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Seroprevalence for brucellosis and leptospirosis in dogs from Belém and Castanhal, State of Pará, Brazil Acta Amazonica
PAZ,Giselle Souza da; ROCHA,Katarine de Souza; LIMA,Michele de Souza; JORGE,Ediene Moura; PANTOJA,José Carlos Figueiredo; MORAES,Carla Cristina Guimarães de; LANGONI,Helio.
Brucellosis and leptospirosis are widely spread bacterial infections and dogs are the most important source of infection and reservoir for diseases. Dogs can disseminate the agents in the environment and transmit them to humans and/or other animals. The objective of this study was assess the occurrence of reactive to antibodies anti-Leptospira spp., Brucella canis and B. abortus in Belém and Castanhal, State of Pará, Amazon, Brazil. A total of 156 samples were randomly collected in the city of Belém and 158 samples in Castanhal. The anti-B. canis antibodies research was performed by Agar Gel Immunodiffusion (AGID) with and without 2-mercaptoethanol serum treatment (AGID-2ME). To assess the anti-B. abortus antibodies, the technique of Fast Seroagglutination...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospira spp.; Brucella spp.; Domestic canids; Serology.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672015000300265
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DETECTION OF INFECTION WITH Toxoplasma gondii IN MANATEES (Trichechus inunguis) OF THE PERUVIAN AMAZON Acta biol.Colomb.
DELGADO,PATRICK MATHEWS; PEREA,NOFRE SANCHEZ; MATHEWS DELGADO,JONH PAUL; GARCIA,CLAUDIA BIFFI; MALHEIROS,ANTONIO FRANCISCO; GARCIA DAVILA,CARMEN ROSA.
The Amazonian manatee (Trichechus inunguis) is an aquatic mammal that inhabits freshwater environments and is endemic to the Amazon Basin. The presence of Toxoplasma gondii antibodies was investigated in 19 manatees in one rescue unit in the northern region of Peru. Antibodies to T. gondii were detected in 12 (63.2 %) of 19 animals by using the modified agglutination test (titer, 1:25), and no association between sex and age of the animals and the presence of T. gondii antibodies was observed (p < 0.05). The results suggest a contamination by T. gondii oocysts in the aquatic environment where these animals live.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazonian manatee; Peruvian Amazon; Serology; Toxoplasma gondii.
Ano: 2013 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2013000100017
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Antibody response between pigs of Piau and a commercial breed naturally infected with Porcine circovirus 2 Arq. Bras. Med. Vet. Zootec.
Bulos,L.H.S.; Gonzaga,N.F.; Souza,L.F.L.; Assao,V.S.; Leite,R.A.S.; Rebouças,M.S.; Scatamburlo,T.M.; Guimarães,J.D. Guimarães S.E.F.; Lopes,P.S.; Almeida,M.R.L.; Silva Júnior,A..
ABSTRACT Brazilian pig population is made up of several naturalized breeds; among them the Piau breed is known for its rusticity and large fat stores. The naturalized breeds, in comparison with commercial ones, may have an increased resistance to diseases circulating in their territory. Thus, this study aimed to verify if there are differences between the serologic profile against Porcine circovirus 2 (PCV2) of Piau pigs and that of a commercial breed from a farm naturally infected by PCV2. The serum viral load was measured by qPCR, and levels of anti-PCV2 antibodies were measured by ELISA. The results showed that the serum viral load was similar across all animals. However, Piau piglets showed higher levels of antibodies compared to commercial piglets (P=...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Serum viral load; Serology; Piau.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501145
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Serum neutralization as a differential serological test for classical swine fever virus and other pestivirus infections Arq. Bras. Med. Vet. Zootec.
Paredes,J.C.M.; Oliveira,E.A.S.; Oliveira,L.G.; Rosa,J.C.A.; Roehe,P.M..
Serum neutralization tests (SN) were performed against classical swine fever virus (CSFV), bovine viral diarrhea virus (BVDV) and border disease virus (BDV) on samples of swine serum collected for screening of antibodies to CSFV, in order to determine the SN value as a differential serological test. Ninety-nine sera out of a sample of 16,664 were positive for antibodies to pestiviruses in an ELISA test which did not distinguish antibodies to different pestiviruses. When submitted to SN, 81 sera were positive for CSFV antibodies only. In 17 sera, crossreactive antibodies to either CSFV, BVDV or BDV were detected. In most of these sera (13 out of 17) the differences between SN titres against the three viruses were not sufficient to estimate which was the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Swine; Classical swine fever; Pestivirus; Serology.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000500001
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First molecular and serological detection of Epizootic Hemorrhagic Disease virus in white tailed deer ( Odocoileus virginianus ) from Tamaulipas, Mexico Arq. Bras. Med. Vet. Zootec.
