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Boutet, Isabelle; Ky, Chin Long; Bonhomme, Francois. |
Euryhaline teleosts possess the capacity to osmoregulate under various environmental conditions (freshwater to hypersaline water). This physiological capacity is generally monitored using enzyme activity assays (Na+/K+-ATPase...), hormones quantification (prolactine, growth hormone) or their mRNAs expression. To date, few studies addressed the genetic correlates of adaptation to varying salinity at a molecular level in such fish. In the sea bass Dicentrarchus labrax, genetic differentiation was observed at specific allozyme loci between lagoon- and open-sea populations. In the present study, we investigated transcriptomic response of D. labrax to salt- and freshwater acclimation in two organs involved in osmoregulation, gill and intestine. By using... |
Tipo: Text |
Palavras-chave: Suppression subtractive hybridisation; Carbonic anhydrase; Osmoregulation; MRNA expression; Sea bass. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1901.pdf |
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Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Segarra, Amelie; Chollet, Bruno; Renault, Tristan. |
Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms. Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in... |
Tipo: Text |
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Flat oyster; Haemocytes; Gene expression; Suppression subtractive hybridisation. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00018/12879/9828.pdf |
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