|
|
|
| Registros recuperados: 65 | |
|
|
| Pereira, Marcelo Maia; Universidade Federal de Viçosa; Ribeiro Filho, Oswaldo Pinto; Universidade Federal de Viçosa; Zanuncio, Jose Cola; Universidade Federal de Viçosa; Troni, Allan Reis; Universidade Federal de Viçosa; Navarro, Rodrigo Diana; Universidade de Brasília; Takamura, Angela Emi; Universidade Federal de Viçosa. |
| By understanding the hormonal action behind the induction of spermiation in Leptodactylus ocellatus can help in the collection of semen for artificial insemination and cryopreservation. The experiments were conducted at the Experimental Frog Farm of the Federal University of Viçosa. Seven L. ocellatus males with the following secondary sexual characteristics were selected: black thorns, developed forelimbs, and amplexus reflex. Spermiation was induced in these animals by applying daily 0.4 μg buserelin acetate hormone (GnRHa) for 7 days, being the semen collected 90 min after the application. The volume, color, vigor, motility, sperm concentration, and spermatozoa morphology were subsequently evaluated. Three animals responded to GnRHa induction, enabling... |
| Tipo: Estudos morfológicos |
Palavras-chave: 2.04.06.00-2; 5.05.04.00-2; 5.06.03.00-0 creole frog; GnRHa; Semen; Spermatozoon; Sperm motility Aquicultura. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777 |
| |
|
|
| Pereira, Marcelo Maia; Universidade Federal de Viçosa; Ribeiro Filho, Oswaldo Pinto; Universidade Federal de Viçosa.; Zanuncio, Jose Cola; Universidade Federal de Viçosa; Navarro, Rodrigo Diana; Universidade de Brasília; Seixas Filho, Jose Teixeira; Fundação Instituto de Pesca do Rio de Janeiro; Ribeiro, Christiano Demétrio de Lima; Universidade Federal de Viçosa. |
| Evaluation of semen characteristics after hormonal induction of the bullfrog could provide valuable information on the gametes of this species, which may be useful for projects related to artificial fertilization, animal improvement, and cryopreservation. Bullfrog males were induced to spermiate with buserelin acetate (GnRHa), and their semen was subsequently analyzed. GnRHa (0.4 μg) was administered to the bullfrog males with secondary sexual characteristics such as weight > 200 g, yellow chin, nuptial callus, and amplexus reflex, being the semen collected after 60 min. The semen volume was 5.76 mL, light-colored. The other characteristics of the semen were: vigor of 4.80, motility of 93%, concentration of 14.24 × 106 mL-1, and content of normal... |
| Tipo: Reprodução |
Palavras-chave: 5.06.03.00-0; 5.05.04.00-2; 2.04.06.00-2 reproduction; Semen; Spermatozoid; Sperm motility Aquicultura. |
| Ano: 2013 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14780 |
| |
|
|
| Carrillo-Nieto,Oscar; Domínguez-Vara,Ignacio A.; Huerta-Bravo,Maximino; Jaramillo-Escutia,Gerardo; Díaz-Zarco,Soledad; Vázquez-Armijo,José Fernando; Pescador-Salas,Nazario; Revilla-Vázquez,Alma. |
| Resumen En México se han diagnosticado deficiencias de minerales traza en ovinos en pastoreo, pero hay pocas estrategias para corregirlas y evaluar su respuesta. El objetivo de este estudio fue evaluar, en sementales ovinos (10 Hampshire con PV 80.6±7.6 kg y 8 Suffolk con PV 86.3±9.3 kg), el efecto de un suplemento de selenito de sodio en un bolo intraruminal de liberación prolongada (500 mg de Se), sobre el PV, condición corporal, circunferencia escrotal, variables hemáticas, actividad de glutatión peroxidasa, concentración de selenio en suero sanguíneo y características del eyaculado. El diseño experimental fue completamente al azar con arreglo factorial 2x2x3 sin y con Se (bolo intraruminal), razas (Hampshire y Suffolk) y tres meses en la época... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carneros; Selenito de sodio; Bolo intraruminal; Semen; Glutatión peroxidasa. |
| Ano: 2018 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952018000600827 |
| |
|
| |
|
| |
|
|
| Sousa,P.C.; Amorim,R.N.L.; Lima,G.L.; Paiva,A.L.C.; Paula,V.V.; Freitas,C.I.A.; Silva,A.R.. |
