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| Registros recuperados: 43 | |
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| Vogel,R.F.; Pavlovic,M.; Hörmann,S.; Ehrmann,M.A.. |
| Lactobacillus sanfranciscensis is a Gram-positive lactic acid bacterium used in food biotechnology. It is necessary to investigate many aspects of a model organism to elucidate mechanisms of stress response, to facilitate preparation, application and performance in food fermentation, to understand mechanisms of inactivation, and to identify novel tools for high pressure biotechnology. To investigate the mechanisms of the complex bacterial response to high pressure we have analyzed changes in the proteome and transcriptome by 2-D electrophoresis, and by microarrays and real time PCR, respectively. More than 16 proteins were found to be differentially expressed upon high pressure stress and were compared to those sensitive to other stresses. Except for one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: High pressure; Lactobacillus sanfranciscensis; Proteome; Transcriptome; Stress response. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800013 |
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| Hindmarch,C.C.T.; Franses,P.; Goodwin,B.; Murphy,D.. |
| The supraoptic nucleus (SON) is part of the central osmotic circuitry that synthesises the hormone vasopressin (Avp) and transports it to terminals in the posterior lobe of the pituitary. Following osmotic stress such as dehydration, this tissue undergoes morphological, electrical and transcriptional changes to facilitate the appropriate regulation and release of Avp into the circulation where it conserves water at the level of the kidney. Here, the organisation of the whole transcriptome following dehydration is modelled to fit Zipf's law, a natural power law that holds true for all natural languages, that states if the frequency of word usage is plotted against its rank, then the log linear regression of this is -1. We have applied this model to our... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transcriptome; Supraoptic nucleus; Dehydration; Zipf; Microarray; Vasopressin. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201000 |
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| Vieira,Luiz Gonzaga Esteves; Andrade,Alan Carvalho; Colombo,Carlos Augusto; Moraes,Ana Heloneida de Araújo; Metha,Ângela; Oliveira,Angélica Carvalho de; Labate,Carlos Alberto; Marino,Celso Luis; Monteiro-Vitorello,Claúdia de Barros; Monte,Damares de Castro; Giglioti,Éder; Kimura,Edna Teruko; Romano,Eduardo; Kuramae,Eiko Eurya; Lemos,Eliana Gertrudes Macedo; Almeida,Elionor Rita Pereira de; Jorge,Érika C.; Albuquerque,Érika V. S.; Silva,Felipe Rodrigues da; Vinecky,Felipe; Sawazaki,Haiko Enok; Dorry,Hamza Fahmi A.; Carrer,Helaine; Abreu,Ilka Nacif; Batista,João A. N.; Teixeira,João Batista; Kitajima,João Paulo; Xavier,Karem Guimarães; Lima,Liziane Maria de; Camargo,Luis Eduardo Aranha de; Pereira,Luiz Filipe Protasio; Coutinho,Luiz Lehmann; Lemos,Manoel Victor Franco; Romano,Marcelo Ribeiro; Machado,Marcos Antonio; Costa,Marcos Mota do Carmo; Sá,Maria Fátima Grossi de; Goldman,Maria Helena S.; Ferro,Maria Inês T.; Tinoco,Maria Laine Penha; Oliveira,Mariana C.; Van Sluys,Marie-Anne; Shimizu,Milton Massao; Maluf,Mirian Perez; Eira,Mirian Therezinha Souza da; Guerreiro Filho,Oliveiro; Arruda,Paulo; Mazzafera,Paulo; Mariani,Pilar Drummond Sampaio Correa; Oliveira,Regina L.B.C. de; Harakava,Ricardo; Balbao,Silvia Filippi; Tsai,Siu Mui; Mauro,Sonia Marli Zingaretti di; Santos,Suzana Neiva; Siqueira,Walter José; Costa,Gustavo Gilson Lacerda; Formighieri,Eduardo Fernandes; Carazzolle,Marcelo Falsarella; Pereira,Gonçalo Amarante Guimarães. |
| Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to the Rubiaceae family which includes other important plants. The genus contains about 100 species but commercial production is based only on two species, Coffea arabica and Coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. The Brazilian Coffee Genome Project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. We have single-pass sequenced a total of 214,964 randomly picked clones from 37 cDNA libraries of C. arabica, C. canephora and C. racemosa,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea; CDNA; EST; Transcriptome. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100008 |
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| He,Ruishi; Zhu,Xinxin; Li,Qiaoyun; Jiang,Yumei; Yu,Dongyan; Sun,Yulong; Liang,Xiaolong; Ni,Yongjing; Niu,Jishan. |
| ABSTRACT: Wheat (Triticum aestivum L.) stem development significantly affects grain yield. The dwarf plants (D) of wheat mutant dms was less than 30cm. Here, we were to explore the molecular basis for the restrained stem development of the dwarf plants. The results were reached by compare the young spikes and stems transcriptomes of the tall (T) and D plants of mutant dms. We identified 663 genes highly expressed in stem tips. We identified 997 differentially expressed genes (DEGs) in stem tips between T and D, 403 DEGs were significantly related with stem development. Most biological processes in stem tips on dwarf plants were significantly suppressed, such as phytohormone signaling etc. The sequencing analysis results were confirmed by quantitatively... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Wheat (Triticum aestivum L.); Dms mutant; Transcriptome; Stem development.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200401 |
