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| Registros recuperados: 35 | |
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| Vieira, Viviane Cristina; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista; Alves, Pedro Luís da Costa Aguiar; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista; Picchi, Simone Cristina; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista; Lemos, Manoel Victor Franco; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista; Sena, Janete Apparecida Desidério; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista. |
| Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to... |
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Palavras-chave: Digitaria; PsbA gene; RAPD; Sequencing; Triazine; Weed Digitaria; PsbA gene; RAPD; Sequencing; Triazine; Weed. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841 |
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| Deef, Lamiaa Elsayed Mokhtar. |
| This is the first study to detection the genetic relationship between Porphyrio alleni and four Rallidae species: Fulica atra, Gallinulla angulata, Gallinulla chloropus and Porphyrio porphyrio. Mitochondrial cytochrome oxidase subunit I (COI) sequences were used as an effective marker in this study. DNA of Rallidae species were extracted, amplified using polymerase chain reaction (PCR) and then sequenced. The results obtained from information based on COI sequences revealed that Gallinulla angulata and Gallinulla chloropus fall into two separate clades and they are not monophyletic. This suggests that, two moorhens could not be laid into the same genus. In addition, Porphyrio porphyrio was included in the same genus with Porphyrio alleni but they were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Birds; Gruiformes; PCR; Phylogeny; Sequencing; Species.. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/37184 |
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| SANTOS,LIDIANE F. DOS; LANA,ROGÉRIO P.; SILVA,MARLIANE C.S. DA; VELOSO,TOMÁS G.R.; KASUYA,MARIA CATARINA M.; RIBEIRO,KARINA G.. |
| Abstract Effective microorganisms (EM) are inoculants formed by fungi and bacteria isolated from soil. EM are commonly used by farmers on agronomic crops to stimulate plant growth, but their composition and their benefits has been controverted. This study aimed to analyze the diversity of microorganisms growing in three EM inoculants, as well as to evaluate their efficiency in the germination of palisade grass seeds. The total DNA of the three EM inoculants was extracted, the 16S rRNA and ITS genes were amplified by PCR and sequenced on the Illumina MiSeq platform. Germination tests were conducted with three type of the EM, in three concentration and two times of the immersion. The bacterial group was the most abundant in EM, followed by fungi. Bacterial... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diversity; Dormancy; Sequencing; Sulfuric acid. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000201002 |
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| Viancelli,Aline; Kunz,Airton; Esteves,Paulo Augusto; Bauermann,Fernando Viçosa; Furukawa,Kenji; Fujii,Takao; Antônio,Regina Vasconcellos; Vanotti,Matias. |
| The present study aimed to describe the bacterial community present at an anaerobic up flow bioreactor with ANAMMOX activity, inoculated with the sludge from the anaerobic pond of a swine slurry treatment system. The description was based on the molecular DNA techniques using primers for amplification of complete 16S rRNA gene and also new primers to amplify smaller fragments from 16S rRNA. During the bioreactor operation time, the bacterial community changed significantly, increasing the nitrogen removal efficiency, reaching after 500 days a removal rate of 94%. The complete PCR amplification of 16S rRNA gene generated 17 clones, where three presented similarity with Candidatus Jettenia asiatica (97%), twelve with Janthinobacterium (99%) and two with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ANAMMOX; Anaerobic pond; Sequencing; Swine manure. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500022 |
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| Toyoshima,Marcos Tadashi Kakitani; Keller,Lilian Walsh; Barbosa,Maria Luisa; Durigon,Edison Luiz. |
| Papular-purpuric "gloves and socks" syndrome (PPGSS) is a novel, rare, self-limiting dermatosis caused by human parvovirus B19. It consists of pruritic edema and erythema of the hands and feet in a gloves-and-socks distribution, and it is associated with oral lesions and fever. We present a case of PPGSS in a 22-year-old Brazilian woman. Clinical and laboratory evaluation, including serological tests, PCR and gene sequencing, confirmed the presence of human parvovirus B19. |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Dermatosis; Gloves and socks; Parvovirus B19; PCR; Sequencing. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000100013 |
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| Chudobova,Dagmar; Cihalova,Kristyna; Guran,Roman; Dostalova,Simona; Smerkova,Kristyna; Vesely,Radek; Gumulec,Jaromir; Masarik,Michal; Heger,Zbynek; Adam,Vojtech; Kizek,Rene. |
