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Expression of the sigma35 and cry2AB genes involved in Bacillus thuringiensis virulence Scientia Agricola
Guidelli-Thuler,Ana Maria; Abreu,Irlan Leite de; Lemos,Manoel Victor Franco.
There are several genes involved in Bacillus thuringiensis sporulation. The regulation and expression of these genes results in an upregulation in Cry protein production, and this is responsible for the death of insect larvae infected by Bacillus thuringiensis. Gene expression was monitored in Bacillus thuringiensis during three developmental phases. DNA macroarrays were constructed for selected genes whose sequences are available in the GenBank database. These genes were hybridized to cDNA sequences from B. thuringiensis var. kurstaki HD-1. cDNA probes were synthesized by reverse transcription from B. thuringiensis RNA templates extracted during the exponential (log) growth, stationary and sporulation phases, and labeled with 33PadCTP. Two genes were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cry genes; Gene expression; Sigma factor.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300016
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