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	Provedor de dados BJMBR (1) J. Venom. Anim. Toxins incl. Trop. Dis. (1) Autor Bernáldez-Sarabia,Johanna (1) Cerveñansky,C. (1) Duran,R. (1) Freitas,J.C. (1) Lagos,P. (1) Licea-Navarro,Alexei (1) Pedraza-Alva,Gustavo (1) Pérez-García,Erick I. (1) Pérez-Martínez,Leonor (1) Ramírez-Carreto,Santos (1) Rodríguez-Almazán,Claudia (1) Rudiño-Piñera,Enrique (1) Salazar-García,Sandra I. (1) Silveira,R. (1) Mais... Palavra-chave Sea anemone (2) Anthopleura (1) Bunodosoma cangicum (1) Crab nerve (1) Hemolytic activity (1) Lung carcinoma (1) Neurotoxins (1) Pore-forming protein (1) Venom (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2019 (1) 2001 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Identification of a pore-forming protein from sea anemone Anthopleura dowii Verrill (1869) venom by mass spectrometry J. Venom. Anim. Toxins incl. Trop. Dis. Ramírez-Carreto,Santos; Pérez-García,Erick I.; Salazar-García,Sandra I.; Bernáldez-Sarabia,Johanna; Licea-Navarro,Alexei; Rudiño-Piñera,Enrique; Pérez-Martínez,Leonor; Pedraza-Alva,Gustavo; Rodríguez-Almazán,Claudia. Abstract Background: Pore-forming proteins (PFP) are a class of toxins abundant in the venom of sea anemones. Owing to their ability to recognize and permeabilize cell membranes, pore-forming proteins have medical potential in cancer therapy or as biosensors. In the present study, we showed the partial purification and sequencing of a pore-forming protein from Anthopleura dowii Verrill (1869). 17. Methods: Cytolytic activity of A. dowii Verrill (1869) venom was determined via hemolysis assay in the erythrocytes of four mammals (sheep, goat, human and rabbit). The cytotoxic activity was analyzed in the human adherent lung carcinoma epithelial cells (A549) by the cytosolic lactate dehydrogenase (LDH) assay, and trypan blue staining. The venom was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthopleura; Pore-forming protein; Venom; Lung carcinoma; Sea anemone. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100301 Identification of hemolytic and neuroactive fractions in the venom of the sea anemone Bunodosoma cangicum BJMBR Lagos,P.; Duran,R.; Cerveñansky,C.; Freitas,J.C.; Silveira,R.. Sea anemones are a rich source of biologically active substances. In crayfish muscle fibers, Bunodosoma cangicum whole venom selectively blocks the I K(Ca) currents. In the present study, we report for the first time powerful hemolytic and neuroactive effects present in two different fractions obtained by gel-filtration chromatography from whole venom of B. cangicum. A cytolytic fraction (Bcg-2) with components of molecular mass ranging from 8 to 18 kDa elicited hemolysis of mouse erythrocytes with an EC50 = 14 µg/ml and a maximum dose of 22 µg/ml. The effects of the neuroactive fraction, Bcg-3 (2 to 5 kDa), were studied on isolated crab nerves. This fraction prolonged the compound action potentials by increasing their duration and rise time in a... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bunodosoma cangicum; Sea anemone; Hemolytic activity; Neurotoxins; Crab nerve. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700009 Registros recuperados: 2 Primeira ... 1 ... Última

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