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| Registros recuperados: 79 | |
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| MENDES, L. W.; RAAIJMAKERS, J. M.; HOLLANDER, M. de; SEPO, E.; EXPÓSITO, R. G.; CHIORATO, A. F.; MENDES, R.; TSAI, S. M.; CARRIÓN, V. J.. |
| Abstract: Background - Plants rely on their root microbiome as the first line of defense against soil-borne fungal pathogens. The abundance and activities of beneficial root microbial taxa at the time prior to and during fungal infection are key to their protective success. If and how invading fungal root pathogens can disrupt microbiome assembly and gene expression is still largely unknown. Here, we investigated the impact of the fungal pathogen Fusarium oxysporum (fox) on the assembly of rhizosphere and endosphere microbiomes of a fox-susceptible and fox-resistant common bean cultivar. -- Results: Integration of 16S-amplicon, shotgun metagenome as well as metatranscriptome sequencing with community ecology analysis showed that fox infections... |
| Tipo: Artigo de periódico |
Palavras-chave: Plant-microbe interaction; Endosphere; Metagenome; Rizosfera; Feijão; Raiz; Bactéria; Controle Biológico; Beans; Rhizosphere bacteria; Transcriptome. |
| Ano: 2023 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156506 |
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| SALGADO, F. F.; GRYNBERG, P.; SOUZA JUNIOR, M. T.. |
| The discovery of noncoding RNAs (ncRNAs) is changed our understanding of genome, transcriptome, and interactions with proteome in a cell. Since the discovery of the first microRNA (miRNA) called lin-4 on Caenorhabditis elegans in 1993, our understanding of gene expression has deepened. Further miRNA discoveries have led to the investigation of new ncRNA families and their function, biogenesis, and interactions with other types of molecules. Recent developments in bioinformatics, public databases, sequencing technologies, and biochemical techniques have increased our understanding of the biogenesis, function, and interactions of ncRNAs. This book provides an overview of current ncRNA research dealing with human diseases and cancer and plant miRNAs. |
| Tipo: Parte de livro |
Palavras-chave: Óleo Vegetal; Oil palm products; Abiotic stress; Salt stress; Transcriptome; Oilseed crops. |
| Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1147999 |
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| BENEVENTI, M. A.; SILVA JUNIOR, O. B. da; SÁ, M. E. L. de; FIRMINO, A. A. P.; AMORIM, R. M. S. de; ALBUQUERQUE, E. V. S.; SILVA, M. C. M. da; SILVA, J. P. da; CAMPOS, M. de A.; LOPES, M. J. C.; TOGAWA, R. C.; PAPPAS JÚNIOR, G. J.; GROSSI DE SÁ, M. F.. |
| Tipo: Separatas |
Palavras-chave: Root-knot nematode; Pyrosequencing; Plant–pathogen interaction; Hormone.; Glycine Max; Transcriptome. |
| Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/979805 |
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| PINHEIRO, T. D. M.; REGO, E. C. S.; ALVES, G. S. C.; FONSECA, F. C. de A.; COTTA, M. G.; ANTONINO, J. D.; GOMES, T. G.; AMORIM, E. P.; FERREIRA, C. F.; COSTA, M. M. do C.; GRYNBERG, P.; TOGAWA, R. C.; MILLER, R. N. G.. |
| Banana (Musa spp.), which is one of the world's most popular and most traded fruits, is highly susceptible to pests and diseases. Pseudocercospora musae, responsible for Sigatoka leaf spot disease, is a principal fungal pathogen of Musa spp., resulting in serious economic damage to cultivars in the Cavendish subgroup. The aim of this study was to characterize genetic components of the early immune response to P. musae in Musa acuminata subsp. burmannicoides, var. Calcutta 4, a resistant wild diploid. Leaf RNA samples were extracted from Calcutta 4 three days after inoculation with fungal conidiospores, with paired-end sequencing conducted in inoculated and non-inoculated controls using lllumina HiSeq 4000 technology. Following mapping to the reference M.... |
| Tipo: Artigo de periódico |
Palavras-chave: Pseudocercospora musae; Sigatoka leaf spot; Musa Acuminata; Biotic stress; Disease resistance; Transcriptome. |
| Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149287 |
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| IVAMOTO, S. T.; DOMINGUES, D. S.; VIEIRA, L. G. E.; PEREIRA, L. F. P.. |
