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	Provedor de dados BJMBR (2) International Journal of Morphology (1) Autor Abaidoo,C.S (1) Andrews,P. W (1) Boateng,K. A (1) Chimelli,L. (1) Hongfang,W. (1) Jianmin,Z. (1) Martinez,A.M.B. (1) Meifu,F. (1) Mendonça,D.M.F. (1) Warren,M. A (1) Mais... Palavra-chave Spheroids (3) Amyotrophic lateral sclerosis (1) BeWo (1) Drug resistance (1) Epirubicin (1) Hepatocellular carcinoma (1) Immunohistochemistry (1) Microcarrier (1) Morphometry (1) Neurofilament proteins (1) Trophoblast (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Journal article (1) Ano 2010 (1) 2005 (1) 2002 (1) País Brazil (2) Chile (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Resistance of multicellular aggregates to pharmorubicin observed in human hepatocarcinoma cells BJMBR Jianmin,Z.; Hongfang,W.; Meifu,F.. The objective of the present study was to investigate the multicellular resistance of human hepatocarcinoma cells BEL-7402 to pharmorubicin. Cells (1 x 10(4)) and 200 microcarrier Cytodex-3 beads were seeded onto a 24-well plate and cultured in RPMI 1640 medium. After the formation of multicellular aggregates, morphology and cell viability were analyzed by scanning electron microscopy, transmission electron microscopy and flow cytometry, respectively. The IC50 was determined by flow cytometry and MTT assay after the cells cultured in aggregates and monolayers were treated with pharmorubicin. The culture products exhibited structural characteristics somewhat similar to those of trabecular hepatocarcinoma in vivo. Among the microcarriers, cells were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hepatocellular carcinoma; Spheroids; Drug resistance; Microcarrier; Epirubicin. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200015 Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis BJMBR Mendonça,D.M.F.; Chimelli,L.; Martinez,A.M.B.. Amyotrophic lateral sclerosis (ALS), a neurodegenerative disease of unknown etiology, affects motor neurons leading to atrophy of skeletal muscles, paralysis and death. There is evidence for the accumulation of neurofilaments (NF) in motor neurons of the spinal cord in ALS cases. NF are major structural elements of the neuronal cytoskeleton. They play an important role in cell architecture and differentiation and in the determination and maintenance of fiber caliber. They are composed of three different polypeptides: light (NF-L), medium (NF-M) and heavy (NF-H) subunits. In the present study, we performed a morphological and quantitative immunohistochemical analysis to evaluate the accumulation of NF and the presence of each subunit in control and ALS... Tipo: Info:eu-repo/semantics/article Palavras-chave: Amyotrophic lateral sclerosis; Spheroids; Neurofilament proteins; Immunohistochemistry. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600015 A Quantitative Assessment of the Morphological Characteristics of BeWo Cells as an in vitro Model of Human Trophoblast Cells International Journal of Morphology Abaidoo,C.S; Warren,M. A; Andrews,P. W; Boateng,K. A. In order to study the detailed morphology of trophoblast cells during human implantation, BeWo cells were cultured as spheroids in suspension culture. These cultures were then processed for light and electron microscopical examination. The present study showed that the BeWo spheroids consist of two cell types which are cytotrophoblast-like and syncytiotrophoblast-like. The cells with larger nuclear diameter made up only about 1% of the cell population and appear to be those of syncytiotrophoblast. Therefore the predominant cell type of the BeWo spheroids appeared to be relatively undifferentiated and cytotrophoblast-like. About 10% of the BeWo cells in the present study were mitotic, indicating a highly proliferative population. Total cell number increased... Tipo: Journal article Palavras-chave: BeWo; Trophoblast; Morphometry; Spheroids. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000400011 Registros recuperados: 3 Primeira ... 1 ... Última

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