Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Genet. Mol. Biol. (2) Electron. J. Biotechnol. (1) Autor Huang,Huiyang (2) Yang,Ya'nan (2) Ye,Haihui (2) Gong,Jie (1) Jiang,Keji (1) Ma,Lingbo (1) Pi,Yan (1) Wang,Guizhong (1) Xie,Yinjie (1) Yu,Kun (1) Zeng,Xianglan (1) Zhang,Dan (1) Zhang,Fengying (1) Zhu,Houze (1) Mais... Palavra-chave Scylla paramamosain (3) Chymotrypsin (1) Fatty acid-binding protein (1) Immune response (1) Immunity (1) Immunocytochemistry (1) In situ hybridization (1) Optic stalk (1) Ovary development (1) Potassium acetate (1) RNA isolation (1) Real-time quantitative PCR (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Journal article (1) Ano 2014 (1) 2013 (1) 2012 (1) País Brazil (2) Chile (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Improved isolation of good-quality total RNA from the optic stalk of Mud crab, Scylla paramamosain Electron. J. Biotechnol. Zhu,Houze; Jiang,Keji; Zhang,Fengying; Zhang,Dan; Pi,Yan; Ma,Lingbo. An improved and efficient protocol was developed based on the TaKaRa RNAiso Plus Kit (Code: D9108A) for isolating good-quality total RNA from the optic stalk of mud crab, Scylla paramamosain. The protocol was based on the Trizol method with modifications. The carapace overlapping the optic stalk was retained with RNA in regular protocol. In order to remove the abundant deposition correlative with the carapace which makes the isolation of RNA particularly difficult, 5M potassium acetate solution (pH = 6.0) was added before the precipitation of RNA, and the temperature of RNA deposition was also decreased to -70ºC to ensure the stabilization of RNA. Good-quality total RNA from the optic stalk of S. paramamosain could be easily isolated with this modified... Tipo: Journal article Palavras-chave: Optic stalk; Potassium acetate; RNA isolation; Scylla paramamosain. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200005 Molecular cloning and functional analysis of the fatty acid-binding protein (Sp-FABP) gene in the mud crab (Scylla paramamosain) Genet. Mol. Biol. Zeng,Xianglan; Ye,Haihui; Yang,Ya'nan; Wang,Guizhong; Huang,Huiyang. Intracellular fatty acid-binding proteins (FABPs) are multifunctional cytosolic lipid-binding proteins found in vertebrates and invertebrates. In this work, we used RACE to obtain a full-length cDNA of Sp-FABP from the mud crab Scylla paramamosain. The open reading frame of the full length cDNA (886 bp) encoded a 136 amino acid polypeptide that showed high homology with related genes from other species. Real-time quantitative PCR identified variable levels of Sp-FABP transcripts in epidermis, eyestalk, gill, heart, hemocytes, hepatopancreas, muscle, ovary, stomach and thoracic ganglia. In ovaries, Sp-FABP expression increased gradually from stage I to stage IV of development and decreased in stage V. Sp-FABP transcripts in the hepatopancreas and hemocytes... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fatty acid-binding protein; Immunity; Ovary development; Real-time quantitative PCR; Scylla paramamosain. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100021 Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain Genet. Mol. Biol. Gong,Jie; Xie,Yinjie; Yu,Kun; Yang,Ya'nan; Huang,Huiyang; Ye,Haihui. Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequence and encoded a peptide of 270 amino acids with a signal peptide of 17 amino acids. The SpCHY gene contains seven exons, six introns, a TATA box and several transcription factor binding sites that were found in 5'-promoter region which is 1221 bp in length-time quantitative PCR analysis indicated that the expression level of SpCHY mRNA in hepatopancreas was significantly higher than that in other tissues. Immunocytochemistry and in situ hybridization... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chymotrypsin; Scylla paramamosain; Immune response; Immunocytochemistry; In situ hybridization. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300010 Registros recuperados: 3 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco