|
|
|
Registros recuperados: 46 | |
|
|
FONSECA,María Isabel; ZAPATA,Pedro Darío; VILLALBA,Laura Lidia; FARIÑA,Julia Inés. |
This research aimed to evaluate the potential of several native white rot fungi (WRF) isolated from subtropical environments of Misiones (Argentina) to produce different ligninolytic enzymes. Coriolus versicolor f. antarcticus BAFC 266, Pycnoporus sanguineus BAFC 2126 and Phlebia brevispora BAFC 633 showed the highest phenoloxidase activity. Ganoderma applanatum strain E, P. sanguineus BAFC 2126 and P. brevispora BAFC 633 revealed marked laccase and peroxidase activity. C. versicolor f. antarcticus, G. applanatum (strain A) and Trametes villosa, gave high positive reactions with 2,6-dimethoxyphenol oxidation at the lowest tested pH. C. versicolor f. antarcticus, G. applanatum strains D and F, T. elegans BAFC 2127and T. villosa, showed the highest manganese... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oxidative enzymes; Screening; White-rot fungi. |
Ano: 2015 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000100006 |
| |
|
|
Fabre,Bibiana Raquel; Boero,Laura Estela; Argibay,Jenny; Benencia,Haydée; Del Río,Alberto. |
La prolactina (PRL), de acuerdo a su tamaño molecular, se puede encontrar en circulación como un monómero de 23 kDa (little PRL), un dímero de 45 kDa (big PRL) o como macroprolactina ( big-big PRL o MPRL). La MPRL, en la mayoría de los casos, está formada por una molécula monomérica y una inmunoglobulina G (IgG). La cromatografía por filtración en gel se considera el método patrón para determinar las diferentes formas moleculares. La precipitación con polietilenglicol (PEG) al 25% resulta un método alternativo para realizar el screening de MPRL. El objetivo de este trabajo fue establecer un valor de corte para determinar la presencia de MPRL utilizando PEG y el sistema IRMA DPC para dosar PRL. Se analizaron 172 sueros: 20 muestras con niveles de PRL... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Macroprolactina; Screening; Heterogeneidad molecular. |
Ano: 2006 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572006000100012 |
| |
|
| |
|
|
Sorgatto,Vanessa Ghiggi; Carvalho,Julio Cesar de; Sydney,Eduardo Bittencourt; Medeiros,Adriane Bianchi Pedroni; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo. |
Abstract We present an improved method of direct transesterification suitable for the quantitative analysis of multiple dry samples for its fatty acid content, using a minimal amount of biomass and reactants. The method features an acid-catalyzed direct alcoholysis of microgram samples of dry biomass; the rationale behind the solvent and reagent proportions chosen is discussed. The method was validated using seven microbial strains with diverse lipid content (Saccharomyces cerevisiae, Saccharomyces boulardii, Candida tropicalis, Haematococcus pluvialis, Chlorella vulgaris, Spirulina platensis and Schizochytrium limacinum), and compared with a macroscale direct transesterification method, and with gravimetric analysis of lipids extracted with solvents. The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbial oil; Ethanolysis; FAEE; Microscale; Screening. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100401 |
| |
|
| |
|
|
Ferreira,Michele Berger; de-Paris,Fernanda; Paiva,Rodrigo Minuto; Nunes,Luciana de Souza. |
ABSTRACT Group B Streptococcus is a causative agent of invasive neonatal infections. Maternal colonization by Streptococcus agalactiae is a necessary condition for vertical transmission, with efficient screening of pregnant women playing an essential role in the prevention of neonatal infections. In this study, we aimed to compare the performance of conventional polymerase chain reaction and real-time PCR assays as screening methods for S. agalactiae in pregnant women against the microbiological culture method considered as the gold-standard. A total of 130 samples from pregnant women were analyzed for sensitivity, specificity, positive predictive value, and negative predictive value. Statistical analysis was performed using the SPSS software, version... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus agalactiae; Pregnant women; Screening; Conventional PCR; Real-time PCR. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000600449 |
| |
|
| |
|
|
Çiftdoğan,Dilek Yılmaz; Aslan,Selda. |
Abstract Background and aim Brucellosis is an infectious, contagious and zoonotic disease that occurs worldwide. The family members of an index case of brucellosis may be especially susceptible, due to sharing the same source of infection and similar risk factors for brucellosis. In this study, we propose to screen pediatric and adult family members of brucellosis index cases for detecting additional unrecognized infected family members. Materials and methods 114 family members of 41 pediatric patients with brucellosis were evaluated. All family members completed a brief questionnaire and were tested by a standard tube agglutination test (STA). Results The majority of family members (n = 96, 84.2%) were children. Among the 114 family members, 42 (36.8%)... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucellosis; Screening; Unrecognized; Turkey. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500520 |
| |
|
|
Khawali,C.; Andriolo,A.; Ferreira,S.R.G.. |
