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Pollen analysis of the post-emergence residue of Melipona (Melikerria) interrupta Latreille (Hymenoptera: Apidae) bred in the central Amazon region Acta Botanica
Ferreira,Marcos Gonçalves; Absy,Maria Lúcia.
We applied an "adapted" protocol for collecting and processing pollen grains in the pollen analysis of the post-emergence residue of Melipona (Melikerria) interrupta Latreille. The study was conducted at the Sant'Ana honey farm, located on the banks of the Solimões River, in the municipality of Manacapuru, in the state of Amazonas, Brazil, where a colony was monitored in October and November 2010. From that colony, 10 samples of post-emergence residue were collected. Unlike in the acetolysis method, there was no need to expose pollen grains to an acidic medium, because pollen loses its content during the larval digestive process. We identified 32 pollen types, from 19 botanical families, plus three undetermined pollen types. The most representative family...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Palynology; Pollen; Floral resources.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400009
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Stingless bees and mass flowering trees in the canopy of Atlantic Forest: a tight relationship Acta Botanica
Ramalho,Mauro.
This study demonstrates that stingless bees (Apidae, Hymenoptera) amount to approximately 70% of all bees foraging on flowers in the Brazilian Tropical Atlantic Rainforest. They also are the unique bee group concentrated in the upper stratum. It is hypothesized that this vertical distribution is a result of an uneven distribution of preferred floral resources within the forest strata. In the upper stratum, most of the trees that are highly visited by stingless bees produce small, inconspicuous, generalized flowers, clustered in dense inflorescences (mass flowering). These trees represent only 15% of the total melittophilous flora in the study area (96 plant species). In contrast, they attracted all 17 stingless bee species and more than 70% of all...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Forest canopy; Foraging stratification; Mass flowering; Atlantic forest.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062004000100005
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Pollen sources used by Frieseomelitta Ihering 1912 (Hymenoptera: Apidae: Meliponini) bees along the course of the Rio Negro, Amazonas, Brazil Acta Botanica
Pimentel,Alyne Daniele Alves; Absy,Maria Lucia; Rech,André Rodrigo; Abreu,Vanessa Holanda Righetti de.
ABSTRACT Insect pollination has influenced the evolution and diversification of angiosperms. Consequently, knowing plants used as food sources by bees, the most important pollinator group, is the first step toward understanding how their ecological relationships works. Pollen source information is also highly relevant for bee management and associated fruit and seed production. Accordingly, to improve understanding of the trophic ecology of these bees and their relationships with native Amazonian plants the current study identified, quantified and compared pollen stored in nests of Frieseomelitta stingless bees along the Rio Negro, Amazonas, Brazil. A total of 31 pollen pots were sampled and found to contain 65 pollen types distributed across 52 genera and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazon; Feeding behavor; Meliponicultura; Stingless bees; Trophic resources.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000200371
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Areas of natural occurrence of Melipona scutellaris Latreille, 1811 (Hymenoptera: Apidae) in the state of Bahia, Brazil Anais da ABC (AABC)
Alves,Rogério M.O.; Carvalho,Carlos A.L.; Souza,Bruno A.; Santos,Wyratan S..
The bee Melipona scutellaris is considered the reared meliponine species with the largest distribution in the North and Northeast regions of Brazil, with records from the state of Rio Grande do Norte down to the state of Bahia. Considering the importance of this species in the generation of income for family agriculture and in the preservation of areas with natural vegetation, this study aimed at providing knowledge on the distribution of natural colonies of M. scutellaris in the state of Bahia. Literature information, interviews with stinglessbee beekeepers, and expeditions were conducted to confirm the natural occurrence of the species. A total of 102 municipalities showed records for M. scutellaris, whose occurrence was observed in areas ranging from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bee beekeeping; Uruçu bee; Stingless bees; Distribution.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300010
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Labor division in Melipona compressipes fasciculata Smith (Hymenoptera: Apidae: Meliponinae) Anais da SEB
Giannini,Katia M..
