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	Provedor de dados BABT (2) Ciênc. Tecnol. Aliment. (1) Autor Ananthalakshmy,V. K (1) Gunasekaran,P (1) Gunasekaran,P. (1) Hu,Biao (1) Huang,Kai (1) Kannan,T.R. (1) Raj,K. Chandra (1) Sangiliyandi,G. (1) Yu,Shujuan (1) Zhang,Pingjun (1) Zhang,Xinlin (1) Mais... Palavra-chave Sucrase (3) Levan (2) Levansucrase (2) Calcium chloride (1) In vitro mutagenesis (1) Ion chromatography (1) Polymerase (1) Potassium chloride (1) Selective modulation (1) Zymomonas mobilis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2015 (1) 1999 (2) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Separation of levan-formation and sucrose-hydrolysis catalyzed by levansucrase of Zymomonas mobilis using in vitro mutagenesis BABT Sangiliyandi,G.; Kannan,T.R.; Raj,K. Chandra; Gunasekaran,P.. A levansucrase (SacB) of Zymomonas mobilis capable of sucrose hydrolysis but not levan formation was isolated through invitro mutagenesis of cloned sacB gene. When the sacB mutant gene was expressed in Escherichiacoli strains, only 50% of the sucrose-hydrolysing activity (2.0 U/mg) was produced, compared to the wild type levansucrase (4.0 U/mg). Sequencing of the sacB mutant gene revealed changes of two amino acid residues (Phe-102 to Leu and Trp-261 to Lys in the levansucrase). The absence of mutation at the site of Cys of SacB is contradictory to the inhibition kinetics that demonstrated the involvement of Cys in conferring the levan-forming activity to the SacB. The present finding is useful in understanding the mechanism of selective modulation of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Levansucrase; Polymerase; Sucrase; In vitro mutagenesis; Levan; Selective modulation. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000400001 Optimization of levan production by Zymomonas mobilis BABT Ananthalakshmy,V. K; Gunasekaran,P. Effect of different fermentation conditions on levan production by Zymomonas mobilis B-4286 was studied. Levan production increased from 5.7-g/l to 12.6-g/l with an increase in initial sucrose concentration (50-150 g/l). Above 15% (20 and 25%) sucrose concentration, there was no increase in the biomass. The sucrose hydrolysis and levan production occurred even in the absence of significant growth of cells. Maximum amount of levan was produced (14.5 g/l) at pH 5 and 15 g /l at 25(0)C temperature. At temperature between 35(0)C and 40(0)C, levan production was not detected. Presence of glucose in the medium considerably reduced levan production (2.8 g/l) than fructose 6.7g/l. Tipo: Info:eu-repo/semantics/article Palavras-chave: Levan; Levansucrase; Sucrase; Zymomonas mobilis. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300004 Solubility and Seeded Metastable Zone width of functional sugar L-arabinose Ciênc. Tecnol. Aliment. Hu,Biao; Huang,Kai; Zhang,Xinlin; Zhang,Pingjun; Yu,Shujuan. L-arabinose is widely used in food, medicine, chemistry, and biology fields; however, solubility and seeded metastable zone width (MSZW) of L-arabinose have not been reported in the literature. In this paper, solubility and MSZW of L-arabinose in aqueous solution were determined. Solubility of L-arabinose was measured in the range of 20-68 °C by a conventional equilibrium solubility method and quantitation was determined using the ion chromatography technique. Seeded MSZW was determined in the range of 51-73% by the calorimetric method. The effect of two salts (potassium chloride and calcium chloride) on the solubility and MSZW of L-arabinose were also evaluated. Results showed that both potassium chloride and calcium chloride increased the solubility of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sucrase; Ion chromatography; Potassium chloride; Calcium chloride. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000100051 Registros recuperados: 3 Primeira ... 1 ... Última

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