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| Baliere, Charlotte; Rince, Alain; Thevenot, Delphine; Gourmelon, Michele. |
| The presence of highly pathogenic Shiga-toxin-producing Escherichia coli (STEC) in shellfish, upstream waters and sediment from coastal shellfish sites was evaluated using the ISO/TS-13136 method. Shellfish (oysters, mussels and cockles), water and sediment samples were collected monthly over a period of 1 year. The method used real-time PCR detection of stx1, stx2 and eae genes and genetic markers corresponding to the five major serogroups (O157, O26, O103, O111 and O145) on enrichment broths and the identification of STEC when these genes and markers were detected. stx genes were detected in the broth of 33% of shellfish batches (n = 126), 91% of water samples (n = 117) and 28% of sediment (n = 39). One stx1+, eae+ O26:H11 strain was isolated from a... |
| Tipo: Text |
Palavras-chave: Eae; Sediment; Shellfish; Shiga-toxin-producing Escherichia coli; Stx; Water. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00250/36124/34975.pdf |
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| Gomes,P.A.D.P.; Bentancor,L.V.; Paccez,J.D.; Sbrogio-Almeida,M.E.; Palermo,M. S.; Ferreira,R.C.C.; Ferreira,L.C.S.. |
| No effective vaccine or immunotherapy is presently available for patients with the hemolytic uremic syndrome (HUS) induced by Shiga-like toxin (Stx) producedbyenterohaemorragic Escherichia coli (EHEC) strains, such as those belonging to the O157:H7 serotype. In this work we evaluated the performance of Bacillus subtilis strains, a harmless spore former gram-positive bacterium species, as a vaccine vehicle for the expression of Stx2B subunit (Stx2B). A recombinant B. subtilis vaccine strain expressing Stx2B under the control of a stress inducible promoter was delivered to BALB/c mice via oral, nasal or subcutaneous routes using both vegetative cells and spores. Mice immunized with vegetative cells by the oral route developed low but specific anti-Stx2B... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EHEC; Stx; Bacillus subtilis; Vaccines; HUS. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200023 |
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