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| Registros recuperados: 24 | |
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| Oliveira,Andrey Carlos do Sacramento de; Pedroso,Silvia Cristina da Silva; Cardilli,Diogo José; Leite,Fábio Pereira Leivas; Ferreira,Gabrielle Virgínia Lopes; Silva,Andréia Silva da; Roos,Talita Bandeira; Moraes,Carina Martins de; Sousa,Roberta Sales; Monteiro,Roseli do Socorro Dias. |
| ABSTRACT: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Species identification; Bos taurus; Bubalusbubalis; DNA; Adulteration. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200652 |
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| Pinhal,Danillo; Gadig,Otto BF; Wasko,Adriane P; Oliveira,Claudio; Ron,Ernesto; Foresti,Fausto; Martins,Cesar. |
| Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chondrichthyes; PCR; Species identification; 5S rDNA; Sharks. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200033 |
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| CAMPOS, Z.; SANAIOTTI, T.; MAGNUSSON, W.. |
| O objetivo deste estudo é divulgar os avanços de conhecimento da biologia da reprodução de jacaré-paguá, Paleosuchus palpebrosus, nos diferentes ambientes da floresta alagada da Amazônia e nos riachos do entorno do Pantanal. Os resultados apresentados encontram-se alinhados ao Objetivo de Desenvolvimento Sustentável das Nações Unidas n° 15 - Vida Terrestre, no que diz respeito à sua Meta 15.1.- assegurar a conservação, recuperação e uso sustentável de ecossistemas terrestres e de água doce interiores e seus serviços, em especial florestas, zonas úmidas, montanhas e terras áridas, em conformidade com as obrigações decorrentes dos acordos internacionais (Nações Unidas Brasil, 2022). |
| Tipo: Folhetos |
Palavras-chave: Selo ODS 15; Jacaré; Preservação da Natureza; Animal Selvagem; Caiman; Habitat preferences; Species identification. |
| Ano: 2022 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1148401 |
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| Griffiths, Andrew M.; Sotelo, Carmen G.; Mendes, Rogerio; Perez-martin, Ricardo I.; Schroeder, Ute; Shorten, Marc; Silva, Helena A.; Verrez-bagnis, Veronique; Mariani, Stefano. |
| Mislabelling of food products has recently received a great deal of public scrutiny, but it remains unclear exactly what methods are being utilised in laboratories testing the authenticity of foods. In order to gain insight into the specific area of the analysis of seafood, a questionnaire focusing on the taxonomic groups typically analysed and the techniques utilised was sent to over one hundred accredited laboratories across the UK, Ireland, Spain, Portugal, France and Germany. Forty-five responded positively, demonstrating significant differences in both the species analysed and methods utilised among the countries included in the survey. Indeed, a diversity of methods was employed across laboratories and efforts to harmonise and/or standardise testing... |
| Tipo: Text |
Palavras-chave: Food testing; Forensically informative nucleotide sequencing; Species identification; DNA barcoding; Fisheries. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00188/29918/28383.pdf |
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| Bojolly, Daline; Doyen, Perine; Le Fur, Bruno; Christaki, Urania; Verrez-bagnis, Veronique; Grard, Thierry. |
| Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, as juveniles of bigeye and yellowfin tunas are very difficult to distinguish, unintentional substitutions may occur during the canning process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 and cytochrome c oxidase subunit II genes were used to identify bigeye tuna and yellowfin tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one... |
| Tipo: Text |
Palavras-chave: Tuna; Authentication; Quantification; Canned products; TaqMan; QPCR; Species identification; Bigeye tuna (Thunnus obesus); Yellowfin tuna (Thunnus albacares). |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00371/48217/48403.pdf |
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| Besbes, Nadia; Jerome, Marc; Berge, Jean-pascal; Sadok, Saloua. |
| This study describes a polymerase chain reaction using restriction fragment length polymorphism (PCR-RFLP) assay based on the 16S rRNA mitochondrial gene to identify commercial food products of wide range of Penaeidae and Pandalidae shrimp species commercialised in the Tunisian market. Phylogenetic analyses on 16S rRNA mitochondrial gene were used to study the relationships among the considered species. Penaeidae shrimp species was easily differentiated and confirmed by direct sequencing, showing a genetic distance of 0.34 with respect to Pandalidae species. A rapid and reliable PCR method using restriction fragment length polymorphism (RFLP) with three restriction enzymes (HpyCH4III/ MboI / AluI) was optimized for unambiguous differentiation of shrimp... |
| Tipo: Text |
Palavras-chave: Shrimps; Species identification; 16S rRNA mtDNA; PCR-RFLP. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00303/41445/40637.pdf |
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| Biscoito, Manuel; Segonzac, Michel; Almeida, Armando; Desbruyeres, Daniel; Geistdoerfer, Patrick; Turnipseed, Mary; Van Dover, Cindy. |