| ABSTRACT The aim was to verify the effects of different anesthetic protocols used during electroejaculation (EEJ) in six-banded armadillos (Euphractus sexcinctus). Four sexually matured animals were physically restrained and subjected to semen collection by the EEJ following three treatments: The control group consisted of no use of anesthesia; in the others, the anesthetic combinations xylazine/ketamine/propofol or butorphanol/ ketamine/propofol were administered. For each group, twelve procedures were conducted for EEJ. Semen was evaluated for volume, color, aspect, motility, sperm concentration, morphology, viability, and functional membrane integrity. The highest efficiency (100% ejaculates) was achieved when the control group was used; the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Semen; Edentate; Xenarthra; Electroejaculation. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601595 |
| |
|
|
| Rabassa,V.R.; Feijó,J.O.; Perazzoli,D.; Pereira,C.M.; Schild,A.L.P.; Lucia Júnior,T.; Corcini,C.D.; Schmitt,E.; Schneider,A.; Pino,F.A.B.D.; Bianchi,I.; Corrêa,M.N.. |
| ABSTRACT The aim of this study was to evaluate the effect of pST injections on metabolism, testicular size, and sperm characteristics in young boars. Sixty 22-day old piglets were divided into two groups: pST (n=30) and Control (n=30). The pST group was submitted to pST injections (90µg/kg body weight) every three days up to 330 days of age. Blood collections were performed weekly. Testicular weight was measures at 22, 82, 142, 202 and 365 days of age. Libido and fresh semen characteristics were evaluated between 150 and 210 days of age. Semen characteristics were also evaluated during a 72h storage period (15ºC). Testosterone, albumin, and phosphorus blood concentrations were higher in the pST group (P<0.05). The pST group had a higher IGF-I... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Swine; Growth hormone; PST; Testicle; Semen. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100073 |
| |
|
| |
|
|
| Costa,L.F.; Nozaki,C.N.; Lira,N.S.C.; Antunes,J.M.A.P.; Xavier,M.N.; Costa,E.A.; Paixão,T.A.; Santos,R.L.; Megid,J.. |
| The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams. The performance of the species-specific nested PCR was compared with the results of a genus-specific PCR. Fourteen rams were experimentally infected with the Brucella ovis REO 198 strain and samples of semen and urine were collected every week up to 180 days post infection. Out of 83 semen samples collected, 42 (50.6%) were positive for the species-specific nested PCR, and 23 (27.7%) were positive for the genus-specific PCR. Out of 75 urine samples, 49 (65.3%) were positive for the species-specific nested PCR, whereas 11 (14.6%) were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucella ovis; Species-specific; Nested PCR; Semen; Urine. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100009 |
| |
|
|
| Silva,L.C.; Assis,V.P.; Ribeiro,V.M.; Tafuri,W.L.; Toledo Júnior,J.C.; Silva,S.O.; Melo,M.N.; Rachid,M.A.; Valle,G.R.. |
| Considering the venereal transmission of visceral leishmaniasis from dogs to bitches, the aim of this study was to verify if the penile surface and smegma from infected dogs can be the source of parasites in bitches. Twelve Leishmania infantum infected dogs had semen and smegma samples collected for submission to PCR identification of the DNA of the parasite. Semen (41.7%) and smegma (50.0%) have similar positive incidence (P>0.05; Fisher's exact test), with 58.3% of the dogs positive for semen and/or smegma samples. The proportion of positivity for both semen and smegma was 33.3%, but 8.3% was positive only for semen, and 16.7% only for smegma, revealing a moderate agreement between tests (K=0.5; Kappa index). It was concluded that Leishmania infantum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania infantum; Dog; Prepuce; Smegma; Semen. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300731 |
| |
|
| |
|
|
