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| Ma,Min Min; Jiang,Dan; Li,Shuang Ya; Wu,Yuan; Meng,Ju; Huang,Jiang Yao. |
| ABSTRACT Adulterant herbal materials are threats to import and export trade and consumer safety. In this study, we established a simple and rapid examination system for the detection of Phellodendron chinense Schneid. Two detection methods, real-time fluorescence quantitative PCR (real-time PCR) and loop-mediated isothermal amplification (LAMP), were developed for traditional Chinese medicine detection, and their specificity and sensitivity were compared. The DNA of P. chinense was extracted and its special periods amplified with designed primers. Real-time PCR and LAMP experiments were conducted to test the specificity of primers in contrast to other similar species. The template concentration was diluted from 101 ng/µL to 10-5 ng/µL in order to contrast... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Traditional Chinese medicine; Real-time PCR; Phellodendron chinense Schneid; LAMP; Specificity; Sensitivity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100314 |
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| Zhao,Hui; Li,Yao; Xu,QiuXia; Peng,Fu; Zhao,JinShuang; Webb,R. Clinton; Peng,Cheng; Yu,ChengHao. |
| Uterine leiomyomas (ULs) are benign monoclonal tumors that arise from the underlying myometrial tissue in the uterus. Effective therapies are still lacking because of poor understanding of the pathophysiology and epidemiology. Hence, it is urgent to establish efficient animal models to screen novel anti-UL therapies. In this study, for the first time, traditional Chinese medicine and Western medicine were combined to establish an animal model of ULs in rats. In order to evaluate the function and value of the novel model, it was compared with other models. The long-term and short-term rat models for ULs were established using progesterone and diethylstilbestrol. Rats in Qi stagnation and blood stasis group were injected with epinephrine hydrochloride and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Uterine leiomyomas; Animal model; Traditional Chinese medicine. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000900603 |
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| Cai,Xin-jun; Wang,Zeng; Xu,Ying-ying; Ye,Lihong; Cao,Jia-wei; Ni,Jian-jun; Wu,Ting-ting. |
| The Ruanjian Sanjie Decoction (RSD) is a traditional Chinese medicine (TCM) formulation consisting of Spica Prunellae, Pseudobulbus Cremastrae Seu Pleiones, Concha Ostreae and Semen Coicis, and widely used as an adjuvant in anti-cancer therapy. The aim of this study was to determine the effects of RSD on the extracellular matrix (ECM) of tumors, and on the efficacy of anti-cancer nano-formulations in a tumor-bearing mouse model. The mice were treated with triptolide encapsulated in PEG-modified liposomes (TP-PEG-LPs), either alone or in combination with RSD. The combination treatment significantly retarded tumor growth relative to the untreated controls, indicating the potent adjuvant effect of RSD in targeted anti-cancer therapy. In addition, RSD also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Traditional Chinese medicine; Ruanjian Sanjie Decoction; Tumor matrix; Anti-tumor efficacy; Nano-formulations. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100605 |
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| Feng,Tu; Liu,Shuang; He,Xing-jin. |
| Traditionally, the authentication of the traditional Chinese medicines (TCM), Angelica sinensis, is based on slightly different morphological characters and complex compounds. Usually, those methods are simultaneously affected by several factors, leading to subtle and ambiguous results. In this study, the internal transcribed spacer (ITS) regions of A. sinensis and seven other Angelica species used as adulterants were sequenced. A pair of specific primers was designed from the polymorphic ITS regions to distinguish A. sinensis from the adulterants and regional substitutes. These ITS-derived primers amplified approximately 520 bp specific fragments from the adulterants, whereas no products was amplified with the DNA of A. sinensis. We tested eight... |
| Tipo: Journal article |
Palavras-chave: Angelica sinensis; Internal transcribed spacer; Molecular authentication; Specific primers; Traditional Chinese medicine. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100009 |
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| Song,Y; Zhu,J; Wang,T; Zhang,C; Yang,F; Guo,X; Liu,P; Cao,H; Hu,G. |
| ABSTRACT This study has the objective of investigating the effects of traditional Chinese medicine prescriptions (TCM) on serum lipid, abdominal and hepatic fat percentage, cholesterol content in eggs, and mRNA expression of genes apoA I and apoB100. One hundred and thirty five healthy (300-day-old) layers were randomly assigned to three treatments. The hens in control group were fed with the basal diet. The hens in the experimental groups (TCM 1 and TCM 2) were fed with the basal diet supplemented with 1% TCM 1 and 1% TCM 2 respectively over a period of 60 days. Laying performance and the serum parameters relevant to fat metabolism were measured. The results showed that no significant differences were found in average daily feed intake and egg weight... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laying hens; Cholesterol; Traditional Chinese medicine; Lipid metabolism; Apolipoprotein.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000100153 |
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