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	Provedor de dados BJMBR (2) Autor Langella,P. (2) Adel-Patient,K. (1) Azevedo,V. (1) Bermúdez-Humarán,L.G. (1) Chatel,J.M. (1) Commissaire,J. (1) Gruss,A. (1) Le Loir,Y. (1) Nouaille,S. (1) Wal,J.M. (1) Mais... Palavra-chave Lactococcus lactis (2) Protein secretion (2) Staphylococcal nuclease (2) Allergen (1) Bovine coronavirus (1) Epitope (1) LEISSTCDA (1) Propeptide (1) SS-lactoglobulin (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2005 (1) 1999 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Improvement of bovine ß-lactoglobulin production and secretion by Lactococcus lactis BJMBR Nouaille,S.; Bermúdez-Humarán,L.G.; Adel-Patient,K.; Commissaire,J.; Gruss,A.; Wal,J.M.; Azevedo,V.; Langella,P.; Chatel,J.M.. The stabilizing effects of staphylococcal nuclease (Nuc) and of a synthetic propeptide (LEISSTCDA, hereafter called LEISS) on the production of a model food allergen, bovine ß-lactoglobulin (BLG), in Lactococcus lactis were investigated. The fusion of Nuc to BLG (Nuc-BLG) results in higher production and secretion of the hybrid protein. When LEISS was fused to BLG, the production of the resulting protein LEISS-BLG was only slightly improved compared to the one obtained with Nuc-BLG. However, the secretion of LEISS-BLG was dramatically enhanced (~10- and 4-fold higher than BLG and Nuc-BLG, respectively). Finally, the fusion of LEISS to Nuc-BLG resulting in the protein LEISS-Nuc-BLG led to the highest production of the hybrid protein, estimated at ~8 µg/ml... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactococcus lactis; Protein secretion; LEISSTCDA; Staphylococcal nuclease; SS-lactoglobulin; Allergen. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300005 Heterologous protein secretion in Lactococcus lactis: a novel antigen delivery system BJMBR Langella,P.; Le Loir,Y.. Lactic acid bacteria (LAB) are Gram-positive bacteria and are generally regarded as safe (GRAS) organisms. Therefore, LAB could be used for heterologous protein secretion and they are good potential candidates as antigen delivery vehicles. To develop such live vaccines, a better control of protein secretion is required. We developed an efficient secretion system in the model LAB, Lactococcus lactis. Staphylococcal nuclease (Nuc) was used as the reporter protein. We first observed that the quantity of secreted Nuc correlated with the copy number of the cloning vector. The nuc gene was cloned on a high-copy number cloning vector and no perturbation of the metabolism of the secreting strain was observed. Replacement of nuc native promoter by a strong... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protein secretion; Staphylococcal nuclease; Lactococcus lactis; Propeptide; Bovine coronavirus; Epitope. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200007 Registros recuperados: 2 Primeira ... 1 ... Última

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