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| Vasconcellos,Silvio A.; Oliveira,João C.F.; Morais,Zenáide M.; Baruselli,Pietro S.; Amaral,Renato; Pinheiro,Sônia R.; Ferreira,Fernando; Ferreira-Neto,José S.; Schönberg,Arno; Hartskeerl,Rudy A.. |
| In April 1998 urine samples from adult female buffaloes were collected in a farm located in Registro, Vale do Ribeira, São Paulo State, Brazil. The urine samples obtained after furosemide injection were immediately transported to the laboratory in liquid modified EMJH medium and seeded, by the serial dilution technique, into Fletcher's or modified EMJH-0.2% agar, both of them with 5-fluorouracil 100mg/mL. The intraperitoneoum inoculation of 0.5 mL was also performed with each urine sample in young, adult hamsters (Mesocricetus auratus). All samples seeded directly in culture medium were contaminated. The hamsters did not show any sign of disease and were killed at the 21st post inoculation day. At this time kidney cultures of these animals were performed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; Serogroup Sejroe; Serovar guaricura; Buffalo. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400008 |
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