Labor division in the stingless bee Melipona compressipes fasciculata Smith was analyzed and compared with that of other eusocial bees Meliponini and Trigonini. The results showed a sequence of activities which was similar to that of other stingless bees already studied, such as: grooming; cerumen work; cell construction; cleaning; the colony; provisioning and oviposition process; ventilation; enclosure gap; nectar reception; and foraging.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Meliponini; Stingless bees; Division of labor; Eusocial bees.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591997000100020
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Bionomics of Melipona mondury Smith 1863 (Hymenoptera: Apidae, Meliponini) in relation to its nesting behavior Biota Neotropica
Viana,Jaqueline Lemos; Sousa,Henrique de Abreu Cerqueira; Alves,Rogério Marcos de Oliveira; Pereira,Derval Gomes; Silva Jr.,Juvenal Cordeiro; Paixão,Joana Fidelis da; Waldschmidt,Ana Maria.
Melipona mondury Smith 1863 is an important stingless bee species pollinator and honey/pollen producer, but threatened by fragmentation of habitats throughout its range. This article presents the identification and partial characterization of nesting substrates, bionomic including population features of M. mondury by comparing data between nest architecture of natural beehive and boxes colonies. Nineteen colonies (13 in boxes and six in beehives) were analyzed with regards to substrate nesting, nest characteristics (e.g. length and width of the combs, height and diameter of the pollen and honey jar) and bee population. The average volume and diameter of the trunks cavities used for nesting of M.mondury were18.4 L and 15.8 cm; the average number of combs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation; Nest architecture; Nesting substrate; Rational management; Stingless bees.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032015000300102
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Diversity of Meliponini and anothers Apiformes (Apidae sensu lato) in a Cerrado fragment and its surrounding, Campo Grande, MS Biota Neotropica
Roel,Antonia Railda; Peruca,Ricardo Dias; Lima,Felipe Varussa de Oliveira; Cheung,Kwok Chiu; Araujo Neto,Armando; Silva,Leticia Vieira Da; Soares,Suellen.
Abstract: The aim of this study was to evaluate the diversity of Meliponini and anothers Apiformes (Apidae sensu lato) from a Cerrado fragment and its surrounding. Malaise traps were installed in a pasture near a fragment of Cerrado and its surrounding, from October to December 2010. Samples were collected at seven-day intervals and preserved in Dietrich solution. The apiforms specimens from each sample were separated in morphospecies and sent to identification. The tribe Meliponini was the most abundant in open areas of pasture than in the Cerrado fragment. The Meliponini species present were Trigona gr. fulviventris, Paratrigona lineata, Trigona recursa, Partamona sp, Trigona spinipes, Trigona gr. hyalinata, Trigona gr. fulviventris, Tetragonisca...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Native bees; Pollinators; Stingless bees.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032019000200210
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Plants and pollinating bees in Maringá, State of Paraná, Brazil BABT
Toledo,Vagner de Alencar Arnaut de; Fritzen,Adriano Ernesto de Toledo; Neves,Carolina Antunes; Ruvolo-Takasusuki,Maria Claudia Colla; Sofia,Silvia Helena; Terada,Yoko.
The present study was carried out to survey the bees as visitors to melliferous flora in the region of Maringá, state of Paraná, Brazil. A total of 331 insects were captured, and the fauna comprised 39.88% Trigona spinipes, 38.37% Apis mellifera, 8.16% Tetragonisca angustula, 3.93% Halictidae, 1.21% Megachilidae, 2.42% Anthophoridae, and 3.32% other Hymenoptera. Eleven plant species from nine families were observed. The four families most frequently visited by A. mellifera were Pontederiaceae (93.53%), Sterculiaceae and Polygoniaceae (47.22%), Apocynaceae and Apiaceae (42.86%). The families most visited by T. spinipes were Lamiaceae (64.70%), Apocynaceae (57.14%), Sterculiaceae (51.85%) and Anacardiaceae (48.39%), and the families most visited by T....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Pollination; Melliferous flora.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400025
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Responses to climatic factors by foragers of Plebeia pugnax Moure (in litt.) (Apidae, Meliponinae) BJB
HILÁRIO,S. D.; IMPERATRIZ-FONSECA,V. L.; KLEINERT,A. de M. P..
