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Registros recuperados: 75
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Cyrtopodium paludicolum germination with two Tulasnella isolates Acta Botanica
Carvalho,Otieres Cirino de; Paiva Neto,Vespasiano Borges de; Padilha,Daly Roxana Castro; Veloso,Tomás Gomes Reis; Bocayuva,Melissa Faust; Soares,Deanna Carla Oliveira; Kasuya,Maria Catarina Megumi.
ABSTRACT Symbiosis between orchid seeds and mycorrhizal fungi has been reported to be a determining factor in the success of germination and protocorm development in vitro. The aim of this study was to isolate and identify by molecular analysis the mycorrhizal fungus associated with Cyrtopodium paludicolum, and to evaluate its efficiency in facilitating seed germination and development. Germination experiments were carried out using a fungus isolated from C. paludicolum (CH01) and Epidendrum secundum (M65), which has been successfully used a number of times in symbiotic germination. The experiments were conducted in a completely randomized design with treatments of CH01, M65 as well as under asymbiotic conditions. The mycobiont CH01 was successfully...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; ITS-DNAr; Mycorrhiza; Orchidaceae; Symbiosis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000100107
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Arbuscular mycorrhiza in species of Commelinidae (Liliopsida) in the state of Pernambuco (Brazil) Acta Botanica
Silva,Gladstone Alves da; Santos,Bartolomeu Acioli dos; Alves,Marccus Vinícius; Maia,Leonor Costa.
Mycorrhiza are a mutualistic symbiosis between fungi and plant roots, the main benefit to the plant being increased nutrient uptake. The arbuscular is the most important kind of mycorrhiza for agriculture and it is widespread in occurrence and distribution in most ecosystems. The aim of this work was to study the mycorrhizal status of the species of Commelinidae that occur in the State of Pernambuco. Plant roots, collected in ten municipalities, were washed, cleared in KOH, stained with Trypan blue in lactoglycerol and observed under a light microscope in order to assess presence and identification of the mycorrhizal type. Percentage of root colonization was evaluated by the gridline intersect method. Forty specimens representing 30 species were observed....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyperaceae; Eriocaulaceae; Poaceae; Mycorrhizal fungi; Symbiosis.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062001000200002
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Symbiotic propagation of seedlings of Cyrtopodium glutiniferum Raddi (Orchidaceae) Acta Botanica
Guimarães,Fernanda Aparecida Rodrigues; Pereira,Marlon Corrêa; Felício,Cristiane da Silva; Torres,Denise Pereira; Oliveira,Sabrina Feliciano; Veloso,Tomás Gomes Reis; Kasuya,Maria Catarina Megumi.
In nature, orchid seeds obtain the nutrients necessary for germination by degrading intracellular fungal structures formed after colonization of the embryo by mycorrhizal fungi. Protocols for asymbiotic germination of orchid seeds typically use media with high concentrations of soluble carbohydrate and minerals. However, when reintroduced into the field, seedlings obtained via asymbiotic germination have lower survival rates than do seedlings obtained via symbiotic germination. Tree fern fiber, the ideal substrate for orchid seedling acclimatization, is increasingly scarce. Here, we evaluated seed germination and protocorm development of Cyrtopodium glutiniferum Raddi cultivated in asymbiotic media (Knudson C and Murashige & Skoog) and in oatmeal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epulorhiza sp.; Symbiosis; Orchid; Substrate; Fern fiber.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000300016
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Characterization of seed germination and protocorm development of Cyrtopodium glutiniferum (Orchidaceae) promoted by mycorrhizal fungi Epulorhiza spp. Acta Botanica
Pereira,Marlon Corrêa; Rocha,Diego Ismael; Veloso,Tomás Gomes Reis; Pereira,Olinto Liparini; Francino,Dayana Maria Teodoro; Meira,Renata Maria Strozi Alves; Kasuya,Maria Catarina Megumi.
Cyrtopodium glutiniferum is an endemic orchid of Brazil with potential medicinal and ornamental applications. As mycorrhizal fungi are essential for the initiation of the orchid life cycle, the aim of this study was to determine the strains of mycorrhizal fungi suitable for seed germination and protocorm development of C. glutiniferum and to characterize the symbiotic development of protocorms. Seeds of C. glutiniferum were inoculated with nine mycorrhizal fungi, Epulorhiza spp., Ceratorhiza spp., Rhizoctonia sp., originally isolated from Brazilian neotropical orchids. Only Epulorhiza isolates promoted seed germination and protocorm development. Three Epulorhiza isolates (M1, M6 = E. epiphytica, M20 = Epulorhiza sp.) promoted protocorm development until...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ceratorhiza; Epulorhiza; Orchid conservation; Seedling production; Symbiosis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400567
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Development beyond the gastrula stage and digestive organogenesis in the apple-snail Pomacea canaliculata (Architaenioglossa, Ampullariidae) Biocell
Koch,E.; Winik,B.C.; Castro-Vazquez,A..
