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Fonseca,María Isabel; Tejerina,Marcos Raúl; Sawostjanik-Afanasiuk,Silvana Soledad; Giorgio,Ernesto Martin; Barchuk,Mónica Lucrecia; Zapata,Pedro Darío; Villalba,Laura Lidia. |
Abstract Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts of laccase and MnP; when Cu2+ was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: White rot fungi; Trametes; Laccase; Manganese peroxidase; Cu2+ response. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200287 |
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