Flight activity of Plebeia pugnax Moure (in litt.) was studied in six colonies coming from Cunha, SP, from July to October 1994. Twice a week, from 8:00 a.m. to 6:00 p.m., for 5 minutes every half-hour, all the bees entering and leaving the hives were counted. Six hundred counts were made and the materials that foragers carried were recorded. Data were analysed in relation to temperature, relative humidity, light intensity and day time. Foragers' flight activity was relatively constant in a wide range of temperature, from 22°C to 34°C. The minimum temperature for the beginning of flight activity was 14°C. Effective flight activity (when foragers of all colonies were leaving the hives) occurred at 15°C. These bees also flew within a wide range of relative...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flight activity; Stingless bees; Climatic factors.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082001000200003
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Melipona garbage bees release their cargo according to a Gaussian distribution BJB
KERR,A. S.; KERR,W. E..
Worker bees of Melipona compressipes and Melipona scutellaris (Meliponinae, Apidae) colonies, involved in carrying garbage to the outside of the nest, dump each individual load cargo of cocoon pellets (cocoon + larval feces + remaining wax). For Melipona scutellaris they weight a mean of 27.8 mg, and are dropped at a distance of 1 to 45 m and between 1 and 35 m for M. compressipes, according to a Gaussian Distribution. In the case of M. scutellaris the expression is: <img src="http:/img/fbpe/rbbio/v59n1/a15e1.gif"> where, N(x) = number of pellets; Exp = e elevated to the expression in brackets; x = distance in meters; the linear term (bx) describes accidental cargo slipping. The constants for the observed data are: b = 0.69; A = 1363; sigma = 2.16 m;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Melipona scutellaris; Melipona compressipes; Garbage bees; Gaussian Distribution; Garbage release.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71081999000100015
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Flight activity and colony strength in the stingless bee Melipona bicolor bicolor (Apidae, Meliponinae) BJB
HILÁRIO,S. D.; IMPERATRIZ-FONSECA,V. L.; KLEINERT,A. de M. P..
Flight activity of Melipona bicolor bicolor, coming from Cunha (23º05'S, 44º55'W), Atlantic Forest, was studied in ten colonies, and in two periods: from July to September 1993 and from August to September 1995. The colonies were grouped in weak, medium and strong, according to the diameter of the combs, which can provide a good idea of the number of cells built. 855 observations were accomplished for 5 minutes, every half-hour, from 8 to 18 hours. The total number of bees that entered and left the hive and the number of bees that arrived with mud, pollen and resin, besides the number that went out with debris in that period were counted. It was also registered the temperature and the relative humidity of the air. The total external activity, as well as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Flight activity; Climate conditions; Resource collection.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000200014
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Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees BJMBR
Torres,A.R.; Sandjo,L.P.; Friedemann,M.T.; Tomazzoli,M.M.; Maraschin,M.; Mello,C.F.; Santos,A.R.S..
In this study, we investigated the chemical composition, and antioxidant and antibacterial properties of ethanolic extracts of propolis (EEP) from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula. Chemical composition of EEP was determined by colorimetry and chromatographic (HPLC-DAD and UPLC-Q/TOF-MS/MS) analysis. Antimicrobial activity of EEP was evaluated against gram-positive (S. aureus, methicillin-resistant S. aureus, E. faecalis) and gram-negative (E. coli and K. pneumoniae) bacteria by the minimal inhibitory concentration (MIC) test using the microdilution method. Furthermore, the growth curve and integrity of cell membrane of S. aureus and E. coli were investigated using standard microbiological methods. HPLC-DAD analysis showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stingless bees; Propolis; Antimicrobial activity; S. aureus; M. quadrifasciata quadrifasciata; T. angustula.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600617
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Pythium insidiosum: inhibitory effects of propolis and geopropolis on hyphal growth BJM
Araújo,Maria José Abigail Mendes; Bosco,Sandra de Moraes Gimenes; Sforcin,José Maurício.
