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| Traipe-Castro,Leonidas; Salinas-Toro,Daniela; López,Daniela; Zanolli,Mario; Srur,Miguel; Valenzuela,Felipe; Cáceres,Aníbal; Toledo-Araya,Héctor; López-Solís,Remigio. |
| BACKGROUND: Fern-like crystalloids form when a microvolume of tear is allowed to dry out at ambient conditions on a glass surface. Presence of crystalloids in tear "microdesiccates" is used to evaluate patients with Dry-Eye disease. This study aims to examine morphologically the desiccation process of normal tear fluid and to identify changes associated with accelerated tear evaporation. Tear microdesiccates from healthy (Non-Dry Eye) and Dry Eye subjects were produced at ambient conditions. Microdesiccate formation was monitored continuously by dark-field video microscopy. Additionally, accelerated desiccation of tear samples from healthy subjects was conducted under controlled experimental conditions. Particular morphological domains of tear... |
| Tipo: Journal article |
Palavras-chave: Tear; Tear ferning test; Dry eye; Dark-field microscopy. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100025 |
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| Traipe-Salas,Felipe; Traipe-Castro,Leonidas; Salinas-Toro,Daniela; López,Daniela; Valenzuela,Felipe; Cartes,Christian; Toledo-Araya,Héctor; Pérez,Claudio; Solís,Remigio López. |
| BACKGROUND: Tear desiccation on a glass surface followed by transmitted-light microscopy has served as diagnostic test for dry eye. Four distinctive morphological domains (zones I, II, III and transition band) have been recently recognized in tear microdesiccates. Physicochemical dissimilarities among those domains hamper comprehensive microscopic examination of tear microdesiccates. Optimal observation conditions of entire tear microdesiccates are now investigated. One-μ! aliquots of tear collected from individual healthy eyes were dried at ambient conditions on microscope slides. Tear microdesiccates were examined by combining low-magnification objective lenses with transmitted-light microscopy (brightfield, phase contrasts Ph1,2,3 and... |
| Tipo: Journal article |
Palavras-chave: Tear; Tear ferning test; Transmitted-light microscopy; Tear microdesiccate; Dry eye. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100028 |
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