Development of Pomacea canaliculata from the gastrula stage until the first day after hatching is described. Trochophore embryos are developed after gastrulation, showing the prototroch as a crown of ciliated orange-brownish cells. However, no true veliger embryos are formed, since the prototroch does not fully develop into a velum. Afterward, the connection between the fore- and midgut is permeated and the midgut becomes full of the pink-reddish albumen, which is stored into a central archenteron's lake, from where it is accumulated into the large cells forming the midgut wall ("giant cells"). Electron microscopy of giant cells in late embryos showed that albumen is engulfed by large endocytic vesicles formed between the irregular microvilli at the top of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alimentary canal; Midgut gland; Symbiosis; Albumen.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100006
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A study of chlorophyll-like and phycobilin pigments in the C endosymbiont of the apple- snail Pomacea canaliculata Biocell
Vega,Israel A.; Dellagnola,Federico A.; Hurst,Jorge A.; Godoy,Martín S.; Castro-Vazquez,Alfredo.
Pigments present in the brown-greenish C morph of an intracellular endosymbiont of Pomacea canaliculata were investigated. Acetone extracts of the endosymbiotic corpuscles showed an absorption spectrum similar to that of chlorophylls. Three fractions obtained from silica gel column chromatography of the acetone extracts (C I, C II and C III), were studied by positive ion fast atom bombardment-mass spectrometry (FAB-MS) and hydrogen-nuclear magnetic resonance (H-NMR). Results indicated the presence of (1) a sterol in the yellow colored C I fraction; (2) a mixture of pheophorbides a and b in the major green fraction, C II; and (3) a modified pheophorbide a in the smaller green fraction, C III. Aqueous extracts of the C endosymbiont did not show evidence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Symbiosis; Cyanobacteria; Chloroplast; Photosynthetic pigments; Phylogeny.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000200001
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Variation in worm assemblages associated with Pomacea canaliculata (Caenogastropoda, Ampullariidae) in sites near the Río de la Plata estuary, Argentina Biocell
Damborenea,C.; Brusa,F.; Paola,A..
Pomacea canaliculata is a common gastropod in freshwater habitats from Central and Northern Argentina, extending northwards into the Amazon basin. Several Platyhelminthes have been reported associated to P. canaliculata, sharing an intimate relationship with this gastropod host. The objectives of this study were to describe the symbiotic species assemblages associated to P. canaliculata in the study area, and to disclose differences among them. Samples were taken in three typical small streams and one artificial lentic lagoon, all connected with the Río de la Plata estuary. The 81.53% were infested with different symbiotic (sensu lato) species. Among the Platyhelminthes, the commensal Temnocephala iheringi Haswell, 1893 was highly prevalent in all samples,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apple-snails; Platyhelminthes; Trematoda; Annelida; Hirudinea; Symbiosis; Commensalism; Parasitism.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000300006
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New species of Temnocephala (Platyhelminthes, Temnocephalida) ectosymbiont on vulnerable species of aeglids (Crustacea, Anomura) from the Neotropical Region Biota Neotropica
Seixas,Samantha Alves; Dametto,Norton; Périco,Eduardo.
Abstract: A new species of the genus Temnocephala Blanchard, 1849 from southern Brazil was found on two species of anomuran crustaceans, Aegla spinipalma Bond-Buckup & Buckup, 1994 and Aegla grisella Bond-Buckup & Buckup, 1994, the latter classified as a vulnerable species by the "Lista de Referência da Fauna Ameaçada de Extinção no Rio Grande do Sul. Decreto no 41.672, de 11 junho de 2002". The crustaceans were collected from a tributary creek of the Forqueta river, Perau de Janeiro, Arvorezinha and a tributary creek of the Fão river, Pouso Novo, Rio Grande do Sul, Brazil; both localities belong to the Sub-Basin of Forqueta River. The new species differs from seven other temnocephalans epibionts on Aegla Leach, 1820, by having the following...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crustacean; Ectosymbiont; South America; Symbiosis; Taxonomy.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032018000400203
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Endophytic fungi from medicinal plant Bauhinia forficata: Diversity and biotechnological potential BJM
Bezerra,Jadson D.P.; Nascimento,Carlos C.F.; Barbosa,Renan do N.; Silva,Dianny C.V. da; Svedese,Virgínia M.; Silva-Nogueira,Eliane B.; Gomes,Bruno S.; Paiva,Laura M.; Souza-Motta,Cristina M..