Merino,J.O.; Cruz,NI. De la; Galvan,G.; León,A.P. De; Burnes,J..
ABSTRACT Epizootic hemorrhagic disease viruses (EHDV) are dsRNA arboviruses transmitted by biting midges of the genus Culicoides that cause disease in domestic and wild ruminants. Epizootic hemorrhagic disease (EHD) is considered the most important infectious disease of white tailed deer (WTD) in North America, some studies in Northeast Mexico reported EHDV-seropositive WTD and EHDV-infected Culicoides vectors. The increasing population of WTD that share habitat with livestock in Northeast México highlights the importance of EHD for the livestock industry in the transboundary region with the U.S. One hundred and twenty two samples from WTD in Tamaulipas state, Mexico were tested by ELISA and RT-PCR for EHDV antibodies and nucleic acid, respectively. Twelve...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Deer; Detection; EHDV; RT-PCR; Serology.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100077
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Epidemiological assessment of canine brucellosis Arq. Bras. Med. Vet. Zootec.
Megid,J.; Brito,A.F.; Moraes,C.C.G.; Fava,N.; Agottani,J..
Observou-se brucelose canina em quatro canis diferentes. Os canis apresentaram animais com história de aborto, mortalidade em neonatos e nascimentos prematuros. A porcentagem de animais soropositivos para brucelose canina, pela prova de imunodifusão em ágar gel, variou de 4,6 a 57,1%. Observou-se correlação positiva entre porcentagem de animais positivos e aspectos reprodutivos e condições de aglomeração.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Dog; Brucellosis; Epidemiology; Serology.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000500007
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Development and standardization of a western blotting test for detection of antibodies against B. abortus Arq. Bras. Med. Vet. Zootec.
Falcão,M.V.D.; Santana,V.L.A.; Corrêa,F.N.; Tenório,J.A. B.; Mota,R.A..
ABSTRACT Brucellosis is an infectious disease caused by bacteria of the genus Brucella spp. with diagnosis based on use of serological techniques. The present study aimed to develop and standardize a western blotting (WB) test for detection of antibodies against B. abortus. Samples from two groups of cattle were analyzed: group I: 60 serum samples from true positive and true negative vaccinated animals (30 positive samples from infected animals according to rose bengal test (RBT), 2-mercaptoethanol, serum agglutination test (SAT) and complement fixation test (CFT) and 30 RBT negatives samples); group II: 383 field samples (90 positive and 293 CFT negative sera). The most reactive band in the western blotting, which properly identified and separated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brucellosis; Immunodiagnosis; Proteomics; Serology.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100160
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A comparison of two agar gel immunodiffusion methods and a complement fixation test for serologic diagnosis of Brucella ovis infection in experimentally infected rams Arq. Bras. Med. Vet. Zootec.
Xavier,M.N.; Sant'Anna,F.M.; Silva,T.M.A.; Costa,E.A.; Moustacas,V.S.; Merlo,F.A.; Carvalho Júnior,C.A.; Dasso,M.G.; Mathias,L.A.; Gouveia,A.M.G.; Lage,A.P.; Santos,R.L..
A infecção por Brucella ovis é considerada uma das principais causas de epididimite e infertilidade em carneiros, resultando em falhas reprodutivas e perdas econômicas significativas em rebanhos ovinos ao redor do mundo. O estudo teve o objetivo de avaliar três testes sorológicos disponíveis para o diagnóstico da brucelose ovina por B. ovis, utilizando 181 soros ovinos. Amostras de soro provenientes de carneiros experimentalmente infectados foram coletadas ao longo de 192 dias pós-infecção (n=117) e durante o período pré-infecção (n=9). Adicionalmente, amostras de soro foram obtidas de ovinos provenientes de um rebanho livre para B. ovis (n=55). As técnicas de imunodifusão em gel de agar (IDGA), utilizando dois antígenos disponíveis comercialmente, e de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ram; Brucella ovis; Serology; Complement fixation; Immunodiffusion.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400031
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Natural infection of Viola cornuta (Violaceae) with Cucumber mosaic virus, subgroup I Biocell
Arneodo,Joel; de Breuil,Soledad; Lenardon,Sergio; Conci,Luis.