| Gheller,S.M.M.; Corcini,C.D.; Santos,F.C.C.; Tavares,G.C.; Costa,V.G.G.; Curcio,B.R.; Nogueira,C.E.W.; Varela Junior,A.S.. |
| ABSTRACT This study was designed to evaluate the possible benefits of adding xanthan gum to a standard extender for equine through in vitro analyzes of sperm quality. Semen was collected four times from five different stallions (n= 20 samples) and subjected to cooled storage under different conditions: control (only standard extender) and three different concentrations of xanthan gum (0.01%, 0.12%, and 0.25%) supplemented to the extenders. Sperm parameters, such as motility, mitochondrial functionality, and membrane, acrosome, and DNA integrity were measured after 0h, 24h, 48h, and 72h of sperm storage at 5ºC. Our observations indicated that sperm motility declined with longer cooling period with the 0.25% xanthan gum supplementation group compared with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cooled; Stallion; Semen; Spermatozoa; Storage. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100028 |
| |
|
|
| Aguiar,G.V.; van Tilburg,M.F.; Catunda,A.G.V.; Celes,C.K.S.; Lima,I.C.S.; Campos,A.C.N.; Moura,A.A.A.; Araújo,A.A.. |
| The present study aimed to verify the caprine semen characteristics during dry and rainy seasons in the Brazilian Northeast, and the influence of these seasons on cooled semen. Seminal volume, concentration, percentage of motile cells, vigor and spermatic morphology, as well as biochemical profile (fructose, citric acid, P, Ca2+, Mg, total proteins and phospholipase A2 activity) were analyzed. It was observed a reduction (P<0.05) in normal sperm morphology, fructose, citric acid, P, Mg and total protein concentration during the dry season, which did not affect the motility, vigor, volume and sperm concentration. Phospholipase A2 activity was increased during the dry season (P<0.05). The analysis of the semen cooled at 4ºC during 48 hours showed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Semen; Spermatozoa; Season; Cooling; Caprine. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100002 |
| |
|
|
| Oliveira,E.C.S.; Juliani,G.C.; Marques Jr.,A.P.; Henry,M.. |
| The efficacy of three extenders, tris-egg yolk-5% ethylene glycol (T1), lactose-egg yolk-5% ethylene glycol (T2) and lactose-egg yolk-5% dimethyl formamide (T3) on preserving the viability of post-thawing canine spermatozoa was evaluated. Three ejaculates per dog were obtained of five animals. The semen was packaged in 0.5ml straws and cooled to 4°C for 120min. The straws were frozen 4cm above the nitrogen level for 15min and thawed in water-bath at 37°C for 60sec and at 75°C for 7sec. Progressive motility and vigour were evaluated immediately after thawing (time 0) and at 30, 60, 90 and 120min. Structural and functional integrity of plasma membrane of the spermatozoa were evaluated, respectively, by fluorescent staining probes and hypoosmotic swelling... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Semen; Cryopreservation; Ethylene glycol; Dimethyl formamide. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600021 |
| |
|
| |
|
|
| Celeghini,E.C.C.; Nascimento,J.; Raphael,C.F.; Andrade,A.F.C.; Arruda,R.P.. |
| In this experiment, it was defined a protocol of fluorescent probes combination: propidium iodide (PI), fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA), and JC-1. For this purpose, four ejaculates from three different rams (n=12), all showing motility >80% and abnormal morphology <10%, were diluted in TALP medium and split into two aliquots. One of the aliquots was flash frozen and thawed in three continuous cycles, to induce damage in cellular membranes and to disturb mitochondrial function. Three treatments were prepared with the following fixed ratios of fresh semen:flash frozen semen: 0:100 (T0), 50:50 (T50), and 100:0 (T100). Samples were stained in the proposal protocol and evaluated by epifluorescence microscopy.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovine; Semen; Epifluorescence microscopy; Sperm membranes. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000300006 |
