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Optimization of medium composition for transglutaminase production by a Brazilian soil Streptomyces sp. 69
Macedo,Juliana Alves; Sette,Lara Durães; Sato,Hélia Harumi.
Finding a new microbial source of transglutaminase (MTGase) and the study of the medium composition for MTGase production were the goals of this work. A total of 200 actinomycete-like strains were isolated from Brazilian soil samples and two of them named T10b and P20 were selected based on their ability to produce 0.15 U.mL-1 and 0.25 U.mL-1 of MTGase, respectively. Strain P20 was chosen to continue the study and was identified as Streptomyces sp. In order to optimize the MTGase activity, modifications of the usual media composition described for enzyme production were tested. The strategy adopted was: (1) screening experiment for the best carbon and nitrogen sources; (2) fractional factorial design (FFD) to elucidate the key ingredients in the media (the...
Tipo: Journal article Palavras-chave: Central composite design; Fractional factorial design; Medium optimization; Microbial transglutaminase; Streptomyces sp. P20.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400014
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