highest amount of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Atropa belladonna; Hairy root; Scopolamine; Bioreactor. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200166 Excitotoxic median raphe lesions aggravate working memory storage performance deficits caused by scopolamine infusion into the dentate gyrus of the hippocampus in the inhibitory avoidance task in rats BJMBR Babar,E.; Melik,E.; Özgünen,T.. The interactions between the median raphe nucleus (MRN) serotonergic system and the septohippocampal muscarinic cholinergic system in the modulation of immediate working memory storage performance were investigated. Rats with sham or ibotenic acid lesions of the MRN were bilaterally implanted with cannulae in the dentate gyrus of the hippocampus and tested in a light/dark step-through inhibitory avoidance task in which response latency to enter the dark compartment immediately after the shock served as a measure of immediate working memory storage. MRN lesion per se did not alter response latency. Post-training intrahippocampal scopolamine infusion (2 and 4 µg/side) produced a more marked reduction in response latencies in the lesioned animals compared to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Working memory; Median raphe nucleus; Serotonin; Scopolamine; Acetylcholine; Inhibitory avoidance. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400012 Registros recuperados: 5 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco