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	Provedor de dados BJMBR (1) Autor Alvares,L.E. (1) Corrente,J.E. (1) Coutinho,L.L. (1) Mantoani,A. (1) Palavra-chave Gene expression (1) Myogenic regulatory factors (1) Quantitative RT-PCR (1) Standard-curve (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2003 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Standard-curve competitive RT-PCR quantification of myogenic regulatory factors in chicken embryos BJMBR Alvares,L.E.; Mantoani,A.; Corrente,J.E.; Coutinho,L.L.. The reverse transcription-polymerase chain reaction (RT-PCR) is the most sensitive method used to evaluate gene expression. Although many advances have been made since quantitative RT-PCR was first described, few reports deal with the mathematical bases of this technique. The aim of the present study was to develop and standardize a competitive PCR method using standard-curves to quantify transcripts of the myogenic regulatory factors MyoD, Myf-5, Myogenin and MRF4 in chicken embryos. Competitor cDNA molecules were constructed for each gene under study using deletion primers, which were designed to maintain the anchorage sites for the primers used to amplify target cDNAs. Standard-curves were prepared by co-amplification of different amounts of target cDNA... Tipo: Info:eu-repo/semantics/article Palavras-chave: Quantitative RT-PCR; Standard-curve; Myogenic regulatory factors; Gene expression. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200004 Registros recuperados: 1 Primeira ... 1 ... Última

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