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A procedure to evaluate the efficiency of utilization of dietary amino acid for poultry Animal Sciences
Silva, Edney Pereira da; Universidade Estadual Paulista “Júlio de Mesquita Filho”; Sakomura, Nilva Kazue; Universidade Estadual Paulista “Júlio de Mesquita Filho”; Dorigam, Juliano Cesar de Paula; Universidade Estadual Paulista “Júlio de Mesquita Filho”; Malheiros, Euclides Braga; Universidade Estadual Paulista “Júlio de Mesquita Filho”; Fernandes, Joao Batista Kochenborger; Universidade Estadual Paulista “Júlio de Mesquita Filho”; Araujo, José Anchieta de; Universidade Estadual Paulista “Júlio de Mesquita Filho”.
The aim of this study was to develop a procedure based on Gompertz function to determine the efficiency of utilization of amino acid. The procedure was applied to determine the efficiency of utilization of dietary lysine, methionine+cystine and threonine by growing pullets and based on the efficiencies were estimated the requirements for the growth phase of birds. The Gompertz function was fitted to the data of feed intake, body weight, feather-free body protein weight and feather protein weight of four strains of laying hens in the growth phase. The rates of consumption and daily protein deposition (PD) were calculated. The amino acid deposition was obtained by multiplying the PD by the amino acid concentration in feather protein and feather-free body...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.04.03.01-0 growth curve; Lysine; Methionine+cystine; Protein weight; Pullets; Threonine.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/22013
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Threonine-lysine ratio on the requirements of digestible lysine in diets for broilers Animal Sciences
Ospina-Rojas, Iván Camilo; Universidade Estadual de Maringá; Murakami, Alice Eiko; Universidade Estadual de Maringá; Eying, Cinthia; Universidade Estadual de Maringá; Picoli, Karla Paola; Universidade Estadual de Maringá; Scapinello, Claudio; Universidade Estadual de Maringá; Duarte, Cristiane Regina do Amaral; Universidade Estadual de Maringá.
Two experiments were performed to determine the influence of threonine-lysine (Thr:Lys) ratio on requirements of digestible lysine (Lys) in low crude protein diets for broilers in the growth phase. In the first experiment, a total of 480 Cobb 500 male broilers were distributed in a completely randomized experimental design with four dietary Thr:Lysratio (57.0; 60.5; 64.0 and 67.5%) and with five replicates of 24 birds each. No significant differences were reported in weight gain, feed intake, poultry manure humidity, carcass and meat yields. However, the feed conversion was increased linearly as dietary Thr:Lysratio was increased. In the second experiment, a total of 400 Cobb 500 male broilers were distributed in a completely randomized experimental design...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.04.03.00-1 amino-acid; Lysine; Ideal protein; Threonine.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/22557
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Are high-lysine cereal crops still a challenge? BJMBR
Ferreira,R.R.; Varisi,V.A.; Meinhardt,L.W.; Lea,P.J.; Azevedo,R.A..
The essential amino acids lysine and threonine are synthesized in higher plants via a pathway starting with aspartate that also leads to the formation of methionine and isoleucine. Lysine is one of most limiting amino acids in plants consumed by humans and livestock. Recent genetic, molecular, and biochemical evidence suggests that lysine synthesis and catabolism are regulated by complex mechanisms. Early kinetic studies utilizing mutants and transgenic plants that over-accumulate lysine have indicated that the major step for the regulation of lysine biosynthesis is at the enzyme dihydrodipicolinate synthase. Despite this tight regulation, recent strong evidence indicates that lysine catabolism is also subject to control, particularly in cereal seeds. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lysine; Threonine; Aspartate; Nutritionally improved crops; Methionine; Amino acids.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700002
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Isolation of enzymes involved in threonine biosynthesis from sorghum seeds Braz. J. Plant Physiol.
Ferreira,Renato Rodrigues; Vendemiatti,Ariane; Meinhardt,Lyndel Wayne; Lea,Peter John; Azevedo,Ricardo Antunes.
Cereal seeds are poor in essential amino acids, particularly lysine, tryptophan and threonine. The amino acids lysine and threonine are synthesized in the aspartate pathway. Although most of the enzymes of the aspartate pathway have been isolated and characterized in higher plant species, the metabolism of lysine and threonine is totally unknown in sorghum. We have isolated two enzymes, aspartate kinase (AK) and homoserine dehydrogenase (HSDH) from sorghum. Optimum assay conditions were established for the determination of AK and HSDH activities. The highest level of activity was observed in immature seeds. AK was shown to be inhibited by threonine and lysine indicating the existence of at least two isoenzymes, one sensitive to threonine inhibition and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Aspartate kinase; Homoserine dehydrogenase; Lysine; Threonine.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000200005
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Niveles de lisina y treonina digestible en dietas sorgo-pasta de soya para cerdos en crecimiento. Colegio de Postgraduados
Gutiérrez Hernández, Sandra.