Abstract Propolis and geopropolis are resinous products of bees showing antimicrobial effects. There is no data concerning their action against Pythium insidiosum - the causative agent of pythiosis, a pyogranulomatous disease of the subcutaneous tissue that affects mostly horses, dogs and humans. Fragments of 15 isolates of P. insidiodum were incubated with propolis and geopropolis extracts and evaluated for up to seven days to detect the minimal fungicidal concentration (MFC). Propolis inhibited three isolates at 1.0 mg mL-1 after 24 h and all other isolates at 3.4 mg mL-1. Geopropolis led to more variable results, exerting predominantly a fungistatic action than a fungicidal one. Propolis was more efficient than geopropolis in inhibiting P. insidiosum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pythium insidiosum; Propolis; Geopropolis; Stingless bees; Africanized honeybees.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400863
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Antibacterial activity of propolis produced by Frieseomelitta varia Ciência e Agrotecnologia
Campos,Viviane Aparecida Costa; Santos Júnior,Helvécio Martins dos; Oliveira,Denilson F; Carvalho,Hudson Walace Pereira de; Machado,Alan Rodrigues Teixeira; Tirelli,Aline Auxiliadora.
To contribute to the development of antibacterial products from propolis produced by native Brazilian bees, twenty-nine samples of propolis collected from hives in the state of Minas Gerais, Brazil, were screened for in vitro activity against Aeromonas hydrophila, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus. Among the samples from native Brazilian bees, only that from Frieseomelitta varia (Lepeletier, 1836) inhibited in vitro bacterial growth. Consequently, this propolis underwent fractionation by chromatographic methods monitored through Agar-diffusion assays with these bacteria, which resulted in the isolation and identification of 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C), which showed MIC of 62.5 and 250 µg/mL against...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artepillin C; Stingless bees; Antibiotic.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000600002
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Screening for quality indicators and phenolic compounds of biotechnological interest in honey samples from six species of stingless bees (Hymenoptera: Apidae) Ciênc. Tecnol. Aliment.
OLIVEIRA,Rosane Gomes de; JAIN,Sona; LUNA,Alexandre Cândido; FREITAS,Lisiane dos Santos; ARAÚJO,Edilson Divino de.
Abstract Honey from stingless bees of the genus Melipona is a well sought product. Nevertheless lack of legal frameworks for quality assessment complicates the evaluation of food safety and marketing of these products. Seeking to assess the quality of honey from the bees of this genus, physical and chemical analyses, identification of phenolic compounds, and microbiological evaluation from six species of stingless bees was performed. The honey samples showed high reducing sugars, low protein levels and a balanced microbiota. High total phenols and flavonoids and higher antioxidant activity were also recorded. Different phenolic compounds of great biotechnological potential were identified and of these apigenin, kaempferol and luteolin were identified for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Honey; Chromatography; Flavonoids; Phenols; Antioxidants; Stingless bees; Pollen types.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000400552
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Honey and bee pollen produced by Meliponini (Apidae) in Alagoas, Brazil: multivariate analysis of physicochemical and antioxidant profiles Ciênc. Tecnol. Aliment.
DUARTE,Alysson Wagner Fernandes; VASCONCELOS,Maria Raphaella dos Santos; ODA-SOUZA,Melissa; OLIVEIRA,Favízia Freitas de; LÓPEZ,Ana Maria Queijeiro.
Abstract This study evidenced the physicochemical composition and antioxidant activity (AA) of honeys (n = 31) and pollen (n = 25) of stingless bees species from Alagoas, Brazil. Fifteen parameters were studied under the light of a multivariate analysis. A dendrogram with three groups of honeys was stablished for the different bees, being the group III formed exclusively by samples from genus Melipona. Plebeia sp. and Tetragona clavipes Fabricius (1804) produced honeys with higher pH/acidity, electrical conductivity, phenolic total and AA. Even different bees from the same meliponary, as M. asilvai and T. clavipes, produced honeys with different AA and chemical profiles, possibly due their different nectar preference. The multivariate analysis of the bee...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melipona; Polyphenols; Flavonoids; Stingless bees; Tetragona clavipes.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000300493
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Diagnosis of meliponiculture in Rio Grande do Sul state (South Brazil) ExtensaoRural
Ambrosini, Larissa Bueno; Kroeff, Denise Reif; Reis, Goreti Ranincheski dos; Sampaio, João Oliveira; Witter, Sídia.
