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| Yamamoto,D.; Coimbra,V.C.; Okuda,K.; Rabinovitch,M.. |
| Invasive bacteria can induce their own uptake and specify their intracellular localization; hence it is commonly assumed that proximate modulation of host cell transcription is not required for infection. However, bacteria can also modulate, directly or indirectly, the transcription of many host cell genes, whose role in the infection may be difficult to determine by global gene expression. Is the host cell nucleus proximately required for intracellular infection and, if so, for which pathogens and at what stages of infection? Enucleated cells were previously infected with Toxoplasma gondii, Chlamydia psittaci, C. trachomatis, or Rickettsia prowazekii. We enucleated L929 mouse fibroblasts by centrifugation in the presence of cytochalasin B, and compared... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shigella flexneri; Cell enucleation; Cytoplasts; L929 fibroblasts. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600007 |
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| Penatti,M.P.A.; Hollanda,L.M.; Nakazato,G.; Campos,T.A.; Lancellotti,M.; Angellini,M.; Brocchi,M.; Rocha,M.M.M.; Silveira,W. Dias da. |
| Shigella spp are Gram-negative, anaerobic facultative, non-motile, and non-sporulated bacilli of the Enterobacteriaceae family responsible for "Shigellosis" or bacillary dysentery, an important cause of worldwide morbidity and mortality. However, despite this, there are very few epidemiological studies about this bacterium in Brazil. We studied the antibiotic resistance profiles and the clonal structure of 60 Shigella strains (30 S. flexneri and 30 S. sonnei) isolated from shigellosis cases in different cities within the metropolitan area of Campinas, State of São Paulo, Brazil. We used the following well-characterized molecular techniques: enterobacterial repetitive intergenic consensus, repetitive extragenic palindromic, and double-repetitive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shigella flexneri; Shigella sonnei; Antimicrobial resistance; Enterobacterial repetitive intergenic consensus- polymerase chain reaction; Repetitive extragenic palindromic-polymerase chain reaction; Double-repetitive element-polymerase chain reaction. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200012 |
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| Angelini,Michelle; Stehling,Eliana Guedes; Moretti,Maria Luiza; Silveira,Wanderley Dias da. |
| Shigella spp., the human pathogen responsible for shigellosis, is highly infectious even at low levels. The incidence rate of shigellosis varies with geographical distribution, location human development index, and age groups, being higher among children aged under 5 years. In Brazil, a few works indicate that shigellosis cases are underestimated, with S. flexneri and S. sonnei strains being the major agents responsible for the shigellosis cases. The present study used pulsed field gel electrophoresis (PFGE) to investigate the molecular epidemiology of 119 strains of S. sonnei and S. flexneri isolated from shigellosis cases that occurred in the metropolitan areas of Ribeirão Preto and Campinas Cities, São Paulo Sate, southeast Brazil. The results indicated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shigella sonnei; Shigella flexneri; Epidemiology; Characterization; PFGE. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300034 |
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| Elena,Grimaldi; Giovanna,Donnarumma; Brunella,Perfetto; Anna,De Filippis; Alessandro,Melito; Antonietta,Tufano Maria. |
| The recognition of bacterial components on the intestinal epithelial cells occurs through the toll-like receptors and is followed by the induction of an effective innate immune response. We analyzed receptor expression and signaling pathways involved in activation of human colon adenocarcinoma cells after stimulation with porins and LPS of Shigella flexneri. We also analyzed the expression and production of some cytokines, of intercellular adhesion molecule-1, of antimicrobial peptides human ²-defensins, and of the inducible form of nitric oxide synthase. Our data demonstrate that TLR2 is involved in porin recognition, whereas TLR4 with MD2, is required for LPS recognition. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Porins; TLRs; PAMPs; Shigella flexneri; Shigella flexneri. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300036 |
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