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| Lucheta,Adriano R.; Silva-Pinhati,Ana Carla O.; Basílio-Palmieri,Ana Carolina; Berger,Irving J.; Freitas-Astúa,Juliana; Cristofani,Mariângela. |
| Citrus species are known by their high content of phenolic compounds, including a wide range of flavonoids. In plants, these compounds are involved in protection against biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. In humans, flavonoid consumption has been related to increasing overall health and fighting some important diseases. The goals of this study were to identify expressed sequence tags (EST) in Citrus sinensis (L.) Osbeck corresponding to genes involved in general phenylpropanoid biosynthesis and the key genes involved in the main flavonoids pathways (flavanones, flavones, flavonols, leucoanthocyanidins, anthocyanins and isoflavonoids). A thorough analysis of all related putative genes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phenylpropanoids; Sweet orange; Expressed sequence tags (EST); Secondary metabolism. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500010 |
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| Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Targon,Maria Luisa P.N.; Carlos,Eduardo F.; Locali-Fabris,Eliane C.; Amaral,Alexandre M.; Freitas-Astúa,Juliana; Silva-Pinhati,Ana Carla O.; Boscariol-Camargo,Raquel L.; Berger,Irving J.; Rodrigues,Carolina M.; Reis,Marcelo S.; Machado,Marcos A.. |
| In order to understand the genetic responses resulting from physiological changes that occur in plants displaying citrus variegated chlorosis (CVC) symptoms, we adopted a strategy of comparing two EST libraries from sweet orange [Citrus sinensis (L.) Osbeck]. One of them was prepared with plants showing typical CVC symptoms caused by Xylella fastidiosa and the other with non-inoculated plants. We obtained 15,944 ESTs by sequencing the two cDNA libraries. Using an in silico hybridization strategy, 37 genes were found to have significant variation at the transcriptional level. Within this subset, 21 were up-regulated and 16 were down-regulated in plants with CVC. The main functional categories of the down-regulated transcripts in plants with CVC were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sweet orange; EST; CVC; Compatible interaction. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500023 |
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| Freitas-Astúa,Juliana; Bastianel,Marinês; Locali-Fabris,Eliane C.; Novelli,Valdenice M.; Silva-Pinhati,Ana Carla; Basílio-Palmieri,Ana Carolina; Targon,Maria Luisa P.N.; Machado,Marcos A.. |
| Leprosis, caused by Citrus leprosis virus, cytoplasmic type (CiLV-C), is the main viral disease in the Brazilian citrus industry. This occurs because of the widespread source of inoculum and the year-round presence of the vector, the tenuipalpid mite Brevipalpus phoenicis, in citrus plants. In addition, while some Citrus species are resistant to CiLV-C, C. sinensis, the main cultivated species in the country, is extremely susceptible to the disease. The main objective of this work was to identify genes in C. sinensis cv. Pêra plants that were differentially expressed after the host was challenged with CiLV-C. In order to accomplish that, cDNA libraries were constructed from healthy and CiLV-inoculated sweet orange leaves. Two hundred and fifty-four genes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EST; Disease; CiLV; In silico hybridization; Sweet orange; Citrus sinensis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500026 |
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| Bona,Claudine Maria de; Gould,Jean Howe; Miller Jr,J. Creighton; Stelly,David; Louzada,Eliezer Silva. |
| The objective of this study was to produce citrus somatic asymmetric hybrids by fusing gamma-irradiated protoplasts with iodoacetamide-treated protoplasts. Protoplasts were isolated from embryogenic suspension cells of grapefruit (Citrus paradisi Macfad.) cultivars Ruby Red and Flame, sweet oranges (C. sinensis Osbeck) 'Itaboraí', 'Natal', Valencia', and 'Succari', from 'Satsuma' (C. unshiu Marcow.) and 'Changsha' mandarin (C. reticulata Blanco) and 'Murcott' tangor (C. reticulata x C. sinensis). Donor protoplasts were exposed to gamma rays and receptor protoplasts were treated with 3 mmol L-1 iodoacetamide (IOA), and then they were fused for asymmetric hybridization. Asymmetric embryos were germinated, and the resulting shoots were either grafted onto... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus sp.; Gamma irradiation; Grapefruit; Protoplast fusion; Somatic hybridization; Sweet orange. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000500004 |
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