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| Melani,Rafael D.; Nogueira,Fabio C. S.; Domont,Gilberto B.. |
| Abstract The protein composition of animal venoms is usually determined by peptide-centric proteomics approaches (bottom-up proteomics). However, this technique cannot, in most cases, distinguish among toxin proteoforms, herein called toxiforms, because of the protein inference problem. Top-down proteomics (TDP) analyzes intact proteins without digestion and provides high quality data to identify and characterize toxiforms. Denaturing top-down proteomics is the most disseminated subarea of TDP, which performs qualitative and quantitative analyzes of proteoforms up to ~30 kDa in high-throughput and automated fashion. On the other hand, native top-down proteomics provides access to information on large proteins (> 50 kDA) and protein interactions... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Venomics; Toxiforms; Top-down proteomics; Denaturing top-down proteomics; Native top-down proteomics. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100209 |
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| Calvete,Juan J.; Petras,Daniel; Calderón-Celis,Francisco; Lomonte,Bruno; Encinar,Jorge Ruiz; Sanz-Medel,Alfredo. |
| Abstract In this paper we discuss recent significant developments in the field of venom research, specifically the emergence of top-down proteomic applications that allow achieving compositional resolution at the level of the protein species present in the venom, and the absolute quantification of the venom proteins (the term “protein species” is used here to refer to all the different molecular forms in which a protein can be found. Please consult the special issue of Jornal of Proteomics “Towards deciphering proteomes via the proteoform, protein speciation, moonlighting and protein code concepts” published in 2016, vol. 134, pages 1-202). Challenges remain to be solved in order to achieve a compact and automated platform with which to routinely carry out... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venomics; Top-down proteomics; Top-down venomics; Protein species-resolved venomics; Absolute quantification; Inductively coupled plasma mass spectrometry. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100205 |
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