| |
|
|
| Santos,E.A.A.; Sousa,P.C.; Peixoto,G.C.X.; Simão,B.R.; Oliveira,M.F.; Silva,A.R.. |
| Soluções hiposmóticas com diferentes concentrações (0, 50, 100, 150, 200mOsm/L) foram testadas para a avaliação funcional da membrana espermática de catetos (n=13). Foi verificado que o número de espermatozoides reagidos diminuía (P<0,05) de acordo com o aumento da osmolaridade do meio. A maior porcentagem (71,8%) de espermatozoides reagidos, bem como a menor variação nas respostas osmóticas, foi detectada com o uso de água destilada (0mOsm/L) (P<0,05), a qual também apresentou a menor variação nos resultados, de acordo com os erros padrão verificados. Em conclusão, a água destilada aparenta ser uma solução adequada para o uso no teste hiposmótico para sêmen de catetos. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Collared; Peccary; Semen; Andrology; Wild animal. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400044 |
| |
|
|
| Henzel,A.; Salla,P.F.; Mascitti,A.K.; Demoliner,M.; Solyman,M.C.; Lunge,V.R.; Spilki,F.R.. |
| ABSTRACT Bovine alphaherpesviruses 1 and 5 (BoHV-1/5) are main pathogens of respiratory, reproductive and neurological diseases in cattle. The aim of this study was to investigate the frequency of neutralizing antibodies against BoHV-1/5 in serum samples and to detect viral DNA in semen of bulls from beef cattle farms located in RS. A total of 372 serum and semen sample from bulls were collected in eighteen farms. Serum samples were submitted to virus neutralization (VN) assay, while semen samples were used to detect BoHV-1 and BoHV-5 DNA by PCR. VN results showed that BoHV-1/5 antibodies were detected in bulls of 66.7% (12/18) of the farms, 295 (79.5%) BoHV positive bulls, 287 for BoHV-1 and 234 for BoHV-; at 43 vaccinated bulls 72.1% (31/43) showing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BoHV-1 and 5; Co-infection; DNA; Semen. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100197 |
| |
|
|
| Neves,M.M.; Heneine,L.G.D.; Henry,M.. |
| The aim of this study was to evaluate the use of low concentrations of natural and lyophilized low density lipoprotein (LDL) from hen's egg yolk for cryopreservation of canine semen. Different ammonium sulphate concentrations were tested to extract LDL from egg yolk. The yolk was centrifuged, and LDL was isolated using 10, 20, 40, 45, or 50% ammonium sulphate solution (ASS). The LDL-rich floating fraction was collected for chemical characterization. Dry matter content was lowest (P<0.05) in the LDL extracted with the 50% ASS. The purification of LDL increased in association with increasing ammonium sulphate concentrations. SDS-PAGE showed that the 50% ASS solution yielded a purer fraction of LDL from egg yolk. For semen cryopreservation, TRIS extender... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Egg Yolk; LDL extraction; Ammonium sulphate; Dog; Semen. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300769 |
| |
|
|
| Silva,A.R.; Cardoso,R.C.S.; Silva,L.D.M.. |
| Standardized sperm concentration and volume:volume extension were compared as dilution rates for canine semen freezing. Six proven stud dogs were submitted to two seminal collections by manual stimulation. Semen was evaluated and extended in tris plus egg-yolk and glycerol according to two different dilution rates. The first one was based on a standardized sperm concentration of 200x10(6) spermatozoa/ml and the second was a volume:volume extension at a proportion of one part semen to one part extender. Semen was frozen, stored in liquid nitrogen and thawed after one week. Sperm motility and vigor were appraised after each stage of the process and at 15 and 30min post-thawing. Sperm morphology was analyzed after collection and thawing. No differences were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Semen; Extender; Freezing; Dilution. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600010 |
| |
| Registros recuperados: 65 | |
|
|
|
