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In vitro culture of Phyllanthus stipulatus (Euphorbiaceae) Rev. Bras. Bot.
CATAPAN,ELIZABETE; OTUKI,MICHEL FLEITH; VIANA,ANA MARIA.
An efficient micropropagation protocol was developed for the medicinal plant Phyllanthus stipulatus (Euphorbiaceae) using nodal segments for axillary shoot proliferation. Maximum multiplication rates (8-9 shoots per explant) was achieved on MS media supplemented with either 2.5-5.0 muM IBA. The best basal media for axillary shoot proliferation when 0.62 muM BA was supplemented were MS, MS/2 and AR (4-5 shoots per explant). Rooting was achieved with 100% of the microshoots on MS medium without growth regulators. Regenerated plants were successfully acclimatized and about 88% of plantlets survived under ex vitro conditions. Flowering was observed in 81% of the ex vitro grown plantlets after 12 weeks of acclimatization. High frequency callus initiation and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shoot culture; Acclimatization; Ex-vitro flowering; Callus culture; Root culture.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000100003
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Micropropagation of Salvia guaranitica Benth. through axillary shoot proliferation BABT
Echeverrigaray,Sergio; Carrer,Rosane Postingher; Andrade,Luciana Bavaresco.
Nodal segments from greenhouse grown adult plants of Salvia guaranitica were used to evaluate the effect of culture media and growth regulators on the micropropagation and growth. The highest multiplication rate was obtained on Murashige and Skoog (MS) medium supplemented with 2.22 µM of 6-benzylaminopurine (BAP). The best condition for rooting was MS medium with 2.85 µM indole-3-acetic acid (IAA). Rooted plants were successfully acclimatized and exhibited a normal development until maturity. Using the described protocol, approximately 35 plants per explant were obtained after three months.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Growth regulators; Shoot culture; Axillary buds.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400018
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