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An analysis of the spontaneous mutation rate measurement in filamentous fungi Genet. Mol. Biol.
Baracho,Marta S.; Baracho,Ivanhoé R..
Mutations related to gene methG1 of Aspergillus nidulans were analyzed, in order to study a mathematical model for the determination of the mutation rate per nucleus per generation, in filamentous fungi. A replica plating technique was used to inoculate, in a single operation, 26 colonies of the strain, into Petri dishes containing culture medium, and the nine central colonies were analyzed for size and number of conidia in each colony. Using this technique, several central colonies were analyzed with regard to the appearance of mutation, and the number and type of reversions were determined for each colony. The frequencies obtained for each reversion were analyzed, in order to verify if their distribution was in accordance with that of Greenwood and Yule....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus nidulans; Mutation rate; Spontaneous mutation; Fungi; Filamentous fungi.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100014
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Study on the susceptibility of Enterococcus faecalis from infectious processes to ciprofloxacin and vancomycin J. Venom. Anim. Toxins incl. Trop. Dis.
Genaro,A.; Cunha,M. L. R. S.; Lopes,C. A. M..
Enterococcus faecalis is considered a pathogen responsible for hospital infections and, due to its frequent multi-resistant profile, has caused preoccupations among many medical authorities. The objective of this study was to determine the antimicrobial susceptibility of 74 strains isolated from blood cultures and purulent secretions to vancomycin and ciprofloxacin through the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) by using the Microdilution test. The results showed a greater efficacy of vancomycin compared to ciprofloxacin (98.6% of the strains were inhibited by vancomycin at lower concentrations: 0.06 - 1 µg/ml). However, in the MBC analysis 73% of the strains showed a MBC of vancomycin only at high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococcus faecalis; Vancomycin; Ciprofloxacin; Resistance; Spontaneous mutation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300004
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