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| Salman, Doaa; Okuda, Liria Hiromi; Ueno, Akio; Dautu, George; Zhang, Feifei; Igarashi, Makoto. |
| Oocyst stage of Toxoplasma gondii is characterized by a durable wall that confers a strong protection to this protozoan parasite in face of harsh environmental conditions. Thus, it is considered the key for transmission of T. gondii. Analysis of oocyst wall composition is mandatory therefore; the aim of this study was to identify novel T. gondii oocyst wall proteins and test their use in detection of these oocysts in environmental samples. Five candidates of novel T. gondii oocyst wall proteins (TgOWPs) were identified and named TgOWP8 through TgOWP12. Recombinant protein of TgOWP8 was expressed in E. coli using glutathione S-transferase as fusion protein. Polyclonal antibody was produced and validated by indirect immunofluorescence antibody assay (IFA).... |
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Palavras-chave: T. gondii oocyst wall protein; Toxoplasma gondii. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4541 |
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