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| Registros recuperados: 30 | |
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| Degremont, Lionel; Bedier, Edouard; Boudry, Pierre. |
| Response to divergent selection for "high" and "low" survival during the summer period, from July to October, was investigated in juvenile (six-month-old) Pacific oysters, Crassostrea gigas, by producing two sets of progenies in 2002 (Generation 2) and three sets of progenies in 2003 (Generation 3). A strict between-family approach was used and resistance of these selected progenies to summer mortality was assessed in three sites along French coasts, to determine their response to selection and estimate realized heritability of the trait. A significant difference in survival was observed between the "high" and "low" selected groups at all sites for all sets of progenies, indicating a significant genetic component. High realized heritabilities for survival... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Heritability; Juveniles; Summer mortality; Survival; Growth. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6950.pdf |
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| Degremont, Lionel; Boudry, Pierre; Ropert, Michel; Samain, Jean-francois; Bedier, Edouard; Soletchnik, Patrick. |
| This study investigated the effects of age (first or second summer of life) and environment (three study sites on French coasts) on summer mortality in Crassostrea gigas, using three generations of oysters produced during the Morest project. For each generation, two groups of oysters, selected either as 'resistant' (R) or 'susceptible' (S) to summer mortality at the age of 6 months in Rivière d'Auray (RA, South Brittany), were evaluated. In Ronce (Marennes-Oléron Bay) and RA, R oysters had low mortality during their first and second summers, while mortality was higher during the second summer in Baie des Veys (BDV, Normandy). In RA, S oysters exhibited higher mortality during their first summer than during their second, while the opposite result was found... |
| Tipo: Text |
Palavras-chave: Summer mortality; Age; Environment; Genetic; Crassostrea gigas. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6972.pdf |
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| Lambert, Christophe; Soudant, Phillippe; Degremont, Lionel; Delaporte, Maryse; Moal, Jeanne; Boudry, Pierre; Jean, Frédéric; Huvet, Arnaud; Samain, Jean-francois. |
| High variability among individuals is often encountered when hemocyte characteristics are measured in bivalves. Such variability is suspected to result partly from genetic factors. In this study, hemocyte characteristics of six families of Crassostrea gigas were compared by flow cytometry at one sampling date in October 2001. These families were obtained from a nested, half-sibling cross design, and reared from July to October 2001 at three sites distributed along the French Atlantic coast from north to south: Baie des Veys (Normandy), Riviere d'Auray (Brittany) and Ronce (Marennes-oleron Basin, Poitou Charentes). Among the 15 measured hemocyte characteristics, production of reactive oxygen species (ROS) of untreated hemocytes (maintained in filtered... |
| Tipo: Text |
Palavras-chave: Genetic; Flow cytometry; Reactive oxygen species ROS; Adhesion; Hemocyte; Crassostrea gigas; Summer mortality. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3054.pdf |
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| Degremont, Lionel. |
| During the MOREST project (2001–2006), oyster summer mortality was extensively investigated in Crassostrea gigas in France. However, significant increases in magnitude and geographic distribution of summer mortality episodes have been reported since 2008. This paper investigates the response to selection for survival, which was successfully performed between 2001 and 2003, in this new context. It also examines the relationship between the mortality and the three pathogens Vibrio aestuarianus, V. splendidus and the Ostreid herpesvirus 1 (OsHV-1). Three batches of juvenile (6-month-old) Crassostrea gigas were produced in February 2009 and tested in the field in the Marennes-Oléron Bay during summer (August and September) 2009: one control batch, one batch... |
| Tipo: Text |
Palavras-chave: Summer mortality; Crassostrea gigas; Disease resistance; Ostreid herpesvirus; Vibrio sp; Oyster. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00033/14472/11770.pdf |
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| Couty, Angélique. |
| The Japan oyster: Crassostrea gigas have been imported on the french coasts in the seventies and from this time it adapted to this environment. Since, it became the main oyster producted in France. But ,since twenty years C. gigas presents an anormaly and important mortality eah year in the period of summer. The syndrome known as summer mortality, appears particulary in mid-june. This mortality can affected 50% of the culture. Many studies have brought elements in the comprehension of the condition of apparition of this mortalilities. Temperature seems to play an important rule with reproductive maturation. But also, the deep ofwater could be associated to this mass mortality. The study began in march 2003 and characterize more precisely the summer... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Mortalité estivale; Croissance; Maturation; Environnement; Crassostrea gigas; Summer mortality; Growth; Maturation; Environment. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00306/41767/40980.pdf |
