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	Provedor de dados ArchiMer (1) Autor Combes, D (1) Compere, Chantal (1) Delbarre Ladrat, Christine (1) Ghillebaert, F (1) Leroy, Celine (1) Palavra-chave Antifouling (1) Biofilm (1) Capsular protein (1) Exopolymer (1) Pseudoalteromonas (1) Subtilisin (1) Mais... Tipo do documento Text (1) Ano 2008 (1) País France (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Influence of subtilisin on the adhesion of a marine bacterium which produces mainly proteins as extracellular polymers ArchiMer Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Compere, Chantal; Combes, D. Aims: The nature of exopolymers involved in the adhesion of a marine biofilm-forming bacterium Pseudoalteromonas sp. D41 was investigated to evaluate and understand the antifouling potential of subtilisin. Methods and Results: The exopolymers of D41 produced by fermentation were analysed by FTIR and SDS-PAGE showing the presence of polysaccharides, glycoproteins and proteins. A high content of proteins was detected both in soluble and capsular fractions. The microscopic observations of fluorescamine and calcofluor stained adhered D41 indicated mainly the presence of proteins in exopolymers produced during adhesion. Subtilisin, the broad spectrum protease, tested in natural sea water and in polystyrene microplates showed that antifouling activity was... Tipo: Text Palavras-chave: Subtilisin; Pseudoalteromonas; Exopolymer; Capsular protein; Biofilm; Antifouling. Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4624.pdf Registros recuperados: 1 Primeira ... 1 ... Última

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