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	Provedor de dados BJID (1) Autor Bernedo-Navarro,Robert Alvin (1) Bertol,Jéssica Wildgrube (1) Tibo,Luiz Henrique Soares (1) Yano,Tomomasa (1) Palavra-chave Cytotoxin (SPF) (1) Sepsis (1) Transcytosis (1) Transendothelial electrical resistance (1) Tipo do documento Info:eu-repo/semantics/other (1) Ano 2016 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Cytotoxic factor secreted by Escherichia coli associated with sepsis facilitates transcytosis through human umbilical vein endothelial cell monolayers BJID Tibo,Luiz Henrique Soares; Bertol,Jéssica Wildgrube; Bernedo-Navarro,Robert Alvin; Yano,Tomomasa. Abstract Culture supernatant of sepsis-associated Escherichia coli (SEPEC) isolated from patients with sepsis caused loss of intercellular junctions and elongation of human umbilical vein endothelial cells (HUVEC). The cytotoxic factor was purified from culture supernatant of SEPEC 15 (serogroup O153) by liquid chromatography process. PAGE (polyacrylamide gel electrophoresis) showed that the purified SEPEC cytotoxic factor had a molecular mass of ∼150 kDa and consisted of at least two subunits. At the concentration of 1 CD50 (40 μg/mL) did facilitate transcytosis through the HUVEC cells monolayer of SEPEC 15 as much as E. coli K12 within 30 min without affecting cell viability. These results suggest that this cytotoxic factor, named as SPF (SEPEC's... Tipo: Info:eu-repo/semantics/other Palavras-chave: Cytotoxin (SPF); Sepsis; Transcytosis; Transendothelial electrical resistance. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000300014 Registros recuperados: 1 Primeira ... 1 ... Última

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