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	Provedor de dados BJMBR (1) Autor Margis,R. (1) Vidal,M.S. (1) Palavra-chave Andean potato mottle virus (1) Coat protein (1) Transactivation (1) Virus assembly (1) Yeast two-hybrid system (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2002 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última The 42-kDa coat protein of Andean potato mottle virus acts as a transcriptional activator in yeast BJMBR Vidal,M.S.; Margis,R.. Interactions of viral proteins play an important role in the virus life cycle, especially in capsid assembly. Andean potato mottle comovirus (APMoV) is a plant RNA virus with a virion formed by two coat proteins (CP42 and CP22). Both APMoV coat protein open reading frames were cloned into pGBT9 and pGAD10, two-hybrid system vectors. HF7c yeast cells transformed with the p9CP42 construct grew on yeast dropout selection media lacking tryptophan and histidine. Clones also exhibited ß-galactosidase activity in both qualitative and quantitative assays. These results suggest that CP42 protein contains an amino acid motif able to activate transcription of His3 and lacZ reporter genes in Saccharomyces cerevisiae. Several deletions of the CP42 gene were cloned into... Tipo: Info:eu-repo/semantics/article Palavras-chave: Andean potato mottle virus; Virus assembly; Coat protein; Transactivation; Yeast two-hybrid system. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400001 Registros recuperados: 1 Primeira ... 1 ... Última

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