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	Provedor de dados ArchiMer (2) Braz. J. Plant Physiol. (1) Electron. J. Biotechnol. (1) Genet. Mol. Biol. (1) RChHN (1) Autor Beltramini,Leila Maria (1) Brumano,Maria Helena Nasser (1) Fan, Tung-yung (1) Fonseca,Miguel M. (1) Fortes, Miguel (1) GONZÁLE,BERNARDO (1) GÓMEZ,PATRICIA (1) Harii, Saki (1) Harris,D. James (1) LAVIN,PARIS (1) Mallu,Maheswara Reddy (1) Nadaoka, Kazuo (1) Nagai, Satoshi (1) Nakaema,Marcelo Kiyoshi Kian (1) Oliveira,Maria Goreti de Almeida (1) Oliveira,Marli Lourdes de (1) Pires,Christiano Vieira (1) Ronda,Srinivasa Reddy (1) Silva-Lucca,Rosemeire Aparecida (1) Simone,Salvatore Giovanni de (1) Mais... Palavra-chave Secondary structure (6) Amphibians (1) Biological activity (1) Cell lysis (1) Circular dichroism (1) Concerted evolution (1) Cross flow filtration (1) Cyanobacteria (1) DGGE (1) DNA sequence data (1) Data sets (1) Evolutionary trees (1) Fed-batch fermentation (1) Gene rearrangements (1) ITS (1) ITS2 (1) Inferring phylogenies (1) Leguminosae (1) Lenght difference test (1) Maximum likelihood estimation (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Journal article (2) Text (2) Ano 2016 (1) 2015 (1) 2010 (1) 2008 (2) 2003 (1) País Brazil (2) Chile (2) France (2) Idioma Inglês (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Production, purification and characterization of recombinant human antithrombin III by Saccharomyces cerevisiae Electron. J. Biotechnol. Mallu,Maheswara Reddy; Vemula,Sandeep; Ronda,Srinivasa Reddy. Background: Antithrombin III (ATIII) is a protein that inhibits abnormal blood clots (or coagulation) by breaking down thrombin and factor Xa. ATIII helps to keep a healthy balance between hemorrhage and coagulation. The present work demonstrated the production, purification and characterization of recombinant human antithrombin (rhAT) from yeast Saccharomyces cerevisiae BY4741 was demonstrated. After expression of rhAT by S. cerevisiae, the biomass and rhAT concentration were analyzed through fed-batch fermentation process. Results: In fed-batch fermentation, the biomass (maximum cell dry weight of 11.2 g/L) and rhAT concentration (312 mg/L) of the expressed rhAT were achieved at 84 h of cultivation time. The maximum cell lysis efficiency (99.89%) was... Tipo: Journal article Palavras-chave: Biological activity; Cell lysis; Cross flow filtration; Fed-batch fermentation; Purification; Secondary structure. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400012 Relationship between mitochondrial gene rearrangements and stability of the origin of light strand replication Genet. Mol. Biol. Fonseca,Miguel M.; Harris,D. James. Mitochondrial gene rearrangements are much more frequent in vertebrates than initially thought. It has been suggested that the origin of light strand replication could have an important role in the process of gene rearrangements, but this hypothesis has never been tested before. We used amphibians to test the correlation between light-strand replication origin thermodynamic stability and the occurrence of gene rearrangements. The two variables were correlated in a non-phylogenetic approach, but when tested in a phylogenetically based comparative method the correlation was not significant, although species with unstable light-strand replication origins were much more likely to have undergone gene rearrangements. This indicates that within amphibians there... Tipo: Info:eu-repo/semantics/article Palavras-chave: MtDNA replication; Gene rearrangements; Origin of replication; Secondary structure; Amphibians. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300027 Genetic diversity, paraphyly and incomplete lineage sorting of mtDNA, ITS2 and microsatellite flanking region in closely related Heliopora species (Octocorallia) ArchiMer Yasuda, Nina; Taquet, Coralie; Nagai, Satoshi; Fortes, Miguel; Fan, Tung-yung; Harii, Saki; Yoshida, Terutoyo; Sito, Yuta; Nadaoka, Kazuo. Examining genetic diversity and lineage sorting of different genes in closely related species provide useful information for phylogenetic analyses and ultimately for understanding the origins of biodiversity. In this study, we examined inter- and intraspecific genetic variation in internal transcribed spacer 2 (ITS2), partial mitochondrial gene (mtMutS), and nuclear