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| Xiaojun,Fan; Chun,Yang; Jianhong,Liu; Chang,Zhang; Yao,Li. |
| Abstract The Asian gypsy moth (Lymantria dispar) is a serious pest of forest and shade trees in many Asian and some European countries. However, there have been few studies of L. dispar genetic information and comprehensive genetic analyses of this species are needed in order to understand its genetic and metabolic sensitivities, such as the molting mechanism during larval development. In this study, high-throughput sequencing technology was used to sequence the transcriptome of the Asian subspecies of the gyspy moth, after which a comprehensive analysis of chitin metabolism was undertaken. We generated 37,750,380 high-quality reads and assembled them into contigs. A total of 37,098 unigenes were identified, of which 15,901 were annotated in the NCBI... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Asian gypsy moth; Chitin metabolism; Transcriptome; Unigenes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100160 |
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| Amaral,Alexandre Morais do; Saito,Daniel; Formighieri,Eduardo Fernandes; Rabello,Edenilson; Souza,Adriane N. de; Silva-Stenico,Maria Estela; Tsai,Siu Mui. |
| Pleiotropic drug resistance (PDR) proteins, a subfamily of the ATP-binding cassette (ABC) transporters, have been recently shown to play a role in plant defense against biotic and abiotic stresses. However, nothing is known about their expression in citrus. To investigate the occurrence of PDR homologues in citrus species, we have surveyed EST sequences from different tissues and conditions of the Citrus Expressed Sequence Tags (CitEST) database, through sequence similarity search analyses and inspections for characteristic PDR domains. Multiple sequence alignments, prediction of transmembrane topology and phylogenetic analysis of PDR-like proteins were additionally performed. This study allowed the identification of nine putative proteins showing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ABC transporters; CitEST; Expressed sequence tag; Transcriptome; Stress. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500014 |
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| Fu,Suhong; Lei,Ming; Zhang,Yongqun; Deng,Zhaomin; Shi,Jing; Hao,Doudou. |
| Abstract Dysphania schraderiana is widely distributed in Lhasa (Tibet, China) and used as a traditional medicine. However, the lack of genetic information hinders the understanding of its physiological processes, such as the biosynthesis of secondary metabolites. Herein, we used Illumina Hiseq4000 platform to sequence the transcriptome of flower and leaf tissues from D. schraderiana for the first time. Totally, 40,142 unigenes were assembled from approximately 5.2 million clean reads. All unigenes underwent gene prediction and were subsequently annotated in a NR (NCBI non-redundant protein) database, COG (Clusters of Orthologous Groups of proteins) database, and KEGG (Kyoto Encyclopedia of Genes and Genomes) database. Among the 40,142 unigenes, 2,579 genes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dysphania schraderiana; De novo assembly; Transcriptome; Annotation; Terpenoid biosynthesis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300480 |
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| Rodrigues,Fabiana Aparecida; Marcolino-Gomes,Juliana; Carvalho,Josirlei de Fátima Corrêa; Nascimento,Leandro Costa do; Neumaier,Norman; Farias,José Renato Bouças; Carazzolle,Marcelo Falsarella; Marcelino,Francismar Corrêa; Nepomuceno,Alexandre Lima. |
| Soybean has a wide range of applications in the industry and, due to its crop potential, its improvement is widely desirable. During drought conditions, soybean crops suffer significant losses in productivity. Therefore, understanding the responses of the soybean under this stress is an effective way of targeting crop improvement techniques. In this study, we employed the Suppressive Subtractive Hybridization (SSH) technique to investigate differentially expressed genes under water deficit conditions. Embrapa 48 and BR 16 soybean lines, known as drought-tolerant and -sensitive, respectively, were grown hydroponically and subjected to different short-term periods of stress by withholding the nutrient solution. Using this approach, we have identified genes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; Drought; Transcriptome. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200011 |
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| Sousa,Cristina Soares de; Serrão,José Eduardo; Bonetti,Ana Maria; Amaral,Isabel Marques Rodrigues; Kerr,Warwick Estevam; Maranhão,Andréa Queiroz; Ueira-Vieira,Carlos. |