Plants of Viola cornuta displaying typical virus symptoms were observed during spring 2003 in a plant nursery in Córdoba, central Argentina. Electron microscopic examinations of symptomatic leaf samples revealed the presence of isometric virus-like particles about 30 nm in diameter. Subsequent serological analysis allowed the identification of the pathogen as a subgroup I strain of Cucumber mosaic virus (CMV). These results were confirmed by antigen capture - reverse transcription - polymerase chain reaction with specific CMV primers, and digestion with a restriction enzyme. This is the first report of CMV infecting V. cornuta in Argentina.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Viola cornuta; Cucumber mosaic virus; Electron microscopy; Serology; AC-RT-PCR.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200008
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Exposure of pampas fox (Pseudalopex gymnocercus) and crab-eating fox (Cerdocyon thous) from the Southern region of Brazil to Canine distemper virus (CDV), Canine parvovirus (CPV) and Canine coronavirus (CCoV) BABT
Hübner,Silvia de Oliveira; Pappen,Felipe Geraldes; Ruas,Jerônimo Lopes; Vargas,Gilberto D'Ávila; Fischer,Geferson; Vidor,Telmo.
The exposure of 13 Brazilian free-ranging nondomestic canids (five pampas fox - Pseudalopex gymnocercus and eight crab-eating fox -Cerdocyon thous) from Southern region of Brazil, to Canine distemper virus (CDV), canine parvovirus (CPV) and Canine coronavirus (CCoV) was investigated. Antibodies against CDV were detected in 38.5% (5/13) of the samples. There were anti-CDV antibodies in 60% (3/5) of P. gymnocercus and in 25% (2/8) of C. thous. The frequency was higher among the adults and males. Eleven canids (84.6%) presented antibodies against CPV, 80% (4/5) were from P. gymnocercus and 87.5% (7/8) were from C. thous. There was no difference in positivity rate against CPV between gender and age. Antibodies against CCoV were detected in 38.5% (5/13) of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudalopex gymnocercus; Cerdocyon thous; Canine distemper virus (CDV); Canine parvovirus (CPV); Canine coronavirus (CCoV); Serology.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300012
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Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4 BABT
Krüger,Ernesto Renato; Penha,Tânia Regina; Hummelgen,Fernanda Cristina; Agottani,Jorge Bacila; Reva,Demétrio; Gonçalves,Robson; Thomaz-Soccol,Vanete.
ABSTRACTThe aim of this work was to develop and evaluate an indirect enzyme-linked immunosorbent assay (ELISA) and made a serological screening for specific antibodies to BoHV-4. Bovine serum samples were collected from different Brazilian states and evaluated for the presence of antibodies for BoHV-4 and BoHV-1. The serological results obtained showed that the indirect ELISA assay could be applied for the detection of specific antibodies for BoHV-4. The ELISA test allowed concluding that BoHV-4 is present in bovines in every Brazilian state from which serum samples were collected. The ELISA assay here standardized proved to be useful for the epidemiological studies and showed a positivity range from 1.8 to 66% in Brazil.
Tipo: Info:eu-repo/semantics/article Palavras-chave: BoHV-4; Serology; Diagnostic; Cattle; Epidemiology.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500725
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Latent human herpesvirus - 8 (HHV-8) infection in female commercial sex workers from Imbituba, Santa Catarina, Brazil BJID
Caterino-de-Araujo,Adele; Santos-Fortuna,Elizabeth; Magri,Mariana Cavalheiro; Schuelter-Trevisol,Fabiana; Silva,Marcos Vinicius.
Human herpesvirus 8 (HHV-8) infection was identified in 6 out of 90 (6.7%) female commercial sex workers from Imbituba, Santa Catarina, and was associated to age. Frequencies of 5.6% of anti-latent and 3.3% of anti-lytic antibodies were detected. Considering non-endemic areas from Brazil, the anti-latent antibodies frequency seems elevated and requires further investigation on referent female population.
