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	Provedor de dados BABT (2) Autor Benjamin,Sailas (2) Pandey,Ashok (2) Palavra-chave Candida rugosa (2) Characterization (2) Lipases (2) Purification (2) Solid cultures (2) Tipo do documento Info:eu-repo/semantics/article (2) Ano 2001 (1) 2000 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Isolation and characterization of three distinct forms of lipases from Candida rugosa produced in solid state fermentation BABT Benjamin,Sailas; Pandey,Ashok. Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40°C and pH 7-8.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida rugosa; Lipases; Solid cultures; Purification; Characterization. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200016 Isolation and characterization of three distinct forms of lipases from Candida rugosa produced in solid state fermentation BABT Benjamin,Sailas; Pandey,Ashok. Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40ºC and pH 7-8.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida rugosa; Lipases; Solid cultures; Purification; Characterization. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500002 Registros recuperados: 2 Primeira ... 1 ... Última

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