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Correlation of phenotypic tests with the presence of the blaZ gene for detection of beta-lactamase BJM
Ferreira,Adriano Martison; Martins,Katheryne Benini; Silva,Vanessa Rocha da; Mondelli,Alessandro Lia; Cunha,Maria de Lourdes Ribeiro de Souza da.
Abstract Staphylococcus aureus and Staphylococcus saprophyticus are the most common and most important staphylococcal species associated with urinary tract infections. The objective of the present study was to compare and to evaluate the accuracy of four phenotypic methods for the detection of beta-lactamase production in Staphylococcus spp. Seventy-three strains produced a halo with a diameter ≤28 mm (penicillin resistant) and all of them were positive for the blaZ gene. Among the 28 susceptible strain (halo ≥29 mm), 23 carried the blaZ gene and five did not. The zone edge test was the most sensitive (90.3%), followed by MIC determination (85.5%), but the specificity of the former was low (40.0%). The nitrocefin test was the least sensitive (28.9%)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Urinary tract infection; Staphylococcus saprophyticus; Zone edge test; Beta-lactamase; BlaZ.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100159
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