Tipo: Info:eu-repo/semantics/other Palavras-chave: HHV-8; Serology; Anti-latent antibodies; Female sex workers.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100004
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Bartonella native valve endocarditis: the first brazilian case alive and well BJID
Lamas,C.; Favacho,A.; Ramos,R.G.; Santos,M.S.; Ferravoli,G.I.; Weksler,C.; Rozental,T.; Bóia,M.N.; Lemos,E.R.S..
Bartonella is an important cause of blood culture-negative endocarditis in recent studies. Seroprevalence studies in the States of Minas Gerais and Rio de Janeiro have shown Bartonella IgG positivity around 14% in healthy adults and 40% in HIV seropositive adults, respectively. A case report of a 46-year-old white male with moderate aortic regurgitation (AR) due to rheumatic heart disease (RHD), admitted due to worsening heart failure, is presented. Clinical features were apyrexia, anemia, polyclonal hypergammaglobulinemia, hematuria and splenomegaly. He was submitted to surgery due to worsening AR. Histopathology of the excised valve showed active bacterial endocarditis and underlying RHD. Routine blood cultures were negative. Indirect immunofluorescence...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bartonella spp.; Infective endocarditis; Blood culture-negative infective endocarditis; Rheumatic heart disease; Serology; Indirect immunofluorescence.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000600012
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First survey for detecting the presence of human herpesvirus 8 infection (HHV-8) in Maputo, Mozambique BJID
Caterino-de-Araujo,Adele; Manuel,Rolanda Carmen Rafael; Bianco,Rosana Del; Santos-Fortuna,Elizabeth; Magri,Mariana Cavalheiro; Silva,Joyce Matie Kinoshita; Bastos,Rui.
Human herpesvirus 8 (HHV-8), the etiological agent of Kaposi's sarcoma (KS), is endemic in parts of the sub-Saharan, and KS has increased concomitantly with the HIV/AIDS epidemic. In Mozambique (MZ), no data concerning HHV-8 infection was available, thus the main of this work was to investigate, for the first time, the presence of HHV-8 infection in Maputo, MZ. Latent and lytic HHV-8-specific antibodies were assessed in blood samples from 189 individuals from the Central Hospital of Maputo, MZ, using "in-house" immunofluorescence assays conducted in São Paulo, Brazil. The results obtained were analyzed according to socio-demographic and clinical variables using the Chi-square test and logistic regression. An HHV-8 seropositivity of 1.8% and 9.7% was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human herpesvirus 8 (HHV-8); Serology; Mozambique; Frequency; Kaposi's sarcoma.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300008
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Dengue: a review of the laboratory tests a clinician must know to achieve a correct diagnosis BJID
De Paula,Sérgio Oliveira; Fonseca,Benedito Antônio Lopes da.
Dengue is the most important disease caused by an arbovirus (1, 2, 3 and 4 serotypes) worldwide, especially in the tropical and sub-tropical regions. Its clinical manifestations range from asymptomatic infections to a severe disease characterized by hemorrhage and shock. The incidence of dengue virus activity in the Americas has substantially increased from 1980 to 1994. In Brazil, the increase in the incidence of dengue is especially linked to the dissemination of Aedes aegypti. Thus, a rapid and accurate dengue diagnosis is of paramount importance for effective control of dengue outbreaks [8]. Five serological tests have been used for the diagnosis of dengue infection: hemagglutination-inhibition (HI), complement fixation (CF), neutralization test (NT),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dengue; Diagnosis; Serology; PCR.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000600002
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Neurobrucellosis: clinical, diagnostic, therapeutic features and outcome. Unusual clinical presentations in an endemic region BJID
Ceran,Nurgul; Turkoglu,Recai; Erdem,Ilknur; Inan,Asuman; Engin,Derya; Tireli,Hulya; Goktas,Pasa.
Brucellosis is a zoonotic infection and has endemic characteristics. Neurobrucellosis is an uncommon complication of this infection. The aim of this study was to present unusual clinical manifestations and to discuss the management and outcome of a series of 18 neurobrucellosis cases. Initial clinical manifestations consist of pseudotumor cerebri in one case, white matter lesions and demyelinating syndrome in three cases, intracranial granuloma in one case, transverse myelitis in two cases, sagittal sinus thrombosis in one case, spinal arachnoiditis in one case, intracranial vasculitis in one case, in addition to meningitis in all cases. Eleven patients were male and seven were female. The most prevalent symptoms were headache (83%) and fever (44%). All...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neurobrucellosis; Serology; Diagnosis; Neuroimaging.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100010
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Genital schistosomiasis mansoni concomitant to genital tumor in areas of low endemicity: challenging diagnosis BJID
Cavalcanti,Marta G; Gonçalves,Margareth MI; Barreto,Magali M; Silva,Aline Helen da; Madi,Kalil; Peralta,José Mauro; Igreja,Ricardo P.
