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Structural features and assessment of zymosan-induced arthritis in rat temporomandibular joint model using sulfated polysaccharide Biological Sciences
Rodrigues, José Ariévilo Gurgel; Universidade Federal do Ceará; Chaves, Hellíada Vasconcelos; Universidade Federal do Ceará; Alves, Kátia de Souza; Universidade Federal do Ceará; Filgueira, Adriano Aguiar; Universidade Federal do Ceará; Bezerra, Mirna Marques; Universidade Federal do Ceará; Benevides, Norma Maria Barros; Universidade Federal do Ceará.
The green seaweed Caulerpa cupressoides var. lycopodium contains three SPs fractions (Cc-SP1, Cc-SP2 and Cc-SP3). Cc-SP1andCc-SP2 had anticoagulant (in vitro), pro- and antithrombotic, antinociceptive and/or anti-inflammatory (in vivo) effects. This study analyzed structural features and the antinociceptive and anti-inflammatory effects of Cc-SP1 on zymosan-induced acute arthritis of the rat temporomandibular joint (TMJ). Cc-SP1 was investigated by infrared technique. Male Wistar rats (200-240 g) received subcutaneously (s.c.) Cc-SP1 1h prior to intra-articular (i.art.) injection of zymosan (2 mg joint-1) or saline (0.9%) into the left TMJ. Mechanical hypernociception was measured by the electronic Von Frey method in the basal and 4h after zymosan...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chlorophyta; Sulfated polymers; Bioactivity; Inflammatory arthropathies.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/19342
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An antithrombin-dependent sulfated polysaccharide isolated from the green alga Caulerpa cupressoides has in vivo anti- and prothrombotic effects Ciência Rural
Rodrigues,José Ariévilo Gurgel; Queiroz,Ismael Nilo Lino de; Quinderé,Ana Luíza Gomes; Vairo,Bruno Cunha; Mourão,Paulo Antônio de Souza; Benevides,Norma Maria Barros.
Red algae sulfated polysaccharides (SPs) have been widely described as anticoagulant and antithrombotic agents; however no description of antithrombotic activity regarding green algae SPs has been reported. Caulerpa cupressoides (Chlorophyta) has three different SPs fractions (SP1, SP2 and SP3). We investigated the effects of SP2 on thrombin activity by antithrombin and in an experimental model of venous thrombosis in rats. The inhibition of thrombin assay was evaluated using antithrombin (AT) in the presence of SP2 and the antithrombotic activity was investigated in rats with thromboplastin as the thrombogenic stimulus. The anticoagulant effects of SP2 are suggested be due to the potentiation of thrombin inhibition by antithrombin (IC50 ~ 10.0µg mL-1) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Green alga; Sulfated polymers; Biological events; Coagulation system; Thrombosis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000400014
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