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de Almeida,R.W.; Espírito-Santo,M.P.; Sousa,P.S.F.; de Almeida,A.J.; Lampe,E.; Lewis-Ximenez,L.L.. |
We evaluated the stability of hepatitis B virus (HBV) DNA in plasma samples stored at 42°C for external quality assessment (EQA) panels of viral load. To assess the stability of plasma samples containing different concentrations of HBV DNA, serial dilutions of HBV-infected samples with a viral load of 6.40 log(10) IU/mL were made to yield viral loads of 5, 4, and 3 log(10) IU/mL. These were incubated at 42°C for up to 7 days and then frozen at -70°C. Viral load testing for HBV DNA was performed for all samples using COBAS¯ AmpliPrep/COBAS¯ TaqMan¯ HBV Test (v.2.0, Roche, Switzerland). Results were compared with fresh frozen plasma samples as a benchmark to establish acceptable measurements on the days following sample collection. Although the results of... |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: HBV; Stability studies; Real-time PCR. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600553 |
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Rodrigues,Rayllan de Oliveira; Damasceno,Gabriel Azevedo de Brito; Barreto,Stella Maria Andrade Gomes; Fernandes,Julia Morais; Telaprolu,Krishna Chaitanya; Rocha-Filho,Pedro Alves da; Soares,Luiz Alberto Lira; Ostrosky,Elissa Arantes; Sales,Valeria Soraya de Farias; Langassner,Silvana Maria Zucolotto; Ferrari,Márcio. |
The purpose of this study was to evaluate the safety of Kalanchoe brasiliensis extract, followed by the development of an oil in water emulsion containing the K. brasiliensis leaves extract and evaluating its clinical moisturizing efficacy. The formulations containing sodium acrylates/ Beheneth-25 methacrylate Crosspolymer (and) hydrogenated polydecene (and) lauryl glucoside and 0.5% of extract were prepared. The extract was considered as non-irritating through skin irritant tests. The stability testing was carried out in different conditions for 90 days. The skin hydration was measured by capacitance measurement and transepidermal water loss using biophysical techniques. The results indicate that the formulation containing 0.5% of extract increased the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cosmetics/products; Kalanchoe brasiliensis/extract/safety evaluation; Kalanchoe brasiliensis/moisturizing efficacy; Stability studies; Brazil/plants. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400624 |
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