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| Thekisoe, Oriel M. M.; Kuboki, Noritaka; Nambota, Andrew; Fujisaki, Kozo; Sugimoto, Chihiro; Igarashi, Ikuo; Yasuda, Jun; Inoue, Noboru. |
| In this study, we developed loop-mediated isothermal amplification (LAMP) for the specific detection of both animal and human trypanosomosis using primer sets that are designed from 5.8S rRNA-internal transcribed spacer 2 (ITS2) gene for Trypanosoma brucei gambiense, 18S rRNA for both T. congolense and T. cruzi, and VSG RoTat 1.2 for T. evansi. These LAMP primer sets are highly sensitive and are capable of detecting down to 1 fg trypanosomal DNA, which is equivalent to 0.01 trypanosomes. LAMP is a rapid and simple technique since it can be carried out in 1 h and requires only a simple heating device for incubation. Therefore, LAMP has great potential of being used for diagnosis of trypanosomosis in the laboratory and the field, especially in countries that... |
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Palavras-chave: LAMP; Trypanosomosis; Trypanosoma brucei brucei; T. b. rhodesiense; T. b. gambiense; T. congolense; T. cruzi; T. evansi. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1045 |
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| Ndungu, K; Kagira, J.M; Ngotho, J.M; Ouma, J; Bett, B; Gitonga, P. |
| Clinical and pathological characterisation of blood stream (BSF) and cerebrospinal fluid (CSF) forms of Trypanosoma brucei rhodesiense trypanosome isolated from a sleeping sickness patient were investigated in rabbits. The study aimed at investigating whether there is any significant difference in clinical and pathological presentation in rabbits infected by the two forms of trypanosomes. Each form of parasite was inoculated into five rabbits at 104 trypanosomes/ml while five rabbits were used as un-infected controls. Parasitaemia development, body temperature, packed cell volume (PCV), body weight, food and water intake, heartbeat and respiration were monitored daily for 30 days post infection when the experiment was terminated. Pathological changes were... |
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Palavras-chave: Pathogenicity; Rabbits; T. b. rhodesiense; CSF and Blood stream forms. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2387 |
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