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| FUNARI,Cristiano S.; ALMEIDA,Letícia de; PASSALACQUA,Thais G.; MARTINEZ,Isabel; AMBRÓSIO,Daniela L.; CICARELLI,Regina Maria B.; SILVA,Dulce Helena S.; GRAMINHA,Márcia A. S.. |
| ABSTRACT Leishmaniasis and Chagas disease affect millions of people in tropical and subtropical regions. Drugs used currently to treat such diseases often present undesirable side effects and low efficiency. The aim of this work was to identify extracts and isolated compounds from the genus Lippia with leishmanicidal and trypanocidal activity. Fifteen extracts from different plant parts of Lippia species with partially known chemical compositions, four partition fractions, six compounds and a mixture of four interconverting flavanones previously isolated from Lippia salviaefolia and Lippia lupulina were assayed in vitro towards epimastigote forms of Trypanosoma cruzi and promastigote forms of Leishmania amazonensis. The root extract of L. lupulina had... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmanicidal; Trypanocidal; Triterpenoids; Flavonoids; T. cruzi. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672016000400411 |
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| FONSECA,LEONARDO M.; GARCEZ,TATIANA C.; PENHA,LUCIANA; FREIRE-DE-LIMA,LEONARDO; MAES,EMMANUEL; COSTA,KELLI M.; MENDONÇA-PREVIATO,LUCIA; PREVIATO,JOSE O.. |
| ABSTRACT One of the main obstacles to the treatment of Chagas disease is the genetic and phenotypical variance displayed by T. cruzi strains, resulting in differences in morphology, virulence, pathogenicity and drug susceptibility. To better understand the role of glycoconjungates in Chagas disease, we performed the molecular characterization of the O-linked chains from mucins and glycoinositolphospholipids (GIPLs) of the Silvio X10 clone 1 strain. We demonstrated the presence of a β-galactofuranose (β-Galf) unity linked to the O-4 position of the α-N-acetylglucosamine (α-GlcNAc)O-4 in Tc-mucins. GIPLs analysis showed that the lipidic portion is exclusively composed of ceramide and the PI-oligossacharidic portion contains the Man4(AEP)GlcN-Ins-PO4 core,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: T. cruzi; Glycans; Mucins; Glycoinositolphospholipids.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401519 |
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| Toledo,Victor; Ramírez,Galia; Valck,Carolina; López,Nandy; Ribeiro,Carolina H; Maldonado,Ismael; Aguilar,Lorena; Lemus,David; Ferreira,Arturo. |
| Angiogenesis is a complex multi-step process of neovascularization arising from preexisting blood vessels whose generation is regulated by pro- and anti-angiogenic factors. Both Trypanosoma cruzi calreticulin (TcCRT) and its human counterpart (HuCRT) are antiangiogenic. This is the first report where the TcCRT and HuCRT anti-angiogenic properties are compared in vivo. In the chick embryonic chorioallantoid membrane assay (CAM) and at equimolar concentrations, TcCRT displayed significantly higher antiangiogenic activities than its human counterpart. LPS had marginal effects at the concentrations present in the recombinant protein preparations and the TcCRT antiangiogenic effects were largely inhibited by specific polyclonal antibodies, thus, reinforcing the... |
| Tipo: Journal article |
Palavras-chave: Calreticulin; T. cruzi; Anti-angiogenesis. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300004 |
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| Didoli,G.; Revelli,S.; Davila,H.; Ferro,M.E.; Romero-Piffiguer,M.; Bottasso,O.. |
| We demonstrated that administration of interferon gamma (IFN-<FONT FACE="Symbol">g</font>) to the inbred "l" strain of pregnant rats conferred partial resistance on their offspring to challenge with Trypanosoma cruzi. We now examine if this intervention also modifies the reportedly immunodepressed cellular responses which occur during chronic infection. Offspring were born to mothers undergoing one of the following procedures during gestation: subcutaneous injections of recombinant rat IFN-<FONT FACE="Symbol">g</font>, 50,000 IU/rat, five times/week for 3 weeks, which was started on the day of mating (IFN-Mo); infection with 106 trypomastigotes of T. cruzi at 7, 14, and 21 days after mating plus IFN-<FONT... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Interferon gamma; Adjuvant arthritis; T. cruzi; T cell subsets. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600011 |
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| Torres-Silva,Carolina Furtado; Repolês,Bruno Marçal; Ornelas,Hugo Oliveira; Macedo,Andréa Mara; Franco,Glória Regina; Junho Pena,Sérgio Danilo; Tahara,Erich Birelli; Machado,Carlos Renato. |
| Abstract Trypanosoma cruzi is the etiological agent of Chagas disease, a public health challenge due to its morbidity and mortality rates, which affects around 6-7 million people worldwide. Symptoms, response to chemotherapy, and the course of Chagas disease are greatly influenced by T. cruzi‘s intra-specific variability. Thus, DNA mutations in this parasite possibly play a key role in the wide range of clinical manifestations and in drug sensitivity. Indeed, the environmental conditions of oxidative stress faced by T. cruzi during its life cycle can generate genetic mutations. However, the lack of an established experimental design to assess mutation rates in T. cruzi precludes the study of conditions and mechanisms that potentially produce genomic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: T. cruzi; DNA; Mutation frequency; H2O2. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300466 |
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| Thekisoe, Oriel M. M.; Kuboki, Noritaka; Nambota, Andrew; Fujisaki, Kozo; Sugimoto, Chihiro; Igarashi, Ikuo; Yasuda, Jun; Inoue, Noboru. |
| In this study, we developed loop-mediated isothermal amplification (LAMP) for the specific detection of both animal and human trypanosomosis using primer sets that are designed from 5.8S rRNA-internal transcribed spacer 2 (ITS2) gene for Trypanosoma brucei gambiense, 18S rRNA for both T. congolense and T. cruzi, and VSG RoTat 1.2 for T. evansi. These LAMP primer sets are highly sensitive and are capable of detecting down to 1 fg trypanosomal DNA, which is equivalent to 0.01 trypanosomes. LAMP is a rapid and simple technique since it can be carried out in 1 h and requires only a simple heating device for incubation. Therefore, LAMP has great potential of being used for diagnosis of trypanosomosis in the laboratory and the field, especially in countries that... |
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Palavras-chave: LAMP; Trypanosomosis; Trypanosoma brucei brucei; T. b. rhodesiense; T. b. gambiense; T. congolense; T. cruzi; T. evansi. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1045 |
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