microsatellite flanking region in two closely related octocoral species (Heliopora coerulea, HC-A and HC-B). These species were recently identified in a population genetic study using microsatellite markers. The two species have different reproductive timing, which ecologically promotes lineage sorting. In this study, we examined whether species boundaries could be detected by the commonly used... Tipo: Text Palavras-chave: ITS2; Concerted evolution; MtMutS; Secondary structure; Species identification; Speciation. Ano: 2015 URL: https://archimer.ifremer.fr/doc/00667/77932/80144.pdf Molecular phylogeny of pearl oysters and their relatives (Mollusca, Bivalvia, Pterioidea) ArchiMer Temkin, Ilya. Background: The superfamily Pterioidea is a morphologically and ecologically diverse lineage of epifaunal marine bivalves distributed throughout the tropical and subtropical continental shelf regions. This group includes commercially important pearl culture species and model organisms used for medical studies of biomineralization. Recent morphological treatment of selected pterioideans and molecular phylogenetic analyses of higher-level relationships in Bivalvia have challenged the traditional view that pterioidean families are monophyletic. This issue is examined here in light of molecular data sets composed of DNA sequences for nuclear and mitochondrial loci, and a published character data set of anatomical and shell morphological characters. Results:... Tipo: Text Palavras-chave: DNA sequence data; Maximum likelihood estimation; Lenght difference test; Subunit ribosomal RNA; Evolutionary trees; Data sets; Inferring phylogenies; Secondary structure; Substitution rates; Parsimony nalysis. Ano: 2010 URL: https://archimer.ifremer.fr/doc/00206/31686/30106.pdf Diversity of the marine picocyanobacteria Prochlorococcus and Synechococcus assessed by terminal restriction fragment length polymorphisms of 16S-23S rRNA internal transcribed spacer sequences RChHN LAVIN,PARIS; GÓMEZ,PATRICIA; GONZÁLE,BERNARDO; ULLOA,OSVALDO. In order to assess the appropriateness of the use of internal transcribed spacer (ITS) sequences for the study of population genetics of marine cyanobacteria, we amplified and cloned the 16S rRNA gene plus the 16S-23S ITS regions of six strains of Prochlorococcus and Synechococcus. We analyzed them by denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphisms (T-RFLP). When using the standard application of these techniques, we obtained more than one band or terminal restriction fragment (T-RF) per strain or cloned sequence. Reports in literature have suggested that these anomalies can result from the formation of secondary structures. Secondary structures of the ITS sequences of Prochlorococcus and Synechococcus... Tipo: Journal article Palavras-chave: Cyanobacteria; DGGE; T-RFLP; ITS; Secondary structure. Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2008000400006 Purification and partial characterization of a lectin from Caesalpinia tinctoria Domb, ex Dc fruits Braz. J. Plant Physiol. Oliveira,Marli Lourdes de; Beltramini,Leila Maria; Simone,Salvatore Giovanni de; Brumano,Maria Helena Nasser; Silva-Lucca,Rosemeire Aparecida; Nakaema,Marcelo Kiyoshi Kian; Pires,Christiano Vieira; Oliveira,Maria Goreti de Almeida. A lectin was isolated from the pod saline extract of Caesalpinia tinctoria by dialoconcentration on Centripep-10 and affinity chromatography on chitin column. The purified lectin was partially characterized with respect to its biochemical and structural properties. It contains 8.3 % of carbohydrate and exhibited an agglutinating activity against human erythrocytes (ABO groups). Its amino acid composition was characterized by a great number of acidic and hydrophobic residues and the estimated molecular mass was 12.5 kDa. The presence of only one N-terminal amino acid sequence (D¹-V-P-A-Y-V-Y-V-H-F10-G-F-G-E-E-H-R -D-V-F20-D), showed the homogeneity of the purified lectin. The far-ultraviolet circular dichroism (CD) spectrum of lectin indicated that it... Tipo: Info:eu-repo/semantics/article Palavras-chave: Circular dichroism; Leguminosae; Plant defense; Pod; Secondary structure. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000200008 Registros recuperados: 6 Primeira ... 1 ... Última

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