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| Soletchnik, Patrick; Lambert, Christian; Costil, Katherine. |
| The purpose of this study is to investigate summer mortality of the cupped oyster, Crassostrea gigas, in relation to culture practices in the traditional oyster production region of Marennes-Oleron (France). Four oyster rearing conditions, varying culture location ("on-" or "off-bottom"), and site depth (65% to 80% daily immersion termed "deep" or 45% to 65%, termed "shallow") were studied to compare biologic performance and maturation status of oysters, in relation to sediment and water column parameters. The most severe mortality occurred in June to July in "on-bottom" reared oysters (25%), as compared with 10% mortality in "off-bottom" cultured oysters. Oysters (shell and meat) grew significantly better when reared "off-bottom" than "on-bottom."... |
| Tipo: Text |
Palavras-chave: Summer mortality; Hydrology; Sediment; Marine ecosystem; Marennes Oleron Bay; Crassostrea gigas. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3171.pdf |
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| Sauvage, Christopher; Boudry, Pierre; De Koning, D. -j.; Haley, C. S.; Heurtebise, Serge; Lapegue, Sylvie. |
| P>Summer mortality is a phenomenon severely affecting the aquaculture production of the Pacific oyster (Crassostrea gigas). Although its causal factors are complex, resistance to mortality has been described as a highly heritable trait, and several pathogens including the virus Ostreid Herpes virus type 1 (OsHV-1) have been associated with this phenomenon. A QTL analysis for survival of summer mortality and OsHV-1 load, estimated using real-time PCR, was performed using five F-2 full-sib families resulting from a divergent selection experiment for resistance to summer mortality. A consensus linkage map was built using 29 SNPs and 51 microsatellite markers. Five significant QTL were identified and assigned to linkage groups V, VI, VII and IX. Analysis of... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Herpes virus; Pacific oyster; QTL mapping; Summer mortality. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00011/12241/9063.pdf |
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| Royer, J; Ropert, Michel; Costil, K. |
| Summer mortalities have been observed in French shellfish areas (including Normandy) since 1994, but origin of this syndrome remains unclear and is suspected to be caused by a combination of several interacting extrinsic (biotic and abiotic) and intrinsic (genetic, physiological, immunological) factors. The French research program, MOREST aimed to identify the origin of oyster summer mortality along the French coast, focusing on the interactions between oysters, their pathogens, and the environment. The present study analyzed spatio-temporal variation in growth, condition and mortality in spat, and half-grown and market-sized oysters reared from February 2000 to October 2003 at six stations within two different areas in the Bay of Veys, Normandy: Grandcamp... |
| Tipo: Text |
Palavras-chave: France English Channel; Growth; Summer mortality; Crassostrea gigas; Oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-4733.pdf |
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| Huvet, Arnaud; Degremont, Lionel; Labreuche, Yannick; Daniel, Jean-yves; Dubrunfaut, Thomas; Boudry, Pierre; Haure, Joel; Bedier, Edouard; Ropert, Michel; Herpin, Amaury; Cunningham, Charles; Moal, Jeanne; Samain, Jean-francois. |
| Summer mortality associated with juveniles of the oyster Crassostrea gigas is the result of a complex interaction between the host, pathogens and environment. Divergent selection based on summer survival have been applied to produce families with resistant and susceptible progeny. Here, we describe the use of suppression subtractive hybridization to generate 150 clones that were differentially regulated between resistant and susceptible F2 progeny. The nucleotide sequence of these 150 clones was determined. Among the 25 unique sequences that matched with the products of known genes, those putatively implicated in energy generation (12%) and immune function (16%), systems suspected to be implicated in summer mortality, were extended and analyzed by real... |
| Tipo: Text |
Palavras-chave: Selection; Gene; Genetic; Summer mortality; Crassostrea gigas; Pacific oysters. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3447.pdf |
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| Normand, Julien; Li, Ronghua; Quillien, Virgile; Nicolas, Jean-louis; Boudry, Pierre; Pernet, Fabrice; Huvet, Arnaud. |
| Pacific oyster Crassostrea gigas suffers from chronic or sporadic mortality outbreaks worldwide, resulting from infectious diseases and/or physiological disorders triggered by environmental factors. Since 2008, ostreid herpesvirus OsHV-1 μVar has been identified as the main agent responsible for mass mortality of juvenile oysters in Europe. Previous studies of genome-wide expression profiling have provided candidate genes that potentially contribute to genetically-based resistance to summer mortality. To assess their value in determining resistance to the juvenile mass mortality that has occurred in France since 2008, we analyzed the expression of 17 candidate genes in an experimental infection by OsHV-1 μVar, and in an in vivo field experiment. Individual... |
| Tipo: Text |
Palavras-chave: Molluscs; Gene expression profiling; Inhibitor of kappa B; Summer mortality; Ostreid herpesvirus. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00177/28823/27325.pdf |