Genital infection by Schistosoma mansoni is usually misdiagnosed in individuals who reside in, or travel to endemic areas. We describe two cases of genital tumor associated with S. mansoni infection manifested by methrorragy. Surgical specimens revealed leiomyomas in both cases associated with S. mansoni. In one of them, granulomas were found in the ovary and in the other they were found in the uterine tube. Although none presented intestinal/hepatic disease, fecal egg excretion was detected in one. Both had elevated pretreatment antibody reactivity to S. mansoni antigen, but follow-up showed different outcomes. Schistosomiasis should be considered as a diagnosis in individuals with methrorragy residing in or having traveled to endemic areas. Since...
Tipo: Info:eu-repo/semantics/article Palavras-chave: S. mansoni; Female genital infection; Serology; Low endemic area.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200016
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Clinical significance of different bacterial load ofMycoplasma pneumoniae in patients with Mycoplasma pneumoniae pneumonia BJID
Jiang,Wujun; Yan,Yongdong; Ji,Wei; Wang,Yuqing; Chen,Zhengrong.
OBJECTIVE:This retrospective study was conducted to investigate the clinical significance of differentMycoplasma pneumoniae bacterial load in patients with M. pneumoniae pneumonia (MP) in children.METHODS:Patients with MP (n=511) were identified at the Children's Hospital Affiliated to Soochow University database during an outbreak of MP between January 2012 and February 2013.RESULTS:Comparing patients with high and low bacterial load those with higher loads were significantly older (p<0.01) and had fever significantly more frequently (p=0.01). Presence of wheezing at presentation was associated with low bacterial load (p=0.03). Baseline positive IgM was present in 93 (56.4%) patients with high bacterial load compared to 46 (27.8%) patients with low...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma pneumoniae; Pneumonia; Polymerase chain reaction; Serology.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000200124
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Experimental infection with Brazilian Newcastle disease virus strain in pigeons and chickens BJM
Carrasco,Adriano de Oliveira Torres; Seki,Meire Christina; Benevenute,Jyan Lucas; Ikeda,Priscila; Pinto,Aramis Augusto.
Abstract This study was designed with the goal of adding as much information as possible about the role of pigeons (Columba livia) and chickens (Gallus gallus) in Newcastle disease virus epidemiology. These species were submitted to direct experimental infection with Newcastle disease virus to evaluate interspecies transmission and virus-host relationships. The results obtained in four experimental models were analyzed by hemagglutination inhibition and reverse transcriptase polymerase chain reaction for detection of virus shedding. These techniques revealed that both avian species, when previously immunized with a low pathogenic Newcastle disease virus strain (LaSota), developed high antibody titers that significantly reduced virus shedding after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gallus gallus; Columba livia; Experimental infection; RT-PCR; Serology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100231
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Mycoplasma synoviae infection on Newcastle disease vaccination of chickens BJM
Silva,Rita de Cássia Figueira; Nascimento,Elmiro Rosendo do; Pereira,Virgínia Léo de Almeida; Barreto,Maria Lúcia; Nascimento,Maria da Graça Fichel do.
Newcastle disease is characterized by respiratory manifestations in association with nervous and/or digestive symptoms. Its prevention is done by vaccination with live attenuated (lentogenic strains) and/or killed vaccines. The lentogenic strains can lead to strong post-vaccination reaction, principally due to the presence of other pathogenic agents. Among them, Mycoplasma synoviae is worldwide important, mainly in Brazil. The dissemination of this agent in poultry flocks has been achieved due to difficulties in diagnosis and disease reproduction, virulence variations among different M.synoviae strains, and attribution of typical M.synoviae disease manifestation to other disease agents. This experimental study in SPF chicks (Gallus gallus), previously...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vaccine; Serology; Mycoplasma; Newcastle disease.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200033
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