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食品における赤痢菌検出法の感度の向上 OAK
門田, 修子; 楠本, 晃子; 牧野, 壮一; 川本, 恵子; MONDEN, Shuko; KUSUMOTO, Akiko; MAKINO, Sou-ichi; KAWAMOTO, Keiko.
細菌性赤痢は,近年食品媒介感染が危惧されており,赤痢菌の食品からの迅速かつ高感度な検出法確立は急務であ る.本研究では,厚生労働省より配布された参考試験法中,検出結果に影響を与えると思われた手順に改良を加え新規 試験法とし,検出系の感度や迅速性の向上を図った.結果,新規試験法は食品検体からの赤痢菌検出において,参考試験法よりも10倍高感度であった.また,菌検出までに要する時間がおよそ21時間以内と,参考試験法より約3.5時間 の短縮ができた.以上より,新規試験法は迅速微量検出法であり,食品からの赤痢菌検出に有用であると考えられる. Outbreaks of shigellosis have been associated with traveling to areas with poor hygiene. However,the number of foodborne cases is increasing in industrialized countries. Since the infectious dose of Shigella spp. is as low as 10 cells,a rapid and sensitive method of detecting Shigella spp. in food is required. The Ministry of Health,Labour and Welfare of ]apan (MHLW) released a detection protocol for Shigella spp. from oysters in 2002,but a number of problems with it, including low...
Palavras-chave: ジルコニアビーズ破砕法; リアルタイムPCR; Shigella sonnei; Real-time PCR; Shigella sonnei; Zirconia beads breakage procedure.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2941
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Epidemiological characterization of resistance and PCR typing of Shigella flexneri and Shigella sonnei strains isolated from bacillary dysentery cases in Southeast Brazil BJMBR
Penatti,M.P.A.; Hollanda,L.M.; Nakazato,G.; Campos,T.A.; Lancellotti,M.; Angellini,M.; Brocchi,M.; Rocha,M.M.M.; Silveira,W. Dias da.
Shigella spp are Gram-negative, anaerobic facultative, non-motile, and non-sporulated bacilli of the Enterobacteriaceae family responsible for "Shigellosis" or bacillary dysentery, an important cause of worldwide morbidity and mortality. However, despite this, there are very few epidemiological studies about this bacterium in Brazil. We studied the antibiotic resistance profiles and the clonal structure of 60 Shigella strains (30 S. flexneri and 30 S. sonnei) isolated from shigellosis cases in different cities within the metropolitan area of Campinas, State of São Paulo, Brazil. We used the following well-characterized molecular techniques: enterobacterial repetitive intergenic consensus, repetitive extragenic palindromic, and double-repetitive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shigella flexneri; Shigella sonnei; Antimicrobial resistance; Enterobacterial repetitive intergenic consensus- polymerase chain reaction; Repetitive extragenic palindromic-polymerase chain reaction; Double-repetitive element-polymerase chain reaction.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200012
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Colicin type 7 produced by majority of Shigella sonnei isolated from Thai patients with diarrhoea BJM
Kaewklom,Siriporn; Samosornsuk,Seksun; Pipatsatitpong,Duangnate; Aunpad,Ratchaneewan.
Thirty one out of 153 strains of Shigella sonnei isolated from Thai patients with diarrhoea showed antibacterial activity against S. sonnei by agar well diffusion method. All of them harbor plasmids with the genetic determination of colicin type 7 (Js) gene but without colicin E and colicin U gene. The PCR product obtained from strain 35/44 was shown to be the gene for colicin type 7 lytic protein (cja). The partially purified bacteriocin (PPB) containing colicin type 7 of strain 35/44 was prepared and used for characterization. The antibacterial activity of PPB against a total of 17 selected Gram-positive and Gram-negative bacteria was tested. It was found that PPB of strain 35/44 was active against E. coli O157, S. sonnei and S. boydii. The sensitivity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shigella sonnei; Colicin type 7; Diarrhoea.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300010
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Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil BJM
Angelini,Michelle; Stehling,Eliana Guedes; Moretti,Maria Luiza; Silveira,Wanderley Dias da.
Shigella spp., the human pathogen responsible for shigellosis, is highly infectious even at low levels. The incidence rate of shigellosis varies with geographical distribution, location human development index, and age groups, being higher among children aged under 5 years. In Brazil, a few works indicate that shigellosis cases are underestimated, with S. flexneri and S. sonnei strains being the major agents responsible for the shigellosis cases. The present study used pulsed field gel electrophoresis (PFGE) to investigate the molecular epidemiology of 119 strains of S. sonnei and S. flexneri isolated from shigellosis cases that occurred in the metropolitan areas of Ribeirão Preto and Campinas Cities, São Paulo Sate, southeast Brazil. The results indicated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shigella sonnei; Shigella flexneri; Epidemiology; Characterization; PFGE.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300034
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Drug resistance profiles and clonality of sporadic Shigella sonnei isolates in Ankara, Turkey BJM
Kacmaz,Birgul; Unaldi,Ozlem; Sultan,Nedim; Durmaz,Riza.
The aims of this study were to investigate drug resistance rates, types of extended spectrum beta lactamases (ESBLs), and molecular epidemiological characteristics of 43 Shigella sonnei isolates. Ampicillin-sulbactam, amoxicillin-clavulanate, chloramphenicol, and ciprofloxacin were the most active antibiotics. Five isolates harbored blaSHV-12, blaTEM-1 and blaCTX-M-15. More than 90% of the isolates had an indistinguishable pulsotype.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shigella sonnei; Extended spectrum beta-lactamase; Plasmid profile; Pulsed-field gel electrophoresis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300013
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