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	Provedor de dados Electron. J. Biotechnol. (2) BABT (1) Autor Cavalitto,Sebastián Fernando (1) Dagbagli,Seval (1) Goksungur,Yekta (1) Hours,Roque Alberto (1) Lopes,Carlos Edison (1) Mignone,Carlos Fernando (1) Pereira Júnior,Nei (1) Queiroz Sousa,Maria de Fátima Vieira de (1) Rojas,Natalia Lorena (1) Mais... Palavra-chave Synthetic medium (3) Actinomycin D (1) B-galactosidase (1) Carbon balance (1) Enzymatic pectin extraction (1) Kluyveromyces lactis (1) Microbial pectin utilization (1) Pectinases (1) Production (1) Response surface methodology (1) Shake flask culture (1) Streptomyces. parvulus (1) Mais... Tipo do documento Journal article (2) Info:eu-repo/semantics/article (1) Ano 2008 (2) 2001 (1) País Chile (2) Brazil (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última A chemically defined medium for production of actinomycin D by Streptomyces parvulus BABT Queiroz Sousa,Maria de Fátima Vieira de; Lopes,Carlos Edison; Pereira Júnior,Nei. A chemically defined medium consisting of D(+)fructose, L(-)threonine, K2HPO4, MgSO4.7H2O, ZnSO4.7H2O, CaCl2.2H2O, FeSO4.7H2O and deionized water, was developed to maximize the synthesis of actinomycin D by the Streptomyces parvulus DAUFPE 3124 strain. This medium resulted in the maximum antibiotic concentration of 133mg/L while using the original medium the production of actinomycin D was poor not surpassing 43mg/L. Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinomycin D; Production; Streptomyces. parvulus; Synthetic medium. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300002 Optimization of b-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology Electron. J. Biotechnol. Dagbagli,Seval; Goksungur,Yekta. This paper investigates the production and optimization of b-galactosidase enzyme using synthetic medium by Kluyveromyces lactis NRRL Y-8279 in shake flask cultures. Among the different cell disintegration methods used, the highest specific activity was obtained when the cells were permeabilized using isoamyl alcohol. Response surface methodology was used to investigate the effects of four fermentation parameters (agitation speed, pH, initial substrate concentration and incubation time) on b-galactosidase enzyme production. Results of the statistical analysis showed that the fit of the model was good in all cases. Maximum specific enzyme activity of 4218.4 U g-1 was obtained at the optimum levels of process variables (pH 7.35, agitation speed 179.2 rpm,... Tipo: Journal article Palavras-chave: B-galactosidase; Kluyveromyces lactis; Response surface methodology; Shake flask culture; Synthetic medium. Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400011 Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin Electron. J. Biotechnol. Rojas,Natalia Lorena; Cavalitto,Sebastián Fernando; Mignone,Carlos Fernando; Hours,Roque Alberto. Protopectinases (PPases) constitute a heterogeneous group of extracellular enzymes able to release soluble pectin from insoluble protopectin in plant tissues. Geotrichum klebahnii (ATCC 42397) produces PPase-SE with endopolygalacturonase activity. PPase-SE has been used for pectin extraction and maceration of plant tissues. Here, the capacity of G. klebahnii to use different pectins as carbon and energy sources (CES) was studied, in addition to PPase-SE capacity to release pectin from lemon peel. The strain was unable to use pectin from different origins as CES. When G. klebahnii was cultivated with mixtures of different amounts of glucose and citrus pectin as CES, the biomass obtained was proportional to the initial concentration of glucose, which was... Tipo: Journal article Palavras-chave: Carbon balance; Enzymatic pectin extraction; Microbial pectin utilization; Pectinases; Synthetic medium. Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100012 Registros recuperados: 3 Primeira ... 1 ... Última

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