| The insect fat body is a multifunctional organ analogous to the vertebrate liver. The fat body is involved in the metabolism of juvenile hormone, regulation of environmental stress, production of immunity regulator-like proteins in cells and protein storage. However, very little is known about the molecular mechanisms involved in fat body physiology in stingless bees. In this study, we analyzed the transcriptome of the fat body from the stingless bee Melipona scutellaris. In silico analysis of a set of cDNA library sequences yielded 1728 expressed sequence tags (ESTs) and 997 high-quality sequences that were assembled into 29 contigs and 117 singlets. The BLAST X tool showed that 86% of the ESTs shared similarity with Apis mellifera (honeybee) genes. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fat body; Gene expression; Melipona; Stingless bees; Transcriptome. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200022 |
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| Barbosa,Aulus Estevão; Marinho,Paulo. |
| The Eucalyptus Genome Sequencing Project (FORESTs), an initiative from the Brazilian ONSA consortium (Organization for Nucleotide Sequencing and Analysis), has achieved the sequencing of 123.889 EST clones from 18 different cDNA libraries. We have investigated the FORESTs data set to identify EST clusters potentially encoding thioredoxins (TRX). Two types of thioredoxin families described in plants, chloroplastic (TRXm/f/x/y) and cytosolic (TRXh), have been found in the transcriptome. Putative typical TRXs have been identified in fifteen clusters, four m-type, seven h-type, two f-type, one cluster for each x/y-types and one putative homologue of the TDX gene from Arabidopsis thaliana. One cluster presents an atypical active site WCMPS, different from the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eucalyptus; Transcriptome; Thioredoxin. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400008 |
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| Ma,Juan; Gao,Bo; Wang,Rongyan; Li,Xiuhua; Chen,Shulong. |
| Abstract The objective of this study was to identify molecular responses in Ditylenchus destructor to cold and desiccation by means of transcriptomes analyses. A total of 102,517 unigenes were obtained, with an average length of 1,076 bp, in which 58,453 (57%) had a functional annotation. A total of 1154 simple sequence repeats (SSRs) distributed over 1078 unigenes were detected. Gene expression profiles in response to cold and desiccation stress and the expression of specific stress-related genes were compared. Gene ontology analysis and pathway-based analysis were used to further investigate the functions of the differentially expressed genes. The reliability of the sequencing data was verified through quantitative real-time PCR analysis of 19... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Potato rot nematode; Transcriptome; Tolerance; Differentially expressed genes. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100202 |
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| Sá,Maria Eugênia Lisei de; Lopes,Marcus José Conceição; Campos,Magnólia de Araújo; Paiva,Luciano Vilela; Amorim,Regina Maria Santos de; Beneventi,Magda Aparecida; Firmino,Alexandre Augusto Pereira; Sá,Maria Fátima Grossi de. |
| Soybean is an important crop for Brazilian agribusiness. However, many factors can limit its production, especially root-knot nematode infection. Studies on the mechanisms employed by the resistant soybean genotypes to prevent infection by these nematodes are of great interest for breeders. For these reasons, the aim of this work is to characterize the transcriptome of soybean line PI 595099-Meloidogyne javanica interaction through expression analysis. Two cDNA libraries were obtained using a pool of RNA from PI 595099 uninfected and M. javanica (J2) infected roots, collected at 6, 12, 24, 48, 96, 144 and 192 h after inoculation. Around 800 ESTs (Expressed Sequence Tags) were sequenced and clustered into 195 clusters. In silico subtraction analysis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; Root knot nematode; Transcriptome. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200008 |
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| SALGADO, F. F.; GRYNBERG, P.; SOUZA JUNIOR, M. T.. |
| The discovery of noncoding RNAs (ncRNAs) is changed our understanding of genome, transcriptome, and interactions with proteome in a cell. Since the discovery of the first microRNA (miRNA) called lin-4 on Caenorhabditis elegans in 1993, our understanding of gene expression has deepened. Further miRNA discoveries have led to the investigation of new ncRNA families and their function, biogenesis, and interactions with other types of molecules. Recent developments in bioinformatics, public databases, sequencing technologies, and biochemical techniques have increased our understanding of the biogenesis, function, and interactions of ncRNAs. This book provides an overview of current ncRNA research dealing with human diseases and cancer and plant miRNAs. |
| Tipo: Parte de livro |
Palavras-chave: Óleo Vegetal; Oil palm products; Abiotic stress; Salt stress; Transcriptome; Oilseed crops. |
| Ano: 2022 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1148820 |
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| Registros recuperados: 43 | |
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