| ABSTRACT BACKGROUND: Infections, mostly those associated with colonization of wound by different pathogenic microorganisms, are one of the most serious health complications during a medical treatment. Therefore, this study is focused on the isolation, characterization, and identification of microorganisms prevalent in superficial wounds of patients (n = 50) presenting with bacterial infection. METHODS: After successful cultivation, bacteria were processed and analyzed. Initially the identification of the strains was performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based on comparison of protein profiles (2-30 kDa) with database. Subsequently, bacterial strains from infected wounds were identified by both... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial strains; MALDI-TOF; Sequencing; Superficial wounds. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600604 |
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| Miagostovich,M.P.; Santos,F.B. dos; Simone,T.S. de; Costa,E.V.; Filippis,A.M.B.; Schatzmayr,H.G.; Nogueira,R.M.R.. |
| The genetic characterization of dengue virus type 3 (DEN-3) strains isolated from autochthonous cases in the State of Rio de Janeiro, Brazil, in 2001 is presented. Restriction site-specific (RSS)-PCR performed on 22 strains classified the Brazilian DEN-3 viruses as subtype C, a subtype that contains viruses from Sri Lanka, India, Africa and recent isolates from Central America. Nucleic acid sequencing (positions 278 to 2550) of one DEN-3 strain confirmed the origin of these strains, since genotype III - classified by sequencing - and RSS-PCR subtype C are correlated. This genetic subtype has been associated with hemorrhagic dengue epidemics and the information provided here could be useful to implement appropriate prevention and control measures. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Dengue viruses type 3; Sequencing; RSS-PCR; Rio de Janeiro; Brazil. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800002 |
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| Lilenbaum,Walter; Kremer,Frederico; Ristow,Paula; Dellagostin,Odir; Bourhy,Pascale; Hartskeerl,Rudy; Vasconcellos,Silvio. |
| Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira; Serogrouping; Sequencing; PFGE; Rrs. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050 |
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| Fernandes,Maria Judite Bittencourt; Simoni,Isabela Cristina; Harakava,Ricardo; Rivas,Eliana Borges; Arns,Clarice Weis. |
| Infectious bursal disease virus (IBDV) is classified according to the antigenicity and virulence into classical virulent (cv), very virulent (vv), and antigenic variant strains. The molecular basis for the IBDV antigenic variation is well established and is associated to the capsid protein, VP2 (gene VP2 of segment A), whereas both VP2 and the RNA-dependent RNA polymerase, VP1 (gene VP1 of segment B), have been correlated with the virulence. In this study, seventeen Brazilian IBDV samples previously characterized by the VP2 gene as cv (three) and vv (fourteen) strains were genetically and molecularly analyzed for their VP1 gene. All of the strains kept with the same cv or vv classification except one sample, Br/03/DR. This sample was classified as vv by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian infectious bursal disease virus; Very virulent strain; VP1; Sequencing; Phylogenetic analysis. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300024 |
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| Hernández Deheza, María Guadalupe. |
| En Yurécuaro, Michoacán, en el ciclo de cultivo verano otoño 2009 y 2011 se encontraron en cultivos de chile síntomas de variegado, nervaduras cloróticas, deformación de la lámina foliar y fruto, albinismo y escaldadura, síndrome al cual se le denominó “variegado del chile”. A simple vista, esta serie de síntomas indicaban que el problema era causado por virus fitopatógenos; sin embargo, en ensayos previos se detectó a Candidatus Liberibacter solanacearum, por lo que el objetivo del presente trabajo fue determinar si esta bacteria es el agente causal del variegado del chile. Mediante transmisión por injerto, transmisión con el vector Bactericera cockerelli y reacción en cadena de la polimerasa con el par de iniciadores OA2/CLi.po.F y OI2, se confirmo que... |
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Palavras-chave: Candidatus Liberibacter; PCR; Secuenciación; Sequencing; Entomología y Acarología; Doctorado. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/1844 |
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| Chibeu,Andrew; Lavigne,Rob; Mattheus,Wesley; Matthijs,Sandra; Cornelis,Pierre; Volckaert,Guido. |
| We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin/streptomycin/chloramphenicol-resistant cosmid vector. pRG930cm (17,256 bp; GenBank Accession No.: FM174471) has a broad host range, and is stably maintained by a number of Gram-negative bacteria including Pseudomonas spp, Escherichia coli, Agrobacterium tumefaciens and Azorhizobium caulinodans ORS571. pRG930cm is already widely used and its sequence will aid efficient construction and analysis of cosmid libraries. |
| Tipo: Journal article |
Palavras-chave: Cosmid; PRG930cm; Pseudomonas; Sequencing. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200010 |
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| Registros recuperados: 35 | |
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