Bauhinia forficata is native to South America and used with relative success in the folk medicine in Brazil. The diversity, antibacterial activity, and extracellular hydrolytic enzymes of endophytic fungi associated with this plant were studied. Plant samples, which included leaves, sepals, stems, and seeds, were used. Ninety-five endophytic fungal were isolated (18 from leaves, 22 from sepals, 46 from stems, and nine from seeds), comprising 28 species. The most frequently isolated species were Acremonium curvulum (9.5%), Aspergillus ochraceus (7.37%), Gibberella fujikuroi (10.53%), Myrothecium verrucaria (10.53%) and Trichoderma piluliferum(7.37%). Diversity and species richness were higher in stem tissues, and Sorensen’s index of similarity between the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial agents; Fungal endophytes; Hydrolytic enzyme; Symbiosis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100049
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Genome sequence of Bradyrhizobium embrapense strain CNPSo 2833T, isolated from a root nodule of Desmodium heterocarpon BJM
Delamuta,Jakeline Renata Marçon; Ribeiro,Renan Augusto; Gomes,Douglas Fabiano; Souza,Renata Carolini; Chueire,Ligia Maria Oliveira; Hungria,Mariangela.
Abstract Bradyrhizobium embrapense CNPSo 2833T is a nitrogen-fixing symbiont of the legume pasture Desmodium. Its draft genome contains 8,267,832 bp and 7876 CDSs. The symbiotic island includes nodulation and nitrogen fixation genes resembling the operon organization of B. japonicum. Several CDSs related to secretion proteins and stress tolerance were also identified.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological nitrogen fixation; Genome; Nodulation; Symbiosis.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100009
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Respuesta de la simbiosis tripartita Rhizobium-Leguminosa-Micorriza arbuscular ante vanadio y níquel. Colegio de Postgraduados
Vidal Fernández, Judith Estela.
Este trabajo evaluó la respuesta de la simbiosis tripartita Rhizobium-leguminosa-micorriza arbuscular en presencia de vanadio (V) y níquel (Ni). El trabajo se dividió en dos fases experimentales; la primera fase estudió el comportamiento de la simbiosis Rhizobium tropici-Phaseolus vulgaris ante concentraciones de V o Ni (0, 25, 50, 75, 100 y 120 mg L-1) y su efecto en el contenido elemental en nódulos, en un sistema de bolsa de crecimiento bajo condiciones controladas (25°C, 70% HR y 12h de fotoperiodo) durante 30 días. En las plantas con V, la nodulación inició al cuarto día después de la inoculación y tuvo un máximo de 40 nódulos por planta en el testigo y 6 nódulos en las concentraciones de 50 y 100 mg L-1. La mayor actividad nitrogenasa (AN) se...
Palavras-chave: Nodulación; Metales; Fitorremediación; Simbiosis; Phaseolus; Rhizophagus; Nodulation; Metals; Phytorremediation; Symbiosis; Edafología; Maestría.
Ano: 2012 URL: http://hdl.handle.net/10521/753
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Casuarina equisetifolia fitorremeciación de suelo contaminado con diesel y aplicación de bioestimulación y bioaumentación. Colegio de Postgraduados
Díaz Martínez, María Esther.
En este trabajo se evaluó la germinación de semillas y el crecimiento de plantas de C. equisetifolia L. ante diesel, y su potencial de fitorremediación en combinación de bioaumentación y bioestimulación. La primera fase experimental evaluó el efecto tóxico de 15 dosis de diesel (0, 100, 250, 500, 800, 1000, 1500, 2000, 2500, 3000, 5000, 7500, 10000, 15000 y 20000 mg kg-1) en la germinación de semillas y la emergencia de plántulas. A los 10 días, la germinación fue mayor en concentraciones altas de diesel; la mayor geminación ocurrió en 20000 mg kg-1, y la menor se registró ante 5000 mg kg-1. La emergencia de plántulas fue mayor en las concentraciones menores a 5000 mg kg-1. A los 15 días, el crecimiento de las plántulas disminuyó ante concentraciones...
