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| Vasconcelos,R.B.; Salles,L.P.; Oliveira e Silva,I.; Gulart,L.V.M.; Souza,D.K.; Torres,F.A.G.; Bocca,A.L.; Rosa e Silva,A.A.M.. |
| Follicle cultures reproduce in vitro the functional features observed in vivo. In a search for an ideal model, we cultured bovine antral follicle wall sections (FWS) in a serum-free defined medium (DM) known to induce 17β-estradiol (E2) production, and in a nondefined medium (NDM) containing serum. Follicles were sectioned and cultured in NDM or DM for 24 or 48 h. Morphological features were determined by light microscopy. Gene expression of steroidogenic enzymes and follicle-stimulating hormone (FSH) receptor were determined by RT-PCR; progesterone (P4) and E2 concentrations in the media were measured by radioimmunoassay. DM, but not NDM, maintained an FWS morphology in vitro that was similar to fresh tissue. DM also induced an increase in the expression... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovary follicle wall section; FSH receptor; Gene expression; Steroidogenic enzymes; Hormone steroids; Morphological aspect. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800700 |
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