| Cytochrome P450s (P450s) comprise a gene superfamily encoding enzymes that are involved in diverse plant metabolic pathways that produce primary and secondary metabolites such as phenylpropanoids, terpenoids, nitrogen-containing compounds, and plant hormones. They comprise one of the most diverse gene families in plant evolution. Although there are many studies that aim to characterize P450s in plants, there is no report on the characterization of this superfamily in Coffea arabica, where they might be related to plant tolerance to biotic and abiotic stresses, as well as aroma-related compounds. In this study, we report the characterization and annotation of 87 putative P450s from C. arabica obtained from the Brazilian Coffee Genome Project and describe... |
| Tipo: Artigo de periódico |
Palavras-chave: Expressed sequence tags (ESTs); Candidate genes; Coffea arábica; Cytochrome P-450; Transcriptome. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1039979 |
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| CORRÊA, C. L.; MIDORIKAWA, G. E. O.; FERREIRA FILHO, E. X.; NORONHA, E. F.; ALVES, G. S. C.; TOGAWA, R. C.; SILVA JUNIOR, O. B.; COSTA, M. M. do C.; GRYNBERG, P.; MILLER, R. N. G.. |
| Tipo: Artigo de periódico |
Palavras-chave: Lignocellulosic biomass; Biorefinery; Carbohydrate-active enzymes; Aspergillus terreus; Transcriptome. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126336 |
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| STEINDORFF, A. S.; SERRA, L. A.; FORMIGHIERI, E. F.; FARIA, F. P. de; POÇAS-FONSECA, M. J.; ALMEIDA, J. R. M. de. |
| Abstract: Humicola grisea var. thermoidea is a thermophilic ascomycete and important enzyme producer that has an efficient enzymatic system with a broad spectrum of thermostable carbohydrate-active (CAZy) enzymes. These enzymes can be employed in lignocellulose biomass deconstruction and other industrial applications. In this work, the genome of H. grisea var. thermoidea was sequenced. The acquired sequence reads were assembled into a total length of 28.75 Mbp. Genome features correlate with what was expected for thermophilic Sordariomycetes. The transcriptomic data showed that sugar-cane bagasse significantly upregulated genes related to primary metabolism and polysaccharide deconstruction, especially hydrolases, at both pH 5 and pH 8. However, a number... |
| Tipo: Artigo de periódico |
Palavras-chave: Humicola grisea; Genome sequencing; PH regulation; CAZy enzymes; Bagaço; Cana de Açúcar; Variação Genética; Genome; Transcriptome; Sugarcane bagasse; Humicola. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134449 |
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| OLIVEIRA, J. C. de; FORMIGHIERI, E. F.; GARCIA, A. L. B.; MARGARIDO, G. R. A.; CAMPOS, T. de. |
| O uso do amendoim forrageiro em consórcios com gramíneas nas pastagens e como cobertura verde, consorciado com culturas comerciais, tem crescido nos últimos anos. A análise do genoma funcional permite a identificação de genes de interesse agronômico. Assim, o objetivo deste trabalho foi realizar a anotação funcional de genes do transcriptoma de folhas de Arachis pintoi. Dos 98.432 transcritos analisados, 69% apresentaram correspondências com o banco de dados de proteínas do National Center of Biotechnology Information. As classes função molecular (36%) e processo biológico (35,8%) representaram a maioria dos termos de Gene Ontology atribuídos, enquanto o componente celular (28,2%) apresentou menor número. A análise de expressão diferencial identificou... |
| Tipo: Artigo em anais e proceedings |
Palavras-chave: Amendoim forrageiro; Forage peanut; Cacahuetes forrajeros; Anotação funcional; RNA-seq; Transcriptoma; Hojas; Amarillo; Belomonte; Genoma; Folha; Método de Análise; Arachis pintoi; Genome; Transcriptome; Leaves. |
| Ano: 2023 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1157482 |
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| PADILHA, S. F.; IBELLI, A. M. G.; PEIXOTO, J. O.; CANTAO, M. E.; MOREIRA, G. C. M.; FERNANDES, L. T.; TAVERNARI, F. C.; MORES, M. A. Z.; BASTOS, A. P. A.; DIAS, L. T.; TEIXEIRA, R. A.; LEDUR, M. C.. |
| White striping (WS) is a myopathy characterized by the appearance of white stripes parallel to the muscle fibers in the breast of broiler chickens, composed of adipose and connective tissues. This condition causes economic losses and, although common, its etiology remains poorly understood. Hence, the objective was to identify genes and biological mechanisms involved in the early stages of WS using a paternal broiler line that grows slightly slower than commercial ones, at 35 days of age, through the RNA sequencing of the pectoralis major muscle. Thirty genes were differentially expressed between normal and WS-affected chickens, with 23 upregulated and 7 downregulated in the affected broilers. Of these, 14 genes are novel candidates for WS and are... |
