Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BJM (2) Autor Barcellos,Fernando Gomes (2) Pizzirani-Kleiner,Aline Aparecida (2) Hungria,Mariangela (1) Palavra-chave Somatic recombination (2) Heterokaryon (1) Limited vegetative compatibility (1) Parameiosis (1) Parasexuality (1) Trichoderma pseudokoningii (1) Unstable recombinants (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2011 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Genetic characterization of somatic recombination in Trichoderma pseudokoningii BJM Barcellos,Fernando Gomes; Pizzirani-Kleiner,Aline Aparecida. Crossing experiments via hyphal anastomosis between two strains contrasting for auxotrophic markers of Trichoderma pseudokoningii were conducted to characterize the somatic recombination process in this specie. Four crossings were made and a total of 1052 colonies obtained from conidial suspensions of the heterokaryotic colonies were analyzed. Sixty-eight recombinant colonies, from four growing generations, were analyzed for the auxotrophic markers. Of the 68 colonies analyzed, 58 were stable after four generations and the remainders were unstable, reverting to one of the parentals. Most of the recombinant colonies were unstable through subculture and after four growing generations they showed the leu ino met markers (auxotrophic for leucin, inositol and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Somatic recombination; Heterokaryon; Unstable recombinants; Parameiosis. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200012 Limited vegetative compatibility as a cause of somatic recombination in Trichoderma pseudokoningii BJM Barcellos,Fernando Gomes; Hungria,Mariangela; Pizzirani-Kleiner,Aline Aparecida. With the aim of a better characterization of the somatic recombination process in Trichoderma pseudokoningii, a progeny from crossings between T. pseudokoningii strains contrasting for auxotroph markers was characterized by RAPD markers and PFGE (electrophoretic karyotype). Cytological studies of the conidia, conidiogenesis and heterokaryotic colonies were also performed. The genotypes of the majority of the recombinant strains analyzed were similar to only one of the parental strains and the low frequency of polymorphic RAPD bands suggested that the nuclear fusions may not occur into the heterokaryon. In some heterokaryotic regions the existence of intensely staining hyphae might be related to cell death. We proposed that a mechanism of somatic... Tipo: Info:eu-repo/semantics/article Palavras-chave: Somatic recombination; Limited vegetative compatibility; Trichoderma pseudokoningii; Parasexuality. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400050 Registros recuperados: 2 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco