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Anti-Trypanosoma cruzi antibody detection in blood donors in the Southern Brazil BJID
Araújo,A.B.; Vianna,E.E.S.; Berne,M.E.A..
Trypanosoma cruzi, the causal agent of Chagas' Disease, is a widely spread protozoa in America. Blood transfusion is the secondly most important way of acquiring the infection. In blood banks, tests are performed to eliminate potentially infected blood. This study aimed to evaluate the positivity for T. cruzi in blood samples of donor's candidates in Southern Brazil. The study was based on a sampling containing all blood donors of Hemopel - a Pelotas City Blood Center, Rio Grande do Sul State, Brazil, from 2004 to 2005. Serological study was performed using ELISA Chagatest. Sampling containing values ± 20% cut off were evaluated using ELISA Chagatek, ELISA Alka/Adaltis, IHA Chagatest and IIF Imunocruzi. TESA-Blot was used as a confirmatory procedure in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosoma cruzi; Blood donors; Serological screening.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600007
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SEROLOGICAL SCREENING FOR INFECTIOUS CATTLE DISEASES: II. ASSOCIATION BETWEEN PREVALENCE AND LEVEL OF ELISA RESPONSE Ciência Rural
Rojas,José Alfonso Barajas-; Riemann,Hans; Franti,Charles.
A herd of cattle (Holstein-Zebu crosses) was screened every two months by ELISA during a period of two years for IgG antibodies against 19 infectious disease agents. Two hundred and ninety five sera were collected from 157 young animals (0-4 months of age), 1037 sera from 292 developing animals (4-36 months of age) and 1468 sera from 259 producing animals (> 36 months of age). The results indicate that the difference in ELISA between positive and negative tests is associated with the overall prevalence of positive tests. When the prevalence of positive tests is low the difference between positive and negative tests is greater than when the prevalence is intermediate or high. This means that ELISA, presumably other serological tests for IgG antibodies,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Serological screening; Cattie diseases; ELISA.
Ano: 1993 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000200014
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SEROLOGICAL SCREENING FOR INFECTIOUS CATTLE DISEASES: I. INFLUENCE OF REPRODUCTIVE STATUS Ciência Rural
Barajas-Rojas,Jose Alfonso; Riemann,Hans; Franti,Charles.
A cohort of 125 cows (Zebu-Holstein crosses) were screened every two months during a period of two years for IgG antibodies to 20 viral, bacterial, rickettsial and parasitic agents. The screening was performed on sera using the ELISA procedure. The IgG levels fluctuated during pregnancy in a regular and similar way for all the antigens tested. IgG reached its lowest levels in the fourth and seventh month of pregnancy, a significant increase ocurred during the last month of pregnancy and this was followed by a precipitous decline during the first month post partum. The IgG fluctuations associated with the different phases of reproductive status may interact with seasonal changes in IgG levels. The changes in IgG levels during pregnancy may have the result...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cohort; Serological screening; Pregnancy.
Ano: 1993 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000100013
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