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| Samain, Jean-francois; Ropert, Michel; Bedier, Edouard; Soletchnik, Patrick; Mazurie, Joseph; Le Coz, Florence; Blin, Jean-louis; Mille, Dominique; Pajot, Régis; Haffray, Patrick; Bacher, Cedric; Grangere, Karine; Grangere, Karine; Pouvreau, Stephane; Bourles, Yves; Gohin, Francis; Woerther, Patrice; Boudry, Pierre. |
| Summer mortality has been reported in the Pacific oyster, Crassostrea gigas, for many years in Japan, US and Europe. It affects both adults and juveniles and the causes of this phenomenon are complex. Environmental, physiological or pathological putative causes were studied all over the world. Recently, the MOREST project ((MORtalitEStivale) a multidisciplinary project (2001-2005) focused on his problem led to a better understanding of this phenomenon in France. The results showed in these geographical conditions, the main interactions between the oysters, their environment and certain pathogens. |
| Tipo: Text |
Palavras-chave: Interaction model; Environmental parameters; Summer mortality; Crassostrea gigas; Pacific oyster. |
| Ano: 2006 |
URL: https://archimer.ifremer.fr/doc/2006/acte-3476.pdf |
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| Degremont, Lionel; Soletchnik, Patrick; Boudry, Pierre. |
| This study reports mortality under laboratory conditions in unselected controls and two lines of juvenile Pacific oysters Crassostrea gigas, previously selected for their high or low survival in the field during the summer period. Oysters were also deployed in field conditions and mortality between both conditions was then compared. In the laboratory, mortality was observed in all experiments and it always lasted for a week indicating that mortality under laboratory conditions was a short-term event. It was also shown that mortality could be repeated for a batch in several experiments using oysters which never experienced any abnormal mortality. This approach could facilitate further studies to investigate the causes of mortality by allowing repeated... |
| Tipo: Text |
Palavras-chave: Summer mortality; Crassostrea gigas; Laboratory; Field; Oyster; Juvenile. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00013/12432/9228.pdf |
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| Fleury, Elodie; Huvet, Arnaud. |
| Summer mortality of Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. A high heritability was estimated for resistance to summer mortality, which provided an opportunity to develop lines of oysters that were resistant (R) or susceptible (S) to summer mortality. Previous genome-wide expression profiling study of R and S oyster gonads highlighted reproduction and antioxidant defense as constitutive pathways that operate differentially between these two lines. Here, we show that signaling in innate immunity also operates differentially between these lines, and we hypothesize that this is at the main determinant of their difference in survival in the field. A reanalysis of our published microarray data... |
| Tipo: Text |
Palavras-chave: Mollusca; Crassostrea gigas; Gene; Immunity; CDNA microarray; Summer mortality. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00083/19423/17213.pdf |
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| Huvet, Arnaud; Normand, Julien; Fleury, Elodie; Quillien, Virgile; Fabioux, Caroline; Boudry, Pierre. |
| Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment and their pathogens. The physiological status of an oyster, especially its reproductive status, is suspected to play a significant role in the outcome of this interaction. As genetic variability exists for susceptibility to summer mortality, resistant (R) and susceptible (S) oyster lines were produced using a divergent selection scheme. The present paper reports a histological study on gonad area, which is representative of reproductive effort, in randomly chosen five R and five S oyster lines. The R lines showed a significantly lower gonad area than the S lines (P<0.001). with an estimated mean difference of 12.5%, whereas,... |
| Tipo: Text |
Palavras-chave: Bivalve mollusc; Crassostrea gigas; Reproduction; Selected lines; Summer mortality. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00006/11766/8502.pdf |
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| Delaporte, Maryse; Soudant, P; Lambert, C; Jegaden, M; Moal, Jeanne; Pouvreau, Stephane; Degremont, Lionel; Boudry, Pierre; Samain, Jean-francois. |
| Within the framework of a national scientific program named "MORtalites ESTivales de l'huitre creuse Crassostrea gigas" (MOREST), a family-based experiment was developed to study the genetic basis of resistance to summer mortality in the Pacific oyster, Crassostrea gigas. As part of the MOREST project, the second generation of three resistant families and two susceptible families were chosen and pooled into two respective groups: "R" and "S". These two groups of oysters were conditioned for 6 months on two food levels (4% and 12% of oyster soft-tissue dry weight in algal dry weight per day) with a temperature gradient that mimicked the Marennes-Oleron natural cycle during the oyster reproductive period. Oyster mortality remained low for the first two... |
| Tipo: Text |
Palavras-chave: Summer mortality; Reproduction; Reactive oxygen species ROS; Hemocyte parameters; Genetic selection; Crassostrea gigas. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3578.pdf |
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| Registros recuperados: 30 | |
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