We tested the correlation of the albumin-to-creatinine ratio (A/C) in an early-morning urine sample, measured with a commercial kit (DCA 2000®), with the conventional immunoturbidimetric determination in the laboratory and with overnight albumin excretion rate (reference method). Fifty-five type 1 diabetic adolescents had their first-morning urine collected on the 1st and 8th day of the period. Urinary albumin and creatinine were determined immediately using the DCA 2000® kit. Samples were also stored for laboratory analysis. To evaluate the correlation between early-morning urinary A/C ratio and overnight albumin excretion rate, 16 subjects had a timed overnight urine collection. A/C ratios determined with the DCA 2000® kit and by the laboratory method... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diabetic nephropathy; Microalbuminuria; Screening. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300008 |
| |
|
|
Suffredini,I.B.; Sader,H.S.; Gonçalves,A.G.; Reis,A.O.; Gales,A.C.; Varella,A.D.; Younes,R.N.. |
More than 20% of the world's biodiversity is located in Brazilian forests and only a few plant extracts have been evaluated for potential antibacterial activity. In the present study, 705 organic and aqueous extracts of plants obtained from different Amazon Rain Forest and Atlantic Forest plants were screened for antibacterial activity at 100 µg/ml, using a microdilution broth assay against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli. One extract, VO581, was active against S. aureus (minimum inhibitory concentration (MIC) = 140 µg/ml and minimal bactericidal concentration (MBC) = 160 µg/ml, organic extract obtained from stems) and two extracts were active against E. faecalis, SM053 (MIC = 80 µg/ml and MBC = 90... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant extract; Antibacterial activity; Screening; Amazon Rain Forest; Atlantic Forest. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300015 |
| |
|
|
Chuang,K.A.; Lieu,C.H.; Tsai,W.J.; Wu,M.H.; Chen,Y.C.; Liao,J.F.; Wang,C.C.; Kuo,Y.C.. |
Refractory and relapsed leukemia is a major problem during cancer therapy, which is due to the aberrant activation of Wnt/β-catenin signaling pathway. Activation of this pathway is promoted by wingless (Wnt) proteins and induces co-activator β-catenin binding to lymphoid enhancer factor (LEF)/T-cell factor protein (TCF). To provide a convenient system for the screening of anti-Wnt/β-catenin agents, we designed a bi-functional pGL4-TOP reporter plasmid that contained 3X β-catenin/LEF/TCF binding sites and a selectable marker. After transfection and hygromycin B selection, HEK 293-TOP and Jurkat-TOP stable clones were established. The luciferase activity in the stable clone was enhanced by the recombinant Wnt-3A (rWnt-3A; 100-400 ng/mL) and GSK3β inhibitor... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cancer; Plasmids; Reporter assay; Screening; Wnt; Β-catenin. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001000003 |
| |
|
|
Li,Min; Kong,Yan; Chen,Hongcun; Chu,Aiqin; Song,Guiqi; Cui,Yan. |
Sarcopenia remains poorly managed in clinical practice due to the lack of simple and accurate screening tools. This study aimed to identify the cutoff values of the SARC-F questionnaire and Ishii's score using the variables age, grip strength, and calf circumference in older inpatients in China to compare the accuracy of the two methods and to explore their predictive ability for adverse outcomes (rehospitalization, falls, fracture, and death). Hospitalized patients (n=138) aged ≥60 years were included. The accuracy of the two tools was evaluated using the reference diagnosis recommended by the Asian Working Group on Sarcopenia (assessing patients with measurements of muscle mass, handgrip strength, and usual gait speed). Follow-up data were obtained by... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sarcopenia; Muscle mass; Geriatric inpatients; Screening; Score. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900606 |
| |
|
|
Mazza Nascimento,M.; Riella,M.C.. |
The incidence and prevalence of chronic kidney disease have been increasing in recent years in developing countries. The aim of this study was to report the results of a general chronic kidney disease awareness program applied to an urban population in a large Brazilian city. From January 2002 to January 2005 a total of 8883 individuals in the city of Curitiba (PR, Brazil) were screened for hypertension, body mass index, hematuria, and proteinuria. A family history and previous medical diagnosis of hypertension and diabetes mellitus (DM) were also recorded. Of the 8883 individuals assessed, 56% were women, subject median age was 47 years (range: 17-93 years) and more than 90% were Caucasian. Thirty percent had signs of hematuria, 6% had proteinuria, and 3%... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hematuria; Proteinuria; Prevention; Chronic kidney disease; Screening. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000800010 |
| |
|
|
Golfetto,L.; Alves,E.V.; Martins,T.R.; Sincero,T.C.M.; Castro,J.B.S.; Dannebrock,C.; Oliveira,J.G.; Levi,J.E.; Onofre,A.S.C.; Bazzo,M.L.. |
Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women were analyzed. One sample was used for conventional cytology and another sample was collected using BD SurePath™ kit for HPV tests. Eighty samples (24.6%) were positive for HPV gene by PCR-Multiplex and were then submitted to PCR-RFLP and PapilloCheck® microarray. There was a genotyping agreement in 71.25% (57/80) on at least one HPV type between PCR-RFLP and PapilloCheck® microarray. In 22 samples... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cervical cancer; HPV; Screening; PCR-RFLP; PapilloCheck® microarray. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500619 |