Palavras-chave: Germinación; Emergencia de plantas; Simbiosis; Fertilización; Microorganismos; Degradación de hidrocarburos; Seed germination; Seedling emergence; Symbiosis; Fertilization; Microorganisms; Hydrocarbon degradation; Maestría; Edafología.
Ano: 2012 URL: http://hdl.handle.net/10521/730
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Crecimiento de cuatro cepas del género Rhizobium en presencia de hidrocarburos policíclicos aromáticos (HPA). Colegio de Postgraduados
González Paredes, Yessica.
Este estudio evaluó el crecimiento de cuatro cepas de Rhizobium en medio contaminado con fenantreno (PHE) o benzo[a]pireno (BaP) en concentraciones de 0, 10, 20, 40, 60, 80 y 100 µg mL-1. En la primera fase experimental, R. tropici CIAT899, R. tropici PRF81, R. leucaena CFN299 y R. etli CFN42 crecieron en condiciones in vitro o en simbiosis con Phaseolus vulgaris L. en presencia de PHE y BaP. El crecimiento in vitro de las cuatro cepas a las 24 h, disminuyó al aumentar las concentraciones de ambos HPA; sin embargo, el BaP fue más tóxico para su crecimiento. Las cuatro cepas ante 80 y 100 µg BaP mL-1 presentaron una fase de adaptación; para CIAT899 y PRF81 esta fase fue de 48 h, y para CFN299 y CFN42 esta fase fue de 72 y 96 h, respectivamente. La simbiosis...
Palavras-chave: Rizobios; Tolerancia; Adaptación; Simbiosis; Degradación; Fenantreno; Benzol [a] pireno; Rhizobia; Tolerance; Adaptation; Symbiosis; Degradation; Phenanthrene; Benzol [a] pyrene; Edafología; Maestría.
Ano: 2012 URL: http://hdl.handle.net/10521/1704
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Holobionts and their hologenomes: Evolution with mixed modes of inheritance Genet. Mol. Biol.
Haag,Karen Luisa.
Abstract Symbioses are ubiquitous and have played an influential role in the evolution of life on Earth. Genomic studies are now revealing a huge diversity of associations among hosts and their microbiotas, allowing us to characterize their complex ecological and evolutionary dynamics. The different transmission modes and the asynchronous cell proliferation of the numerous symbionts associated with one host generate a genomic conflict ought to be solved. Two disputing views have been used to model and predict the outcome of such conflicts. The traditional view is based on community ecology, and considers that selection at the level of individuals is sufficient to explain longstanding associations among species. A new perspective considers that the host and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genomics; Metagenomics; Microbiota; Symbiosis; Holobiont.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200189
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A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots Genet. Mol. Biol.
Rodrigues,Elisete Pains; Torres,Adalgisa Ribeiro; Batista,Jesiane Stefânia da Silva; Huergo,Luciano; Hungria,Mariangela.
Sample preparation is a critical step in two-dimensional gel electrophoresis (2-DE) of plant tissues. Here we describe a phenol/SDS procedure that, although greatly simplified, produced well-resolved and reproducible 2-DE profiles of protein extracts from soybean [Glycine max (L.) Merril] roots. Extractions were made in three replicates using both the original and simplified procedure. To evaluate the quality of the extracted proteins, ten spots were randomly selected and identified by mass spectrometry (MS). The 2-DE gels were equally well resolved, with no streaks or smears, and no significant differences were observed in protein yield, reproducibility, resolution or number of spots. Mass spectra of the ten selected spots were compared with database...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-DE; Protein extraction; Proteomics; Soybean roots; Symbiosis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200016
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Population dynamics of the pea crab Austinixa aidae (Brachyura, Pinnotheridae): a symbiotic of the ghost shrimp Callichirus major (Thalassinidea, Callianassidae) from the southwestern Atlantic Iheringia, Sér. Zool.
Peiró,Douglas F; Mantelatto,Fernando L.