| Introduction : Since the discovery of animal communities in oceanic hydrothermal vents in 1977 and in deep-sea cold seeps in 1984 (Londsdale, 1977; Paull et al., 1984) fishes have been regularly observed in association with these chemosynthetically-driven communities, but in most cases they are difficult to catch and therefore species identification can only rely on images taken by the diving vehicles. Ichthyological information pertaining to species inhabiting the deep-sea hydrothermal vents and cold seeps is mentioned in over 30 papers and even in the more detailed and updated lists (Geistdoerfer, 1991; 1996; 1999; Sibuet & Olu, 1998; Tunnicliffe, 1991) there are species missing. The situation is even less clear concerning the bathyal species... |
| Tipo: Text |
Palavras-chave: Fishes; Species identification; Hydrothermal vent. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-896.pdf |
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| Berger, Laurent; Poncelet, Cyrille; Trenkel, Verena. |
| Fish schools can be insonified simultaneously with multifrequency echosounders (e.g. Simrad EK60s) and a multibeam echosounder (e.g. Simrad ME70). This paper presents a method for combining these data to improve estimates of the relative frequency response r(f) of fish schools. Values of r(f) are now commonly used to classify echoes in fishery surveys. The data from the roll- and pitch-stabilized, high-resolution ME70 are used to correct beam-width effects in the multifrequency EK60 data. First, knowing the exact position and orientation of the transducers and the position of the vessel, the echoes are placed into a common geographic coordinate system. Then, the EK60 data are rejected if they do not include a significant percentage of the fish school... |
| Tipo: Text |
Palavras-chave: Species identification; Scomber scombrus; Sardina pilchardus; Pelagic fish; Acoustics. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6590.pdf |
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| Yasuda, Nina; Taquet, Coralie; Nagai, Satoshi; Fortes, Miguel; Fan, Tung-yung; Harii, Saki; Yoshida, Terutoyo; Sito, Yuta; Nadaoka, Kazuo. |
| Examining genetic diversity and lineage sorting of different genes in closely related species provide useful information for phylogenetic analyses and ultimately for understanding the origins of biodiversity. In this study, we examined inter- and intraspecific genetic variation in internal transcribed spacer 2 (ITS2), partial mitochondrial gene (mtMutS), and nuclear microsatellite flanking region in two closely related octocoral species (Heliopora coerulea, HC-A and HC-B). These species were recently identified in a population genetic study using microsatellite markers. The two species have different reproductive timing, which ecologically promotes lineage sorting. In this study, we examined whether species boundaries could be detected by the commonly used... |
| Tipo: Text |
Palavras-chave: ITS2; Concerted evolution; MtMutS; Secondary structure; Species identification; Speciation. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00667/77932/80144.pdf |
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| Thomas, Yoann; Belliard, Corinne; Garen, Pierre; Gueguen, Yannick; Montagnani, Caroline. |
| In French Polynesia, the black-lip pearl oyster Pinctada margaritifera has been farmed to produce pearls since the 1980s, forming the basis of a major industry. The sustainability of this activity relies on spat collection in the lagoons. However, pearl oyster spat can be difficult to identify for the evaluation of stock variations. It is especially hard to distinguish Pinctada spp. larvae at a very early stage of development. In the present study, a whole- mount in situ hybridisation (ISH) technique was developed to allow the discrimination of larvae of closely-related pearl oyster species found in the French Polynesian atolls. Using specific ribosomal 16S-DNA sequence data, we were able to successfully differentiate between Pinctada margaritifera and... |
| Tipo: Text |
Palavras-chave: Whole larvae in situ hybridisation; 16S rRNA; Plankton; Species identification; Pearl oyster; Pinctada margaritifera. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00035/14603/11923.pdf |
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| Korneliussen, R; Diner, Noel; Ona, E; Berger, Laurent; Fernandes, P. |
| Acoustic surveys are used to estimate the abundance and distribution of many fish species, and have been based traditionally on data collected at a single acoustic frequency. Although it has been known for some time that the use of additional frequencies can provide information on the nature of the acoustic target, the knowledge and technology required to combine the so-called "multifrequency data" in an appropriate manner has been limited. The use of several transducers of different frequencies is now common on board research vessels and fishing vessels, so multifrequency data are often collected. In order for these data to be combined appropriately, their physical and spatial characteristics from each frequency should be as similar as possible. We detail... |
| Tipo: Text |
Palavras-chave: Species identification; Multifrequency acoustics; Data collection. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-5286.pdf |
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| Registros recuperados: 24 | |
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