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Hepatitis C virus detection in the semen of infected patients BJID
Cavalheiro,Norma de Paula; Santos,Ana Carolina de Oliveira; Melo,Carlos Eduardo; Morimitsu,Suzana Rie; Barone,Antonio Alci.
Though HCV infection is a serious public health problem, some aspects of its biology are still not well understood, such as its transmission through seminal fluid and sexual transmission. We looked for HCV in the semen of infected patients. Thirteen patients were included. Semen fractions (seminal plasma, leukocytes and spermatozoa) were separated with 45% and 90% Percoll gradients. The HCV-RNA in blood and semen fractions was extracted using the same protocol (AMPLICOR Roche) and was detected using the qualitative Roche Amplicor test and by agarose gel electrophoresis, with ethidium bromide staining. The mean age of the patients was 40.7 years. Risk factors for the acquisition of HCV included injectable and inhaled drug use in six (42.8%), blood...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HCV; PCR; Sexual transmission; Semen.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500003
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Two variants of HIV-1 B serotype are transmitted heterosexually in São Paulo, Brazil BJMBR
Casseb,J.; Hong,M.A.; Gonsalez,C.; Brígido,L.F.; Duarte,A.J.S.; Michael-Hendry,R..
HIV-1 variability may have an important impact on transmission and pathogenicity. Better characterization of the HIV epidemic in Brazil is necessary for the development of vaccine trials in this country. We analyzed sera from 108 HIV-1-infected volunteers from São Paulo City to determine serotype and reactivity for V3 motifs of HIV in this population, and the relationship to transmission mode. We concluded that the HIV-1 B serotype is frequent among heterosexually infected women, even in the absence of anal sex, and that two major V3 motifs, GPGR and GWGR, had similar prevalence among women (48% and 52%, respectively) and men (56% and 44%, respectively). We also observed an equal distribution of these strains regardless of their CD4+ T cell counts,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: HIV; Serotyping; Gp 120 V3 loop; Synthetic peptides; Sexual transmission.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000002
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Transmission routes of visceral leishmaniasis in mammals Ciência Rural
Oliveira,Vinícius Vasconcelos Gomes de; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da.
Visceral leishmaniasis (VL) is a chronic disease caused by Leishmania infantum. The major sites of parasite localization in infected animals are the secondary lymphoid organs, bone marrow and cutaneous tissue. However, reports exist on the detection of the parasite in the organs of the male and female genital system. The main route of transmission is related to the hematophagous sandfly vectors of the genus Lutzomyia(New World) and Phlebotomus(Old World). However, other routes of transmission may be mentioned, such as sexual, vertical, hematogenic without vector and others involved in VL epidemiology. Thus, the current article reviews the main forms of transmission of visceral leishmaniasis in mammals
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmania sp; Dogs; Sexual transmission; Vertical transmission.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000901622
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