La lisina (lis) y treonina (tre) son el primer y segundo aminoácidos limitantes en las dietas de cerdos para la síntesis de proteína. El objetivo del trabajo fue evaluar el efecto de diferentes niveles de lisina y treonina en dietas sorgo-pasta de soya para cerdos en crecimiento, sobre el comportamiento productivo, características de la canal y la concentración de urea en plasma. En el experimento 1 los tratamientos (T) fueron: T1, 0.83 lis+ 0.52 tre; T2, 0.83 lis+ 0.62 tre; T3, 0.93 lis+ 0.52 tre; T4, 0.93 lis+ 0.62 tre). Se utilizaron 36 cerdos (19 hembras y 17 machos castrados) híbridos (Landrace×Yorkshire×Pietrain) con peso promedio inicial de 27,65 ± 5,65 kg durante cuatro semanas, distribuidos en un diseño completamente al azar con arreglo factorial...
Palavras-chave: Lisina; Treonina; Comportamiento productivo; Características de la canal; Lysine; Threonine; Productive performance; Carcass characteristics; Ganadería.
Ano: 2014 URL: http://hdl.handle.net/10521/2288
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A moderate threonine deficiency affects gene expression profile, paracellular permeability and glucose absorption capacity in the ileum of piglets ArchiMer
Hamard, Alice; Mazurais, David; Boudry, Gaelle; Le Huerou-luron, Isabelle; Seve, Bernard; Le Floc'H, Nathalie.
High dietary threonine extraction by the digestive tract suggests that threonine contributes to maintain gut physiology. In the present study, we evaluated the impact of a low (6.5 g of threonine/kg diet; LT group) or a control well-balanced threonine diet (9.3 g of threonine/kg diet; C group) given to piglets for 2 weeks on ileal permeability and Na+-dependant glucose absorption capacity in Ussing chambers. The paracellular permeability was significantly increased in the ileum of LT compared to C piglets (P=.017). The Na+-dependent glucose absorption capacity showed a nonsignificant increase in the LT piglets. In addition, we analysed ileal gene expression profiles in the LT and C groups using porcine multitissue cDNA microarrays. Compared to the C...
Tipo: Text Palavras-chave: Threonine; Small intestine; Paracellular permeability; Gene expression.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00014/12531/9425.pdf
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Layer Performance Recovery After Feeding Amino Acid-deficient Diets Rev. Bras. Ciênc. Avic.
Faria,DE; Harms,RH; Russell,GB.
Three hundred and twenty Hy-Line W36<FONT FACE=Symbol>Ò</FONT> commercial laying hens, 39 weeks of age, were used to determine the sequence and the length of time needed for hens to recover performance characteristics after an eight-week period under graded levels of threonine deficiency. Eight experimental diets with Thr levels ranging from 0.35 to 0.53% were randomly fed with eight replicates of five hens each. After the previous experiment, the hens were fed a control diet (0.53% Thr) for a four-week period. Feed consumption (FC), energy intake (EI), egg production (EP), egg weight (EW), egg mass (EM), and body weight (BW) were evaluated. All performance characteristics were impaired on Thr deficient diets. The recovery sequence order was FC...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acid deficiency; Egg production; Laying hens; Threonine.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2002000200007
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The Benefit of L-Threonine Supplementation on Growth Performance, Carcass Characteristics, Intestinal Morphology and Litter Quality of Broilers. Rev. Bras. Ciênc. Avic.
Al-Sagan,AA; Khalil,S; Smith,MP.
ABSTRACT This study was carried out to investigate the effect of dietary L-Threonine supplementation on broiler performance, carcass characteristics, duodenal histo-morphology and litter analysis. It was hypothesised that the overall broiler performance would be improved in threonine adequate diets with reduced environmental impact when using L-threonine supplementation. A total of 144-sexed Ross 308 broiler chicks were randomly allocated into 3 dietary treatments. Each treatment was replicated 6 times with 8 birds per replicate (4 male and 4 female). Treatment group 1 served as threonine deficient group, fed with a diet with all limiting amino acids met except threonine as no synthetic form was supplied. Treatment group 2 served as threonine adequate high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crude protein; Threonine; Broilers; Carcass characteristics; Litter quality; Intestinal morphology.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400753
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Distribution of soluble amino acids in maize endosperm mutants Scientia Agricola
Toro,Alejandro Alberto; Medici,Leonardo Oliveira; Sodek,Ladaslav; Lea,Peter John; Azevedo,Ricardo Antunes.
For human nutrition the main source of vegetable proteins are cereal and legume seeds. The content of total soluble amino acids in mature endosperm of wild-type, opaque and floury maize (Zea mays L.) mutants were determined by HPLC. The total absolute concentration of soluble amino acids among the mutants varied depending on the mutant. The o11 and o13 mutants exhibited the highest average content, whereas o10, fl3 and fl1 exhibited the lowest average content. In general, the mutants exhibited similar concentrations of total soluble amino acids when compared to the wild-type lines, with the clear exception of mutants o11 and fl1, with the o11 mutant exhibiting a higher concentration of total soluble amino acids when compared to its wild-type counterpart...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Lysine; Threonine; Methionine.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000100014
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Determination of aspartate kinase activity in maize tissues Scientia Agricola
Ferreira,Renato Rodrigues; Vendemiatti,Ariane; Gratão,Priscila Lupino; Lea,Peter John; Azevedo,Ricardo Antunes.
Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Aspartate pathway; Maize; Lysine; Threonine; Methionine.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200015
Registros recuperados: 10
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