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Freitas,Priscila Ribeiro Corradi; Coelho,Sandra Gesteira; Rabelo,Euler; Lana,Ângela Maria Quintão; Artunduaga,Maria Alexandra Torres; Saturnino,Helton Mattana. |
Forty Holstein cows, with previous reproductive problems, empty, at second or more lactations, with an average milk production of 9,200 kg in the previous lactation and a dry period over than 50 days were distruibuted in two groups to evaluate two protocols of milking induction. It was given to group 1, on days one, eight and 21 recombinant bovine somatotropin-bSTr (500 mg); from day two to eight, estradiol cypionate (0.075 mg/kg BW) and medroxi progesterone acetate (0.25 mg/kg BW); from days nine to 15, estradiol cypionate (0.037 mg/kg BW); on day 19, prostaglandin F2α (0.530 mg) and from days 19 to 21, isoflupredone acetate (0.05 mg/kg BW). Group two was given, on days one, eight and 21, bSTr (500 mg), from days 2 to 15, estradiol benzoate (0.071 mg/kg... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Estradiol benzoate; Estradiol cypionate; Milk production; Somatotropin. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001000024 |
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Lew,Betina Joyce; Oliveira,Mauro Dal Secco de; Freitas Júnior,José Esler de; Carvalho,Marina Vieira de; Rêgo,Aníbal Coutinho do; Rennó,Francisco Palma. |
The objective of this study was to determine the effects of dietary energy and recombinant bovine somatotropin (bST) injection to identify genes that might control mammogenesis. Total RNA was extracted from the parenchymal tissue of 32 heifers randomly assigned to one of four treatments: two diets (a standard diet and a high energy, high protein diet), each with or without bST. To perform microarray experiments, RNA samples were pooled (2 animals/pool) before reverse transcription and labeling with Cy3 or Cy5. A 4-node loop design was used to examine the differential gene expression among treatments using a bovine-specific cDNA microarray (National Bovine Functional Genomics Consortium Library, NBFGC) containing 18,263 unique expressed sequence tags (EST).... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene regulation; Mammary gland development; Microarray; Nutrition; Somatotropin. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000700008 |
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