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A tobacco cDNA reveals two different transcription patterns in vegetative and reproductive organs BJMBR
Silva,I. da; Angelo,P.C.S.; Molfetta,J.B.; Ferraz,M.T.; Silva,L.L.P. da; Goldman,G.H.; Goldman,M.H.S..
In order to identify genes expressed in the pistil that may have a role in the reproduction process, we have established an expressed sequence tags project to randomly sequence clones from a Nicotiana tabacum stigma/style cDNA library. A cDNA clone (MTL-8) showing high sequence similarity to genes encoding glycine-rich RNA-binding proteins was chosen for further characterization. Based on the extensive identity of MTL-8 to the RGP-1a sequence of N. sylvestris, a primer was defined to extend the 5' sequence of MTL-8 by RT-PCR from stigma/style RNAs. The amplification product was sequenced and it was confirmed that MTL-8 corresponds to an mRNA encoding a glycine-rich RNA-binding protein. Two transcripts of different sizes and expression patterns were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine-rich protein; Nicotiana tabacum; Pistil expression; RNA-binding protein; Stress conditions; Virus infection.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000800001
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