Rational breeding of stingless bees (or meliponines) is called meliponiculture. In Brazil, studies related to meliponiculture are recent, developed with regional bees. The objective of the study was to elaborate a diagnosis of meliponiculture in Rio Grande do Sul. The data were collected in two stages, the first, through a survey carried out with the extension technicians Emater-RS, and the second, with data collected in a statistically significant sample from the beekeepers. The results show that there are 16,209 families involved in the activity, which have stingless bee hives in their establishment; the number of meliponine hives on rural properties in the state is 80,054 thousand, predominantly in the Northwest mesoregion. The profile of beekeepers is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abelhas sem ferrão; Mel; Sustentabilidade ambiental; Stingless bees; Honey; Environmental sustainability.
Ano: 2023 URL: http://periodicos.ufsm.br/extensaorural/article/view/71394
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Karyotypic description of four species of Trigona (Jurine, 1807) (Hymenoptera, Apidae, Meliponini) from the State of Mato Grosso, Brazil Genet. Mol. Biol.
Costa,Kleber França; Brito,Rute Magalhães; Miyazawa,Carlos Suetoshi.
The genus Trigona contains at least 31 species, but there have been few cytogenetic studies of this group. In this work, four species of Trigona (T. branneri, T. chanchamayoensis, T. hyalinata, and T. recursa) from the municipality of Cuiabá, in the State of Mato Grosso, Brazil, were studied. In all of the species, the females had 2n = 34 chromosomes and the males had n = 17. The C-banding patterns showed that the karyotypes of these species consisted mainly of acrocentric and pseudoacrocentric chromosomes. These cytogenetic findings should useful in future phylogenetic studies of this group.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; Karyotype; Meliponini; Stingless bees; Trigona.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200010
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Cytogenetic characterization of Melipona rufiventris Lepeletier 1836 and Melipona mondury Smith 1863 (Hymenoptera, Apidae) by C banding and fluorochromes staining Genet. Mol. Biol.
Lopes,Denilce Meneses; Pompolo,Sílvia das Graças; Campos,Lúcio Antônio de Oliveira; Tavares,Mara Garcia.
The stingless bees Melipona rufiventris and M. mondury were analyzed cytogenetically by conventional staining with Giemsa, C-banding and sequential staining with the fluorochromes CMA3/DA/DAPI. Both species presented 2n = 18 and n = 9, except for one colony of M. rufiventris, in which some individuals had 2n = 19 due to the presence of a B chromosome. After Giemsa staining and C-banding the chromosomes appeared very condensed and presented a high heterochromatic content, making it difficult to localize the centromere and therefore to visualize the chromosomes morphology. The constitutive heterochromatin was located in interstitial chromosome regions covering most of the chromosomes extension and consisted mainly of AT, as shown by DAPI staining. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melipona; Stingless bees; B chromosomes; Fluorochromes; C-banding.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100010
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Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome Genet. Mol. Biol.
Sousa,Cristina Soares de; Serrão,José Eduardo; Bonetti,Ana Maria; Amaral,Isabel Marques Rodrigues; Kerr,Warwick Estevam; Maranhão,Andréa Queiroz; Ueira-Vieira,Carlos.
The insect fat body is a multifunctional organ analogous to the vertebrate liver. The fat body is involved in the metabolism of juvenile hormone, regulation of environmental stress, production of immunity regulator-like proteins in cells and protein storage. However, very little is known about the molecular mechanisms involved in fat body physiology in stingless bees. In this study, we analyzed the transcriptome of the fat body from the stingless bee Melipona scutellaris. In silico analysis of a set of cDNA library sequences yielded 1728 expressed sequence tags (ESTs) and 997 high-quality sequences that were assembled into 29 contigs and 117 singlets. The BLAST X tool showed that 86% of the ESTs shared similarity with Apis mellifera (honeybee) genes. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fat body; Gene expression; Melipona; Stingless bees; Transcriptome.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200022
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