The Pinnotheridae family is one of the most diverse and complex groups of brachyuran crabs, many of them symbionts of a wide variety of invertebrates. The present study describes the population dynamics of the pea crab Austinixa aidae (Righi, 1967), a symbiont associated with the burrows of the ghost shrimp Callichirus major (Say, 1818). Individuals (n = 588) were collected bimonthly from May, 2005 to September, 2006 along a sandy beach in the southwestern Atlantic, state of São Paulo, Brazil. Our data indicated that the population demography of A. aidae was characterized by a bimodal size-frequency distribution (between 2.0 and 4.0 mm and between 8.0 and 9.0 mm CW) that remained similar throughout the study period. Sex ratio does not differ significantly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Population structure; Pinnotherids; Intertidal zone; Symbiosis; Brazil.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212011000100001
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Benefícios da adubação verde sobre a simbiose micorrízica e a produtividade de batata-doce. Infoteca-e
ESPÍNDOLA, J. A. A.; ALMEIDA, D. L. de; GUERRA, J. G. M..
Plantio das leguminosas e da batata doce. Produtividade da batata doce e simbiose micorrízica.
Tipo: Folhetos Palavras-chave: Ipomoea batata; Mycorrhiza; Production; Adubação Verde; Batata Doce; Legume; Leguminosa; Micorriza; Produção; Plantio; Simbiose; Green manures; Mycorrhizae; Symbiosis; Sweet potatoes.
Ano: 1997 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/623563
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Interação de micorriza vesicular-arbuscular com leguminosas em solos ácidos. Infoteca-e
LOCATELLI, M.; SMYTH, T. J.; VIEIRA, A. H..
Este trabalho apresenta uma abordagem para MVA e acidez do solo; Simbiose MVA ? Rhizobium-leguminosa; Dependência de MVA e Micorrizas e pesquisa na região amazônica.
Tipo: Folhetos Palavras-chave: Crescimento da planta; Leguminosa; Simbiose; Raiz; Fungo; Micorriza Vesicular Arbuscular; Nutrição Vegetal; Acidez do Solo; Plant nutrition; Soil pH; Legumes; Plant growth; Symbiosis; Vesicular arbuscular mycorrhizae.
Ano: 2001 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/703811
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Micorrizas: da associação mutualística com algodoeiro aos benefícios. Infoteca-e
COSTA, M. M. M. N..
Devido aos problemas supracitados trazidos pela Revolução Verde, foi necessário uma nova mudança de paradigmas na produção agropecuária. Particularmente no que se refere à fertilidade do solo e à nutrição da planta, foi preciso mudar o foco das pesquisas científicas, uma vez que corretivos e adubos solúveis traziam consigo a ideia de ‘alimentar a planta’ com elementos minerais, sendo o solo um mero suporte mecânico para as raízes. Dessa forma, para manter a sustentabilidade da produção vegetal, era necessário um novo conceito, pautado em ‘construção do solo’, com insumos que melhorassem a longo prazo as características químicas, físicas e biológicas do solo, a exemplo de adubação orgânica, adubação verde, bactérias fixadoras de nitrogênio atmosférico e...
Tipo: Folhetos Palavras-chave: Nutrição de planta; Soil microbiology; Algodão; Fungo; Raiz; Micorriza; Absorção de Água; Absorção de Nutrientes; Microbiologia do Solo; Nutrição Vegetal; Cotton; Fungus physiology; Plant nutrition; Symbiosis; Mycorrhizal fungi; Microbiology.
Ano: 2024 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1164118
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"Beta-rizóbios": os novos simbiontes encontrados em espécies de Mimosa. Infoteca-e
REIS JÚNIOR, F. B. dos; FARIA, S. M. de; MENDES, I. de C.; SIMON, M. F.; LOUREIRO, M. de F.; ELLIOT, G. N.; YOUNG, P.; SPRENT, J. I.; JAMES, E. K..
The fixation and transformation of atmospheric dinitrogen into ammonia and hence into organic e compounds is a crucial part of the nitrogen cycle. Nitrogenase, the enzyme responsible, has a wide taxonomie distribution in the bacteria and archaea, but is confined to a limited number of species. Among these, rhizobia, whieh form nodules on the roots of legumes, are recognised as the main contributors of biologieally-fixed nitrogen. Until recently, it has been generally accepted that legumes are nodulated exelusively by members of the alfa-proteobaeteria belonging to a number of related genera in the family Rhizobiaceae. In this document we review some results from recent studies that presents the newly-discovered beta-rhizobia which challenge the accepted...
Tipo: Folhetos Palavras-chave: Feed legumes; Fixação de Nitrogênio; Leguminosa Forrageira; Simbiose; Rhizobium; Nitrogen fixation; Symbiosis.
Ano: 2006 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/570271
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