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| Cheng,Fei; Hou,Lin; Woeste,Keith; Shang,Zhengchun; Peng,Xiaobang; Zhao,Peng; Zhang,Shuoxin. |
| Abstract Humic substances in soil DNA samples can influence the assessment of microbial diversity and community composition. Using multiple steps during or after cell lysis adds expenses, is time-consuming, and causes DNA loss. A pretreatment of soil samples and a single step DNA extraction may improve experimental results. In order to optimize a protocol for obtaining high purity DNA from soil microbiota, five prewashing agents were compared in terms of their efficiency and effectiveness in removing soil contaminants. Residual contaminants were precipitated by adding 0.6 mL of 0.5 M CaCl2. Four cell lysis methods were applied to test their compatibility with the pretreatment (prewashing + Ca2+ flocculation) and to ultimately identify the optimal cell... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell lysis; DNA extraction method; DNA purity; Terminal restriction fragment length polymorphism; Fungal community. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400817 |