| Tipo: Artigo de periódico |
Palavras-chave: Pectoral myopathy; RNA-Seq; Gene expression; Transcriptome. |
| Ano: 2024 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1166611 |
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| GIACHETTO, P. F.; CUNHA, R. C.; NHANI JUNIOR, A.; GARCIA, M. V.; FERRO, J. A.; ANDREOTTI, R.. |
| The success of cattle tick fixation largely depends on the secretion of substances that alter the immune response of the host. The majority of these substances are expressed by the parasite salivary gland and secreted in tick saliva. It is known that hosts can mount immune responses against ticks and bovine European breeds, and bovine industrial crossbreeds are more susceptible to infestations than are Bos indicus cattle. To identify candidates for the development of novel control strategies for the cattle tick Rhipicephalus (Boophilus) microplus, a salivary gland transcriptome analysis of engorged females fed on susceptible or resistant hosts was performed. Using RNA-Seq, transcriptomes were de novo assembled and produced a total of 235,451 contigs with... |
| Tipo: Artigo de periódico |
Palavras-chave: RNA-Seq; Cattle tick; Host-parasite interaction; Sialome; Transcriptome; Cattle. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1119455 |
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| PADILHA, L.; CAIXETA, E. T.; SILVA, F. R. da. |
| New sequencing technologies brought deep probing of transcriptomes (so-called RNAseq) to the reach of individual researches. Analysis of RNAseq sequences, however, depend on the alignment of reads to a reference genome. Some approaches have been proposed to allow de novo assembly of the transcripts, therefore allowing RNAseq to be used on organisms lacking a reference genome. The efficiency of those approaches, however, were tested only with diploid species. Here we propose a methodology to evaluate the results of de novo assembly of allotetraploid Coffea arabica RNAseq reads obtained from libraries of coffee leaves infected by Hemileia vastatrix. Trinity was able to assemble longer transcripts when compared to ABySS, SOAPdenovo and Oases. Moreover, the... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Coffea Arábica; Genoma; Transcriptome. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126751 |
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| ARAKAKI, J. E.; MALAGO JUNIOR, W.; MUDADU, M. de A.; SANTOS, P. M.; FAVERO, A. P.; BORRA, R. C.; VIGNA, B. B. Z.. |
| Drought is one of the abiotic stresses that most affect plant growth and productivity. Grasses of the genus Paspalum are successfully used as turf and forage in Australia, Argentina, Brazil and United States. Paspalum notatum has good forage quality, and P. vaginatum, high tolerance to salinity. In addition, their potential to tolerate drought has been described previously, making them interesting for transcriptome studies under water deficit. The objective of this work was to analyze the gene expression profiles of both species in response to drought. |
| Tipo: Separatas |
Palavras-chave: Expressão gênica; Transcriptoma; Seca; Paspalum Notatum; Gene expression; Drought tolerance; Transcriptome; Paspalum vaginatum. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114149 |
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| ARRAES, F. B. M.; VASQUEZ, D. D. N.; TAHIR, M.; PINHEIRO, D. H.; FAHEEM, M.; FREITAS-ALVES, N. S.; MOREIRA-PINTO, C. E.; MOREIRA, V. J. V.; PAES-DE-MELO, B.; LISEI-DE-SA, M. E.; MORGANTE, C. V.; MOTA, A. P. Z.; LOURENÇO-TESSUTTI, I. T.; TOGAWA, R. C.; GRYNBERG, P.; FRAGOSO, R. da R.; ALMEIDA-ENGLER, J. de; LARSEN, M. R.; GROSSI-DE-SA, M. F.. |
| The root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematodeinfestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean... |
| Tipo: Artigo de periódico |
Palavras-chave: Root-knot nematode; Differential expression; Meloidogyne Incognita; Soja; Glycine Max; Transcriptome; Proteome; Phenylpropanoids. |
| Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148619 |
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| MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L.. |
| Tipo: Separatas |
Palavras-chave: Soja; Resistência à seca; Drought tolerance; Soybeans; Transcriptome. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1079599 |
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| Registros recuperados: 79 | |
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