| |
|
|
Lourenço da Silva,A.; Elisabetsky,E.. |
Experimental drugs and/or plant extracts are often dissolved in solvents, including propylene glycol. Nevertheless, there is evidence for psychoactive properties of this alcohol. In this study we found that in the hole-board test 10% propylene glycol did not modify the head-dipping behavior. However, 30% propylene glycol induced an increase in the number of head-dips (46.92 ± 2.37 compared to 33.83 ± 4.39, P<0.05, ANOVA/Student-Newman-Keuls), an effect comparable to that obtained with 0.5 mg/kg diazepam (from 33.83 ± 4.39 to 54 ± 3.8, P<0.01, ANOVA/Student-Newman-Keuls). These results demonstrate that 30% propylene glycol has significant anxiolytic effects in this model and therefore cannot be used as an innocuous solvent. |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Screening; Anxiolytic drugs; Propylene glycol; Diazepam; Solvents; Hole-board. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400016 |
| |
|
| |
|
|
Zhang,J.; Ma,M.; Nan,X.; Sheng,B.. |
Serum prostate-specific antigen (PSA) is a diagnostic biomarker of prostate cancer and is possibly associated with obesity. This study aimed to explore the relationships between obesity indicators [body mass index (BMI) and waist circumference (WC)] with PSA in Chinese men. A cross-sectional study of men aged 30-85 years undergoing prostate cancer screening was conducted from August 2008 to July 2013 in Xi'an, China. Data were obtained from clinical reports, condition was recorded based on self-report including demographics, weight, height, and WC (>90 cm=obese). Fasting blood glucose (FBG) and prostate volume (PV) were assessed clinically. Patients were grouped by BMI (normal=22.9, overweight=23-27.4, obese≥27.5 kg/m2). PSA parameters of density... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prostate-specific antigen; Body mass index; Obesity; Prostate cancer; Screening. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800704 |
| |
|
|
Li,Shi Lin; Lin,Qiang; Li,Xin Ran; Xu,Hui; Yang,Yun Xi; Qiao,Dai Rong; Cao,Yi. |
Microbial lipids, which are also known as single cell oils (SCO), are produced by oleaginous microorganisms including oleaginous bacteria, yeast, fungus and algae through converting carbohydrates into lipids under certain conditions. Due to its unique environment having extremely low temperature and anoxia, the Tibetan Plateau is amongst the regions with numerous rare ecotypes such as arid desert, salt marsh, alpine permafrost, hot spring, and lawn. By using a rapid, convenient screening method, we identified 31 strains of oleaginous microorganisms from different habitats in the Tibetan Plateau, which include wetlands, lawn, hot spring, alpine permafrost, and saline-alkali soil. Molecular identity analysis showed that they belong to 15 different species, 7... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oleaginous microorganisms; Screening; The Tibetan Plateau; Biodiversity. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200026 |
| |
|
|
Koc,Melih; Cokmus,Cumhur; Cihan,Arzu Coleri. |
Abstract Thermophilic 32 isolates and 20 reference bacilli were subjected to Rep-PCR and ITS-PCR fingerprinting for determination of their genotypic diversity, before screening lipase activities. By these methods, all the isolates and references could easily be differentiated up to subspecies level from each other. In screening assay, 11 isolates and 7 references were found to be lipase producing. Their extracellular lipase activities were measured quantitatively by incubating in both tributyrin and olive oil broths at 60 °C and pH 7.0. During the 24, 48 and 72-h period of incubation, the changes in the lipase activities, culture absorbance, wet weight of biomass and pH were all measured. The activity was determined by using pNPB in 50 mM phosphate buffer... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endospore-forming bacilli; Screening; Thermostable lipase; ITS-PCR; Rep-PCR. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401065 |
| |
|
|
Benassi,Vivian Machado; Lucas,Rosymar Coutinho de; Jorge,João Atílio; Polizeli,Maria de Lourdes Teixeira de Moraes. |
Plant cell wall is mainly composed by cellulose, hemicellulose and lignin. The heterogeneous structure and composition of the hemicellulose are key impediments to its depolymerization and subsequent use in fermentation processes. Thus, this study aimed to perform a screening of thermophilic and thermotolerant filamentous fungi collected from different regions of the São Paulo state, and analyze the production of β-xylosidase and arabinanase at different temperatures. These enzymes are important to cell wall degradation and synthesis of end products as xylose and arabinose, respectively, which are significant sugars to fermentation and ethanol production. A total of 12 fungal species were analyzed and 9 of them grew at 45 ºC, suggesting a thermophilic or... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Screening; Β-D-xylosidase; Arabinanase; Thermotolerant; Thermophilic. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400042 |
| |
Registros recuperados: 46 | |
|
|
|